Physiological and pathological roles of lipogenesis

Foxk1 stimulates adipogenic differentiation via a peroxisome proliferator‐activated receptor gamma 2‐dependent mechanism

“…16,31 PPARγ and SREBP1 mutually regulate each other, thereby facilitating adipocyte differentiation and lipogenesis. 32 Hence, through gain-of-function and loss-of-function analyses, we investigated the specific role of Foxk1 in adipogenesis and lipogenesis and its effect on the expression of associated crucial regulators. Our data showed that enhanced expression of Foxk1 in C3H/10T1/2, ST2 cells and primary BMSCs by transfecting Foxk1 encoding construct or infecting Foxk1 expression lentivirus apparently promoted cell proliferation, adipogenic differentiation, and lipogenesis, as demonstrated by an increased percentage of differentiated adipocytes and upregulated expression of adipogenic differentiation marker genes (C/EBPα, PPARγ, and Fabp4), lipogenic genes (Srebp1c, Acly, Fasn, Scd1, Dgat1, and Dgat2), and the lipid droplet-containing protein Fsp27 (Figures 3, 5, and 6).…”

“…The process of adipogenesis and lipogenesis is tightly regulated by both hormonal and transcriptional cascades, with C/EBP transcription factors (such as C/EBPα) and PPARγ commonly recognized as the main regulatory factors 16,31 . PPARγ and SREBP1 mutually regulate each other, thereby facilitating adipocyte differentiation and lipogenesis 32 . Hence, through gain‐of‐function and loss‐of‐function analyses, we investigated the specific role of Foxk1 in adipogenesis and lipogenesis and its effect on the expression of associated crucial regulators.…”

MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

“…The important role of MSI2 in the regulation of mammary FAs in cows is dependent on changes in FASN. In fatty acid synthesis, FASN can react not only with acetyl-CoA but also with malonyl-CoA to form palmitic acid (16:0) . Palmitate is a key precursor of other long-chain fatty acids, the corresponding enzymes were subsequently prolonged or converted to SFA and MSFA .…”

“…In fatty acid synthesis, FASN can react not only with acetyl-CoA but also with malonyl-CoA to form palmitic acid (16:0). 42 Palmitate is a key precursor of other long-chain fatty acids, the corresponding enzymes were subsequently prolonged or converted to SFA and MSFA. 42 In our results, we found that overexpression and silencing of Msi2 did not echo the changes in some fatty acids, such as C14:0, C17:0, and C17:1, which were stable after overexpression of Msi2, changed significantly after silencing Msi2, and C17:1, which were stable after overexpression of Msi2, changed significantly after silencing Msi2.…”

LOC646762 Is Involved in Adipogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells

“…In addition, a balance is maintained between adipogenic and osteogenic differentiation of mesenchymal bone marrow stem cells (BMSCs) . Adipocyte differentiation is regulated by expression of multiple functional genes, noncoding RNAs, transcription factors, hormones, and signaling pathway molecules and involves a series of biochemical reactions triggered by various hormones and cytokines . Dysregulated adipocyte differentiation is closely related to several diseases such as obesity, diabetes, cardiovascular diseases, and fatty liver; however, its regulatory mechanism remains unclear.…”