MSI2 Modulates Unsaturated Fatty Acid Metabolism by Binding FASN in Bovine Mammary Epithelial Cells

Lyu, Chen-Chen; Meng, Yu; Che, Hao-Yu; Suo, Jin-Long; He, Yun-Tong; Zheng, Yi; Jiang, Hao; Zhang, Jia-Bao; Yuan, Bao

doi:10.1021/acs.jafc.3c07280

Cited by 1 publication

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“…As the main nutrient component of milk, milk fat contains healthy unsaturated fatty acids and fat-soluble micronutrients. , Milk fat is an important source of animal-derived fatty acids and plays an important role in human health and dietary nutrition. , Bovine mammary epithelial cells (BMECs) are the functional units of lactation in the mammary gland of cows, and the milk fat synthesis ability of BMECs directly affects the amount of milk fat in milk. , Therefore, it is of great research value to analyze the mechanism of milk fat synthesis in BMECs and explore how to increase milk fat content in milk.…”

Section: Introductionmentioning

confidence: 99%

ALG5 Regulates STF-62247-Induced Milk Fat Synthesis via the mTOR Signaling Pathway

Meng,

Lyu,

et al. 2024

J. Agric. Food Chem.

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Milk fat content is a critical indicator of milk quality. Exploring the key regulatory genes involved in milk fat synthesis is essential for enhancing milk fat content. STF-62247 (STF), a thiazolamide compound, has the potential to bind with ALG5 and upregulate lipid droplets in fat synthesis. However, the effect of STF on the process of milk fat synthesis and whether it acts through ALG5 remains unknown. In this study, the impact of ALG5 on milk fat synthesis and its underlying mechanism were investigated using bovine mammary epithelial cells (BMECs) and mouse models through real-time PCR, western blotting, Oil Red O staining, and triglyceride analysis. Experimental findings revealed a positive correlation between STF and ALG5 with the ability to synthesize milk fat. Silencing ALG5 led to decreased expression of FASN, SREBP1, and PPARγ in BMECs, as well as reduced phosphorylation levels in the PI3K/AKT/mTOR signaling pathway. Moreover, the phosphorylation levels of the PI3K/AKT/mTOR signaling pathway were restored when ALG5 silencing was followed by the addition of STF. These results suggest that STF regulates fatty acid synthesis in BMECs by affecting the PI3K/AKT/mTOR signaling pathway through ALG5. ALG5 is possibly a new factor in milk fat synthesis.

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