Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants

Hanafy, Rania Samy; Akladious, Samia Ageeb

doi:10.1016/j.jgeb.2018.02.012

Cited by 71 publications

(51 citation statements)

References 30 publications

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“…With refer to irradiation effects, it was appeared that proline content was reduced with increasing either gamma or laser level in both generations. The present findings are similar to those of Hamideldin and Eliwa (2015) and Hanafy and Akladious (2018) used gamma-radiation, also Perveen et al (2011) used laser-ways.…”

Section: Proline Contentsupporting

confidence: 93%

Enhancement of Drought Tolerance in Salvia coccinea Plants by Irradiation with Gamma and Laser Pre-Treatments

El-Sallami

Abdul-Hafeez

Mostafa

et al. 2019

AJAS

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Gamma-and laser-irradiated seeds were grown under drought stress conditions to evaluate the irradiation effects on growth, flowering and some physiological traits of Salvia coccinea plants grown in pots. Pre-sowing gammairradiation at 5, 10, 15, 20 and 25 k.rad or laser irradiation (He-Ne) for 5, 10, 15 and 20 min. exposure time were applied. Soil moisture levels were imposed by irrigating the plants with 100 (control), 80, 60 and 40% of field capacity (F.C.). Results indicated that laser-irradiation was less effective than gamma-irradiation on drought stress. Plants grown under high water stress (40 and 60% F.C.) resulted in significant decreases in all vegetative growth and flowering parameters tested. The decrease in plant growth and flowering quality induced by water deficit was associated with a reduction in leaf relative water content and increases in total phenols, proline and abscisic acid contents in plants. Under water stress, there were significant changes in the activities of the defensive enzymatic system in leaves. The activities of peroxidase and catalase in irradiated plants with gamma or laser were higher at 60% F.C. than in other water stress treatments. In general, it can be concluded that initiated resistance of Salvia coccinea plants under drought stress are enhanced after pre-sowing seed irradiation with gamma at 15-25 k.rad followed by laser for 10 min. exposure time. The most effective combination was gamma-radiation (15-25 k.rad) plus soil moisture content at 80% F.C. This combined treatment protects plants from the deleterious effects of drought. It can tolerate soil water deficit and consume less irrigation water by 30%.

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Section: Proline Contentsupporting

confidence: 93%

Enhancement of Drought Tolerance in Salvia coccinea Plants by Irradiation with Gamma and Laser Pre-Treatments

El-Sallami

Abdul-Hafeez

Mostafa

et al. 2019

AJAS

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“…Low to mediate doses of gamma radiation found to be positively effective on growth parameters and yield of fenugreek crop (Hanafy and Akladious, 2018). Irradiation with low to mediate doses may be profitable for improving physiological and biochemical processes in irradiated plants as recognized earlier (Borzouei et al, 2013).…”

Section: Introductionmentioning

confidence: 81%

“…Concerning the effect of irradiation, Hanafy and Akladious (2018) found that the progressive increase in growth and yield parameters of irradiated fenugreek plants was detected with gamma doses from 25 to 200 Gy, but dose 100 Gy was found to be the most effective one. Also, they recorded growth inhibition of irradiated plants exposed to 400 Gy below that of the un-irradiated control.…”

Section: Growth and Yield Parametersmentioning

confidence: 99%

Rhizobium-Fenugreek Symbiosis Affected by Nitrogen Fertilizer Rates and Seeds Irradiation: A Field Experiment

Soliman

2019

Journal of Soil Sciences and Agricultural Engineering

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Seeds of fenugreek (Trigonella foenum-graecum L.) either irradiated (100 Gy) or non-irradiated were sown under clay soil conditions. Both seeds were inoculated with Rhizobium strains to assess the bacterial-plant symbiosis performance as affected by nitrogen fertilizer rates. Un-inoculated control was also included. 15 N labeled ammonium sulfate enriched with 5% atom excess was applied at 20 and 40 kg N fed-1 , in addition to non-fertilized treatment to trace and quantify portion of N derived from air (Ndfa).Vegetative growth parameters like shoot length, shoot and pod dry weights, 1000-seeds weight, and nodulation criteria (numbers and dry weight) were significantly enhanced by increasing N fertilizer rates, Rhizobium inoculation and irradiation dose. Raising N fertilizer rate had increased the growth attributes as well as nitrogen and phosphorus uptake by pods and shoots. Rhizobium inoculation improved the plant growth and compensated remarkable amount of nitrogen derived from air (Ndfa) to both plant organs. Fenugreek seeds exposed to gamma ray at low dose reflected positive significant response which translated into good growth attributes and nutrients uptake as well as effective Rhizobium-fenugreek symbiosis performance. This combined strategy as low cost effective and safe could be accepted to ensure remarkable fenugreek production with good quality under such given conditions.

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“…Treating plants with highest doses of gamma rays resulted in the highest reduction in content of genomic DNA. Similarly, the appearance or disappearance of bands under gamma irradiation might be considered as molecular markers for radiation processes 34 . It has been exhibited that effects of gamma rays on ISSR fingerprinting might be connected to structural rearrangements in DNA caused by different types of DNA damages 35 .…”

Section: Primer Namementioning

confidence: 99%

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Aly¹,

Eliwa²,

Maraei³

2019

ScienceAsia

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Grains of two bread wheat cultivars (Triticum aestivum), Sids-1 and Sakha-93, were irradiated with gamma rays at dose levels (0.0, 100, 200, and 300 Gy) to study the effects of gamma irradiation on physiological characteristics and genetic variation of wheat. Irradiation dose level of 200 Gy increased chlorophyll a, b, and total chlorophyll contents significantly in both cultivars. Also, proline content increased with increasing irradiation dose level, the highest concentrations were recorded at 300 Gy for Sids-1 and Sakha-93 cultivars as compared to the control. Inter-Simple Sequence Repeat (ISSR) markers have been done to help understanding their genetic differences. Eight ISSR primers (14A, 44B, HB-08, HB-10, HB-11, HB-12, HB-13, and HB-15) exhibited polymorphism with the un-irradiated and irradiated two wheat cultivars. These pimers successfully showed different banding patterns with several amplicons varied from 4 for (14A) to 15 for (HB-10). These 72 amplicons for the two cultivars and an average of 9.0 amplicons with mean of 67.96% polymorphism and resolving power (Rp) of 3.41. It is also cleared that radiation is more effective on Sids-1 cultivar with 55.5% polymorphism than on Sakha-93 cultivar with 51.9% polymorphism. Total amplicons found in Sids-1cultivar were 64, ten of them were unique amplicons (UA): 5 UA(+) and 5 UA(−). Irradiation dose (200 Gy) showed the highest number of UA (3 UA− and 3 UA+) in Sids-1cultivar. While, total amplicons found in Sakha-93 cultivar were 58, eight of them were 5 UA(+) and 3 UA(−). Irradiation dose (300 Gy) showed the highest number of UA as 4 UA+. It could be concluded that gamma irradiation of wheat grains produced an appropriate number of generated variations and that ISSR analysis given a useful molecular marker for the symmetry of the mutants.

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Physiological and molecular studies on the effect of gamma radiation in fenugreek (Trigonella foenum-graecum L.) plants

Cited by 71 publications

References 30 publications

Enhancement of Drought Tolerance in Salvia coccinea Plants by Irradiation with Gamma and Laser Pre-Treatments

Enhancement of Drought Tolerance in Salvia coccinea Plants by Irradiation with Gamma and Laser Pre-Treatments

Rhizobium-Fenugreek Symbiosis Affected by Nitrogen Fertilizer Rates and Seeds Irradiation: A Field Experiment

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

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