Physiological and molecular characterization of atypical lipid-dependent<i>Malassezia</i>yeasts from a dog with skin lesions: adaptation to a new host?

Cafarchia, Claudia; Latrofa, Maria Stefanía; Figueredo, Luciana Aguiar; Machado, Mauro Luís da Silva; Ferreiro, Laerte; Guillot, Jacques; Boekhout, Teun; Otranto, Domenico

doi:10.3109/13693786.2010.531487

Cited by 24 publications

(20 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method is still currently used for differentiation of Malassezia species [1]. Nonetheless, in some cases it may not be accurate enough to achieve a correct identification of atypical strains [7, 9, 23–25]. Therefore, some molecular methods (e.g.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the species Malassezia pachydermatis and re-evaluation of its lipid dependence using a synthetic agar medium

et al. 2017

View full text Add to dashboard Cite

The genus Malassezia includes lipophilic yeasts, which are part of the skin microbiota of various mammals and birds. Unlike the rest of Malassezia species, M. pachydermatis is described as non-lipid-dependent, as it is able to grow on Sabouraud glucose agar (SGA) without lipid supplementation. In this study we have examined the phenotypic variability within M. pachydermatis and confirmed its lipid-dependent nature using a synthetic agar medium. We used a selection of representative non-lipid-dependent strains from different animal species and three atypical lipid-dependent strains of this species, which were not able to grow after multiple passages on SGA. More than 400 lipid-dependent Malassezia isolates from animals were studied in order to detect the three lipid-dependent strains of M. pachydermatis. The identity of the atypical strains was confirmed by DNA sequencing. On the other hand, we have modified the Tween diffusion test, which is widely used in the characterization of these yeasts, by using a synthetic agar-based medium instead of SGA. This modification has proved to be useful for differentiation of M. pachydermatis strains, providing reproducible results and a straightforward interpretation. The finding of these peculiar lipid-dependent strains exemplifies the large variability within the species M. pachydermatis, which involves rare atypical strains with particular growth requirements.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the species Malassezia pachydermatis and re-evaluation of its lipid dependence using a synthetic agar medium

et al. 2017

View full text Add to dashboard Cite

show abstract

“…A similar conclusion was also proposed for M. furfur [58]. Increasing number of Malassezia isolates, which were identified as a lipid-dependent M. pachydermatis or pleomorphic variants of M. furfur, lead to the suggestion about the possibility of genetic material exchange between these two species [19,21].…”

Section: Discussionmentioning

confidence: 66%

Atypical non-lipid-dependent strains of Malassezia furfur

Zinkevičienė¹,

Norkunas

Čitavičius

2012

Open Life Sciences

View full text Add to dashboard Cite

AbstractMembers of the yeast genus Malassezia, including atypical strains, are lipophilic except for Malassezia pachydermatis. New physiological features that characterize atypical Malassezia strains are mainly associated with alteration in Tween assimilation pattern — such isolates still require lipids for growth. We isolated three non-lipid-dependent strains of Malassezia from patients with diagnosed atopic dermatitis (AD). These isolates could not be identified to the species level via their physiological properties. Phylogenetic trees, based on the D1/D2 regions of the 26S rDNA gene sequences and nucleotide sequences of the ITS1-5.8S-ITS2 rRNA region, showed the isolates to belong to Malassezia furfur. Three non-lipid dependent isolates from AD skin were conspecific, and sequences analysis proved them to be M. furfur.

show abstract

“…This was done because phenolic acids cause initial disruption of cell wall to further act at molecular level. Gene sequences procured from GenBank NCBI database were blasted in the blastx programme of NCBI, and amino acid sequences were obtained for the strains (CBS 1878, CBS 7966, CBS 7222, and CBS 7877) used for study [23, 24]. The alignment of the gene sequence (Figure 4) was done by ClustalW analysis, and further phylogeny was constructed (Figure 4) in form of N-J bootstrapped phylogenetic tree [25–27] by MEGA4 software version 4.0 [28].…”

Section: Methodsmentioning

confidence: 99%

Management of Cosmetic Embarrassment Caused byMalasseziaspp. with Fruticose LichenCladiaUsing Phylogenetic Approach

Pandey

Mishra

Tiwari

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

During anti-Malassezia screening of plants by CLSI broth microdilution method, Cladia aggregata (Swartz) Nyl. (family Cladoniaceae), a fruticose lichen from Sikkim (northeast Himalayan region), has been found effective at minimum inhibitory concentrations (mg/mL) of 2.72, 0.63, and 1.28 against yeast-like fungi namely, M. furfur, M. globosa and M. sympodialis, respectively. These test pathogens are responsible for pityriasis versicolor (PV) and seborrheic dermatitis (SD) in humans. We tried to establish the reason for variable MICs against various Malassezia spp. using bioinformatical tools, thereby reducing the cost of the experimentation. This is the first report on anti-Malassezia activity of C. aggregata and thus can serve as a potential source for the development of cosmaceuticals.

show abstract

Physiological and molecular characterization of atypical lipid-dependentMalasseziayeasts from a dog with skin lesions: adaptation to a new host?

Cited by 24 publications

References 34 publications

Characterization of the species Malassezia pachydermatis and re-evaluation of its lipid dependence using a synthetic agar medium

Characterization of the species Malassezia pachydermatis and re-evaluation of its lipid dependence using a synthetic agar medium

Atypical non-lipid-dependent strains of Malassezia furfur

Management of Cosmetic Embarrassment Caused byMalasseziaspp. with Fruticose LichenCladiaUsing Phylogenetic Approach

Contact Info

Product

Resources

About