Physiological and Immunological Effects of Chronic Antigen Exposure in Immunized Guinea Pigs

Andrew, Deborah; Schellenberg, Robert; Hogg, James C.; Hanna, Christopher J.; Paré, Peter D.

doi:10.1159/000233617

Cited by 22 publications

(18 citation statements)

References 5 publications

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“…Deterioration of EAR observed especially in the OA repetitive inhalation group is not likely to be proportional to the serum levels of 71 or IgE in both cases of the present sensitization methods in accordance with another report (23). Andrew et al (23) suggested that the desensitization in EAR is due to the decreased releasability of histamine in low concentrations of challenging antigen.…”

Section: Discussionsupporting

confidence: 88%

“…Andrew et al (23) suggested that the desensitization in EAR is due to the decreased releasability of histamine in low concentrations of challenging antigen. In the present experiments, mepyramine was used for prevention of sudden death, so that the possibility can be excluded.…”

Section: Discussionmentioning

confidence: 99%

“…By repeating sensitization /challenge, no decrease in the level of either r1 or IgE antibody was recognized. (18)(19)(20)(21)(22)(23). If such a model with experimental asthma with chronicity could be made, it would contribute much to asthma research.…”

Section: Change Of Raw Induced By Antigenmentioning

confidence: 99%

“…On the other hand, it is worthwhile to establish a semichronic or chronic experimental model of allergic bronchial asthma for detailed analysis of the disease and the evaluation of the drugs that have been used as a remedy. However, this model has been little studied because repeated antigen challenge causes the pulmonary organ of guinea pigs to become unresponsive (18)(19)(20)(21)(22)(23).…”

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confidence: 99%

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Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

et al. 1997

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ABSTRACT-To develop a model of chronic experimental asthma in guinea pigs, the animal was forced to inhale the mist of a low dose of ovalbumin (OA) adsorbed on fine Al(OH)3 for sensitization once every 4 weeks. The animal was challenged by inhalation with the mist of OA on day 14 after the respective sensitizations. Either the first or the second antigen challenge markedly induced an early asthmatic response (EAR), whereas there was hardly any late asthmatic response (LAR). At the 3rd challenge, LAR also emerged with some severity. These dual responses were consistently observed until the 10th challenge. On the other hand, repeated inhalation/challenge, once every 2 weeks, with OA alone at the same dose tended to lead to the desensitization of the EAR. In addition, LAR was hardly observed throughout the experiments. In both groups, Ti and IgE levels in the serum were elevated by the repetitive antigen inhalations, yet no obvious relationship between these antibody levels and the intensity of either EAR or LAR was recognized. The present results indicate that the asthmatic model with reproducible EAR and LAR developed in this study appears to be very beneficial for the investigation of bronchial asthma and for the assessment of anti-asthma drugs.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Change Of Raw Induced By Antigenmentioning

confidence: 99%

mentioning

confidence: 99%

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Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

et al. 1997

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“…It may be that the amounts of chemical mediators released from the mast cells in response to the 14th antigen challenge are enhanced at the 2nd day but not the 7th day after the 13th antigen challenge. However, when sensitized guinea pigs were chronically exposed to aerosolized antigen, the amount of histamine anaphylactically released from isolated lung fragments was apparently decreased (31). Thus, it is unlikely that potentiation of anaphylactic production and / or release of chemical mediators from mast cells is induced on day 2 but not day 7.…”

Section: Discussionmentioning

confidence: 99%

Acquired Nasal Hyperresponsiveness Aggravates Antigen-Induced Rhinitis in the Guinea Pig

et al. 2003

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Abstract. Whether a state of nasal hyperresponsiveness influences antigen-induced biphasic nasal blockage and sneezing were examined using a guinea pig model of allergic rhinitis. Sensitized animals were challenged with an antigen, Japanese cedar pollen, once every week. Before the 13th challenge, the animals were randomly divided into 2 groups, and then the 13th challenge was performed (Groups A-0 and B-0). The 14th challenge was done on day 2 (Group A-2) and on day 7 (Group B-7) after the 13th challenge, on which nasal hyperresponsiveness was present and absent, respectively. Biphasic nasal blockage and sneezing after the challenge in Group A-2 were more severe than those in Group A-0, while those of Group B-7 were almost the same as those of Group B-0. An anti-histaminic, mepyramine, inhibited sneezing but not the biphasic nasal blockage in Group B-7. A cysteinyl leukotriene (CysLT) antagonist, pranlukast, suppressed the late nasal blockage but not the early blockage and sneezing in Group B-7. In contrast, in Group A-2, mepyramine significantly attenuated not only sneezing but also the early nasal blockage. Pranlukast significantly inhibited both nasal blockage and sneezing in Group A-2. In conclusion, nasal hyperresponsiveness aggravated the antigen-induced nasal responses, to which histamine and CysLTs considerably contributed.

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Comparison of the airway hyperreactivity produced by single and multiple antigen exposures in sensitized guinea pigs

Howell¹,

Sickels²,

Weichman³

1992

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Chronic airway hyperreactivity is a hallmark feature of asthma, but animal models of airway hyperreactivity often utilize a single antigen challenge. Therefore, we compared the airway hyperreactivity produced by single and multiple antigen challenges in ovalbumin-sensitized guinea pigs. Significant (2-fold) leftward shifts in dose-response curves for i.v. methacholine- or LTD4-induced bronchoconstriction in anesthetized and ventilated animals occurred 24 h following a single ovalbumin challenge. This nonspecific airway hyperreactivity was prevented by pretreatment with ketotifen or dexamethasone. However, airway hyperreactivity was no greater 24 h following the last of 3 daily antigen challenges than after 1 challenge and was absent 72 h following one antigen challenge. These results raise concern over the similarity of antigen-induced airway hyperreactivity in guinea pigs to the chronic airway hyperreactivity in asthmatics.

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Physiological and Immunological Effects of Chronic Antigen Exposure in Immunized Guinea Pigs

Cited by 22 publications

References 5 publications

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

Acquired Nasal Hyperresponsiveness Aggravates Antigen-Induced Rhinitis in the Guinea Pig

Comparison of the airway hyperreactivity produced by single and multiple antigen exposures in sensitized guinea pigs

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