Physiological and electrochemical effects of different electron acceptors on bacterial anode respiration in bioelectrochemical systems

Electricity generation in a microbial fuel cell using yogurt wastewater under alkaline conditions

“…The bacterial viability can change from 20% to 95% in the anode biolm in the MFC, depending on the substrate, exoelectrogens, etc. 25,26 Our results indicated that high concentration of organics could inhibit the bacterial activity. The biomass on the anode biolm slightly increased from 1.75 AE 0.14 mg g À1 under the initial COD of 1.0 AE 0.1 g L À1 to 1.91 AE 0.13 and 2.25 AE 0.11 mg g À1 under the COD values of 2.0 AE 0.1 and 5.0 AE 0.5 g L À1 in the yogurt wastewater, respectively.…”

“…Samples in the anode biolms were stained with LIVE/DEAD Baclight staining kit (Molecular Probes, Invitrogen), and visualized using the Confocal laser scanning microscopy (CLSM, LSM 700, Zeiss) by distinguishing bacteria with high (green color) or low (red color) growth activities. 25,26 The biomass measurements on the anode biolm were carried out using Coomassie light blue staining. 22 The composition of bacterial communities in the anode biolms were evaluated aer at least 3 cycles of operation with a stable electricity generation.…”

Electricity Generation by Shewanella decolorationis S12 without Cytochrome c

“…Cyclic voltammetry (CV) analysis of the MFC anodes was conducted as previously reported (Yang et al, 2014). Electrochemical impedance spectroscopy (EIS) of the MFC anodes was analyzed using an electrochemical workstation (Corrtest, China).…”

Improved performance of microbial fuel cells through addition of trehalose lipids

“…The performance of the MFC significantly depends on the transfer rate from the microbes to the anode. Four mechanisms have been proposed for promoting electron transfer from the exoelectrogen to anode: (1) direct contact with the electrode to convert electrons through cytochrome (Cyt c) and membrane bonding proteins (Yang et al, 2014); (2) electrically conductive flagellin-like nanowires (Lovley et al, 2015) (3) the use of electron shutters, a group of electrochemically active substances (Huang et al, 2016), (4) electro-kinesis, by which electrons are transferred to the electrode surface through a rapid wave of fagellin (Lian et al, 2016). Adding exogenous electron transfer mediators (ETMs) is traditionally used to enhance electron transfer (Szoellosi et al, 2015).…”