Physics of cell adhesion

Bongrand, Pierre; Capo, Christian; Depieds, R

doi:10.1016/0079-6816(82)90007-7

Cited by 84 publications

(45 citation statements)

References 130 publications

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“…As a consequence, a vast literature has arisen over these decades that has been the subject of a number of very good reviews (see, for examples, refs. [2,[4][5][6][7][8][9][10][11][12][13]). This literature attempts to find relationships between material properties (surface chemistry, energy, and morphology) and bioadhesive outcomes by integrating physicochemical and biological approaches to the problem.…”

Section: Introductionmentioning

confidence: 99%

“…In the early years, say 1960's through mid 1980's, there was enthusiasm that cell adhesion could be substantially understood using colloid science, surface chemical, and surface thermodynamic principles (the pioneering 1924 work of Mudd and Mudd [26,27] in bacteria adhesion was possibly the first application of surface thermodynamics to cell adhesion). A number of imaginative physicochemical theories were developed to explain the cell adhesion process [4][5][6][7][8][28][29][30][31][32][33] with the goal of establishing a predictive basis for optimizing biocompatibilityor at least a rational basis for explaining how substratum surface properties so profoundly affect cell-material interactions. Although some of these theories have been useful in separating and weighing the relative importance of various material properties (such as charge, wettability, surface density of cell-binding ligands, etc.…”

Section: Introductionmentioning

confidence: 99%

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Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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“…This is obviously dependent on the conditions of cell-cell encounter: (i) In the simplest experimental setup, contact is obtained by sedimentation. The driving force is thus of the order of a few pN [17,18]. If centrifugation is performed, this may be increased by a factor of 1000.…”

Section: Significance Of Forces At the Cellular Levelmentioning

confidence: 99%

“…When two parallel surfaces approach each other, viscous forces may hamper the thinning of the separating liquid film [17,31,32]. The force Electrostatic repulsion between charged membranes.…”

Section: Hydrodynamic Repulsionmentioning

confidence: 99%

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Steric stabilization and cell adhesion

Solèr

Benoliel

et al. 1996

J Mater Sci: Mater Med

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We present theoretical and experimental arguments supporting the hypothesis that the cell surface glycocalyx may negatively regulate adhesive phenomena. First, it is recalled that a repulsive interaction of several thousands of piconewtons may be generated on a contact area of about 1/100 gm 2 by a combination of electrostatic and entropic forces (steric stabilization). Second, electron microscopical data are reported to provide an estimate of the thickness of the cell coats of murine macrophages and sheep erythrocytes made phagocytable by exposure to glutaraldehyde or specific antibodies. Using conventional carbohydrate staining procedures, it is shown that the total thickness of the electron-dark regions in areas of intercellular contact is lower than the sum of the thicknesses of electron-dark regions on free cell areas. Further, removing negative charges with neuraminidase or neutralizing these charges with polylysine may reduce intermembrane distance in contact areas. Third, it is shown that a decrease of erythrocyte surface charges with neuraminidase increases their adhesion to murine phagocytes under dynamic, not static conditions. It is concluded that a major determinant of steric stabilization is the relative length of adhesion molecules and surface repeller elements, and that repulsion is particularly important under dynamic conditions. Thus, dynamic effects must be included in models of steric stabilization.

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Enantiopure Chiral Poly(glycerol methacrylate) Self‐Assembled Monolayers Knock Down Protein Adsorption and Cell Adhesion

Köwitsch

Zhou

et al. 2013

Adv Healthcare Materials

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Chirality plays a fundamental role not only in biological systems, but also in synthetic materials intended for bio-applications. Self-assembled monolayers (SAMs) are prepared on gold surfaces through a "grafting to" method from racemic or enantiopure chiral poly(glycerol methacrylate)s (PGMA(rac), PGMA(R), and PGMA(S)), having a thiol endgroup. Such SAMs constitute a chemically and structurally well-defined model substrate for studying protein adsorption and cell adhesion as a function of the polymer chirality. Surface plasmon resonance measurements reveal that PGMA SAMs greatly reduce the adsorption of bovine serum albumin (BSA) compared to bare gold surfaces. Interestingly, enantiopure SAMs based on PGMA(R) or PGMA(S) show a significantly larger reduction in BSA adsorption than PGMA(rac)-covered surfaces. Studies with the monocytic cell line THP-1 show a similar relationship between enantiopurity of PGMA SAMs and the extent of cell adhesion. Ellipsometry and Raman spectroscopy measurements indicate that SAMs formed by PGMA(rac) have a higher grafting density compared to SAMs of PGMA(R) and PGMA(S). This seems to be due to the ability of PGMA(rac) to form more intermolecular hydrogen bonds among polymer chains compared to the enantiopure PGMAs. Circular dichroism spectroscopy provide evidence that enantiopure polymers adopt a chiral ordered conformation, most likely helical, in aqueous solutions. It is concluded that a higher water content of SAMs formed by enantiopure PGMA(S) and PGMA(R) SAMs arises from the macromolecular chiral conformation adopted by their enantiopure PGMA chains, and it is the decisive reason for the reduced BSA adsorption and cell adhesion as compared to PGMA(rac) SAMs.

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Physics of cell adhesion

Cited by 84 publications

References 130 publications

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Steric stabilization and cell adhesion

Enantiopure Chiral Poly(glycerol methacrylate) Self‐Assembled Monolayers Knock Down Protein Adsorption and Cell Adhesion

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