Physicochemical, techno-functional, and antioxidant properties of a novel bacterial exopolysaccharide in cooked beef sausage

Trabelsi, Imen; Ktari, Naourez; Triki, Mehdi; Bkhairia, Intidhar; Slima, Sirine Ben; Aydi, Sameh Sassi; Aydi, Samir; Asehraou, Abdeslam; Salah, Riadh Ben

doi:10.1016/j.ijbiomac.2017.12.127

Cited by 37 publications

(19 citation statements)

References 37 publications

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“…The UV‐visible absorbance variations revealed a maximum value which confirmed the polysaccharide identification. This corroborates with previous results (Ben Slima et al., 2018; Hamzaoui et al., 2020; Trabelsi et al., 2018). LmPS broadly exhibited hydroxyl chemical groups (Wu, 2009).…”

Section: Discussionsupporting

confidence: 94%

Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4‐induced liver injury in rats

Hsouna

Hfaiedh

Slima

et al. 2022

Food Science & Nutrition

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The aim of the present study was to investigate the extraction and the characterization of a novel heteropolysaccharide from Tunisian halophyte Lobularia maritima (LmPS). We were also interested in its antioxidant, anti‐inflammatory, and hepatoprotective effects on carbon tetrachloride (CCl4)‐induced liver injury in rats. LmPS physicochemical properties were evaluated by thin‐layer chromatography (TLC), high‐performance liquid chromatography (HPLC), thermogravimetric analysis (TGA), and UV absorption. According to TLC and HPLC results, LmPS was a heteropolysaccharide composed of glucose, galactose, and xylose. Its molecular weight was 130.62 kDa. This heteropolysaccharide was characterized by a significant antioxidant potential and was efficient against oxidative stress and CCL4‐induced hepatotoxicity in rat Wistar models (n = 8) treated with a single dose of LmPS 250 mg/kg of body weight. This was evidenced by a significant increase in serum marker enzymes specially aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH). The cytokines released after stimulation of rats with LmPS showed high anti‐inflammatory profiles with an increased rate of interleukine‐10 (IL‐10) with 0.03 pg/mL compared to animals treated only with CCl4. On the contrary, we noticed a decrease of the other cytokines (tumor necrosis factor α: TNF‐α, interleukine‐6: IL‐6, transforming growth factor beta 1: TGF‐β1) with average concentration values of <0.2, 0.1, and 0.04 pg/mL, respectively. Besides, histopathological examinations revealed that CCl4 causes acute liver damage, characterized by extensive hepatocellular necrosis, vacuolization, and inflammatory cell infiltration, as well as DNA fragmentation. LmPS administration at a dose of 250 mg/kg resulted in a significant hepatoprotection, evidenced by a reduction of CCl4‐induced oxidative damage for all tested markers. These findings eagerly confirmed that LmPS was effective in the protection against CCl4‐induced hepatotoxicity and genotoxicity. It, therefore, suggested a potential therapeutic use of this polysaccharide as an alternative medicine for patients with acute liver diseases.

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Section: Discussionsupporting

confidence: 94%

Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4‐induced liver injury in rats

Hsouna

Hfaiedh

Slima

et al. 2022

Food Science & Nutrition

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“…This indicates that our study EPS might have a future application as an additive or texture enhancer for low-moisture baked foods. The OHC in our study is much higher than the reported OHC for EPS produced by Weissella confusa with 5.1% [ 94 ] and Lactobacillus EPS with 15.9% [ 92 ]. However, it is similarly lower than chitosan with 635% [ 95 ].…”

Section: Discussioncontrasting

confidence: 74%

“…Previously, B . licheniformis EPS showed 98.8% WHC [ 91 ], which is more compared with another earlier report on Lactobacillus EPS which only showed 8.95% WHC [ 92 ]. Our study EPS produced by hot-spring-origin B .…”

Section: Discussionmentioning

confidence: 71%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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Extremophilic microorganisms often produce novel bioactive compounds to survive under harsh environmental conditions. Exopolysaccharides (EPSs), a constitutive part of bacterial biofilm, are functional biopolymers that act as a protecting sheath to the extremophilic bacteria and are of high industrial value. In this study, we elucidate a new EPS produced by thermophilic Bacillus haynesii CamB6 from a slightly acidic (pH 5.82) Campanario hot spring (56.4 °C) located in the Central Andean Mountains of Chile. Physicochemical properties of the EPS were characterized by different techniques: Scanning electron microscopy- energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D and 2D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). The EPS demonstrated amorphous surface roughness composed of evenly distributed macromolecular lumps. GPC and HPLC analysis showed that the EPS is a low molecular weight heteropolymer composed of mannose (66%), glucose (20%), and galactose (14%). FTIR analysis demonstrated the polysaccharide nature (–OH groups, Acetyl groups, and pyranosic ring structure) and the presence of different glycosidic linkages among sugar residues, which was further confirmed by NMR spectroscopic analyses. Moreover, D-mannose α-(1→2) and α-(1→4) linkages prevail in the CamB6 EPS structure. TGA revealed the high thermal stability (240 °C) of the polysaccharide. The functional properties of the EPS were evaluated for food industry applications, specifically as an antioxidant and for its emulsification, water-holding (WHC), oil-holding (OHC), and flocculation capacities. The results suggest that the study EPS can be a useful additive for the food-processing industry.

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“…The WHC of Leptolyngbya EPSs is in the range of those reported for bacterial ones; 117 up to 134% (Yang et al 2019) while the OHC is much higher than that reported for EPS produced by bacteria (Devi et al 2016;Trabelsi et al 2018). The EPSs ability to hold and entrap water and oil molecules were related to the high molecular weight porous structure of polymer chains (Insulkar et al 2018;Trabelsi et al 2018;Gan et al 2020). The peptidic moieties and deoxysugar rhamnose contribute hydrophobic behavior to otherwise hydrophilic macromolecules (Khattar et al 2010).…”

Section: Discussionmentioning

confidence: 88%

“…These properties are highly considered in the food industry particularly as fat adsorber and flavor retention (Insulkar et al 2018). The WHC of Leptolyngbya EPSs is in the range of those reported for bacterial ones; 117 up to 134% (Yang et al 2019) while the OHC is much higher than that reported for EPS produced by bacteria (Devi et al 2016;Trabelsi et al 2018). The EPSs ability to hold and entrap water and oil molecules were related to the high molecular weight porous structure of polymer chains (Insulkar et al 2018;Trabelsi et al 2018;Gan et al 2020).…”

Section: Discussionmentioning

confidence: 95%

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

et al. 2022

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Extracellular polymeric substances (EPSs) produced by the filamentous cyanobacterium identified as Leptolyngbya sp. IkmLPT16 were isolated and characterized chemically, and their antioxidant, functional, and rheological properties were studied. The strain produces a significant amount of EPSs (2.15 g L −1 ) conjointly with a biomass production achieved at a maximum of 1.35 g L −1 after nine production days. Chemical analysis of EPSs revealed the presence of mannose (35%), arabinose (24%), glucose (15%), rhamnose (2%), and one uronic acid (8%). Fourier transformed infrared spectrum of EPSs further revealed the presence of νC-N groups indicating the presence of peptide moieties. Elemental analysis of EPSs showed the presence of sulfate groups (S = 0.59%) as inorganic substituents. Functional properties of Leptolyngbya EPSs were determined based on water holding capacity, oil holding capacity, foaming ability, and metal sorption ability. Experimental results showed high water holding capacity (119%), water solubility index (97.43%), and oil holding ability (87.52%), with a strong metal sorption ability and consequent foam stability (22%). The rheological properties of EPSs were comparable with commercial xanthan gum with higher resistance to Temperature. Leptolyngbya sp. EPSs displayed an effective antioxidant activity via directly scavenging free radicals particularly DDPH• (IC 50 = 4 mg. mL −1 against 10 mg. mL −1 for l-ascorbic acid) and •OH (IC 50 = 10 mg. mL −1 against 20 mg. mL −1 for l-ascorbic acid) and as an iron-chelating agent (IC 50 = 40 mg. mL −1 against 60 mg.mL −1 for EDTA). The outcomes of this study demonstrate the potential use of Leptolyngbya sp. EPSs in several food and pharmaceutical applications.

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Physicochemical, techno-functional, and antioxidant properties of a novel bacterial exopolysaccharide in cooked beef sausage

Cited by 37 publications

References 37 publications

Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4‐induced liver injury in rats

Antioxidant and hepatoprotective effects of novel heteropolysaccharide isolated from Lobularia maritima on CCl4‐induced liver injury in rats

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

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