Physicochemical quality evaluation of natural compounds isolated from crude drugs

Abstract

Aconite root has high toxicity caused by diester alkaloids, thus it was necessary to define the limiting value of diester alkaloids used in medicine formulation. To give the quality of ''Processed Aconite Root'' and ''Powdered Processed Aconite Root'' in the Japanese Pharmacopoeia (14th edn, supplement II), we established the official specification and evaluation methods of standard substances. High qualitative grade diester alkaloids, aconitine, hypaconitine, jesaconitine and mesaconitine, which were useful to evaluate the purity of processed aconite root and powdered processed aconite root, were prepared and evaluated for their stability. We studied the physicochemical specification and evaluation methods of these alkaloids. In addition, an ''Aconitum diester alkaloids standard solution for purity'', which was used for the purity test, was prepared, and we also studied its physicochemical specification and evaluation methods. In addition, to evaluate the quality of processed aconite root and powdered processed aconite root, a TLC identification test was established. A monoester alkaloid of benzoylmesaconine hydrochloride was used as the reference standard in the latter test, and we also investigated its physicochemical specification and evaluation methods.

Evaluation of the taste of crude drug and Kampo formula by a taste-sensing system (4): taste of Processed Aconite Root

“…In 1966, however, the monograph was deleted in the revised version of the JP7, probably because of this crude drug's ambiguous toxicity [10]. Then a variety of studies were performed on component characteristics and quality evaluation of both the raw and processed types of the crude drugs because of the medicinal importance of the tuberous root as a crude drug [2,[11][12][13][14][15][16][17][18][19][20]. Subsequently, in 2004, Processed Aconite Root (PAR) was newly adopted in Supplement II of the JP14, with strict control of the DAs by a purity test [21].…”