Physicochemical Properties of Rhamnolipid Biosurfactant from &amp;lt;i&amp;gt;Pseudomonas aeruginosa&amp;lt;/i&amp;gt; PA1 to Applications in Microemulsions

Mendes, Anderson Nogueira; Filgueiras, Lívia Alves; Pinto, José Carlos; Nele, Márcio

doi:10.4236/jbnb.2015.61007

Cited by 44 publications

(22 citation statements)

References 37 publications

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“…2b). This result is comparable to the recent reports on BS production by P. aeruginosa #112 and PA1 (Gudiña et al 2015;Mendes et al 2015). According to the previous reports, the BSs produced by bacterial strains for instance P. aeruginosa are more effective in decreasing the surface tension of the medium.…”

Section: Production Of Bs and Determination Of Surface Tensionsupporting

confidence: 81%

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Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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Background: Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural characterization of the BS produced by Pseudomonas otitidis P4.Results: An efficient BS producing bacterial strain isolated from the unexplored coal mining site of Chirimiri, India was identified as P. otitidis P4 based on morphological, biochemical and 16S rRNA gene sequence analysis. The surface tension of the culture medium was reduced from 71.18 to 33.4 mN/m. The surface tension and emulsification activity of the BS remained stable over a wide range of temperature, pH and salt concentrations indicating its scope of application in bioremediation, food, cosmetics, and pharmaceutical industries. Structural attributes of BS were determined by biochemical tests, thin layer chromatography (TLC), Fourier transform infrared (FTIR) and nuclear magnetic resonance ( 1 H NMR) spectroscopy analyses, which confirmed the glycolipid nature of BS. Lipid and sugar fractions were the main constituents of the extracted BS. Thermogravimetric (TG) and Differential scanning calorimetry (DSC) analyses showed the thermostable nature of BS. As determined from TGA graph, the degradation temperature of biosurfactant was found to be 280 °C while complete weight loss was observed after 450 °C. Conclusion:The BS isolated from P. otitidis P4 was identified as glycolipid and showed high emulsification activity and stability in a wide range of temperature, pH and salinity which makes it suitable for various industrial and environmental applications.

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Section: Production Of Bs and Determination Of Surface Tensionsupporting

confidence: 81%

“…Carbon and nitrogen sources used in production medium have a great impact on composition as well as on the yield of BSs (Desai and Banat 1997;Perfumo et al 2009;Das et al 2008;Tugrul and Cansunar 2005;Mendes et al 2015).…”

Section: Effect Of Carbon and Nitrogen Sources On Bs Productionmentioning

confidence: 99%

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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“…This improvement is attributed to higher de‐protonation of carboxyl groups of rhamnolipid at elevated pH which cause more interaction between protein and rhamnolipid monomers . In addition, the shape of rhamnolipid monomers would change from large vesicle (r = 60 nm) to small micelle (r = 1.7 nm) by increasing the pH , as presented in Fig. B.…”

Section: Resultsmentioning

confidence: 99%

Rhamnolipid as new bio‐agent for cleaning of ultrafiltration membrane fouled by whey

Aghajani

Rahimpour

Amani

et al. 2018

Engineering in Life Sciences

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In this work, rhamnolipid biosurfactant as an eco‐friendly and biodegradable cleaning agent was produced by Pseudomonas aeruginosa bacteria and was used to evaluate the chemical cleaning efficiency of whey fouled ultrafiltration membranes. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) confirmed the successful synthesis of rhamnolipid. The produced rhamnolipid was compared to chemical cleaners including sodium hydroxide (NaOH), sodium dodecyl sulfate (SDS) and Tween 20. Ultrafiltration membranes used for fouling and cleaning analysis were prepared using phase inversion via immersion precipitation technique. For studying the fouling mechanisms, Hermia's model adapted to cross‐flow was used. From the fouling mechanism experiments, it was found that the complete blocking and cake formation were the dominant fouling mechanisms. The highest values of cleaning efficiency were achieved using rhamnolipid and NaOH as cleaning agents with the flux recovery of 100%, but with considering the low concentration of the rhamnolipid used in the cleaning solution compared to NaOH (0.3 versus 4 g/L for NaOH), its application is preferred.

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“…The PPI-pellet and PPI-dried were dissolved in deionized water at a concentration of 3g.L -1 .A shaker was used and the solution was adjusted to pH 9 using a NaOH solution buffer. After, PPI-pellet and PPI-dried solutions were analyzed by the tensiometer Krüss K100, and the critical micelle concentration (CMC) was calculated [20]. Analyses of interfacial tension were performed using Krüss tensiometer DSA100 at 25°C.…”

Section: Surface and Interfacial Tension Measurementsmentioning

confidence: 99%

Evaluation of surfactant activity and emulsifying of Pea protein isolate (Pisum sativum L.) obtained by the spray dryer

et al. 2018

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This study evaluates the biosurfactant activity and emulsification ability of pea proteins isolated (Pisum sativum L.). The results showed that aqueous solutions containing the pea proteins isolated (PPI) reduce the water surface tension to 47mN/m, approximately. The use of spray dryer does not change the surfactant activity of the PPI because surface tension and CMC will not change. The PPI is thermally stable and can be used in processes that require temperatures between 4°C and 80°C, as industrial processes such as baking, ice cream and other formulation. The protein isolate and pea assessed emulsions with toluene and n-heptane were stable. Emulsions castor and linseed oil were not stable under the conditions reported in this paper. Therefore, despite the pea protein isolate is a surfactant it cannot be used in any industrial process. Both the food industry and in the biotechnology industry should evaluate the conditions for application of protein isolates from pea.

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Physicochemical Properties of Rhamnolipid Biosurfactant from <i>Pseudomonas aeruginosa</i> PA1 to Applications in Microemulsions

Cited by 44 publications

References 37 publications

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Rhamnolipid as new bio‐agent for cleaning of ultrafiltration membrane fouled by whey

Evaluation of surfactant activity and emulsifying of Pea protein isolate (Pisum sativum L.) obtained by the spray dryer

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