Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh, Pallavi; Tiwary, Bhupendra N.

doi:10.1186/s40643-016-0119-3

Cited by 69 publications

(27 citation statements)

References 71 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Fourier transform infrared spectroscopy (FTIR) analysis of the biosurfactant produced from BSP9 clearly reveals the presence of functional groups that typically belong to glycolipids. The spectrum ( Figure 1 ) was consistent with the structure of glycolipids already reported in the literature [ 58 , 59 , 60 ]. The IR spectrum shows absorption bands ranging between 3650–3134 cm −1 , which indicate the presence of free -OH group arising as a result of H bonding of polysaccharides and/or –OH stretching of carboxylic acid.…”

Section: Resultssupporting

confidence: 89%

“…Two bands at wavenumber 1748 cm −1 and 1712 cm −1 denote –C=O stretching arising from the ester groups of lipids and fatty acids (-C=O in COOH), and bands at wavenumber 1396 cm −1 signify C-H and OH deformation vibrations of carbohydrates that may be arising due to the presence of rhamnose units in the biosurfactants [ 61 ]. A small peak at wavenumber 2358 cm −1 is likely due to the presence of phosphines (P-H 3 ) in the biosurfactant [ 59 ]. The range of spectra below 1000 cm −1 represents C-H, C-O and CH 3 vibrations [ 62 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Mishra

Fatima

Egamberdieva

et al. 2020

Plants

View full text Add to dashboard Cite

In this study, Pseudomonas putida BSP9 isolated from rhizosphere of Brassica juncea was investigated for its plant growth promoting and biosurfactant producing activities. The isolate showed the ability to produce indole acetic acid, siderophore, phosphate solubilization activity and was an efficient producer of biosurfactant. Purification (of the biosurfactant) by thin layer chromatography (TLC) and further characterization by Fourier transform infrared spectroscopy (FTIR) revealed that biosurfactant produced by the isolate belonged to the glycolipid category, which is largely produced by Pseudomonas sp. In addition, liquid chromatography-mass spectroscopy (LC-MS) analysis showed the presence of a mixture of six mono-rhamnolipidic and a di-rhamnolipidic congeners, confirming it as a rhamnolipid biosurfactant. Bioformulations were developed using BSP9 and its biosurfactant to check their impact on promoting plant growth in B. juncea. It was noted from the study that bioformulations amended with biosurfactant (singly or in combination with BSP9) resulted in enhancement in the growth parameters of B. juncea as compared to untreated control. Maximum increment was achieved by plants inoculated with bioformulation that had BSP9 plus biosurfactant. The study also suggested that growth promotion was significant up to a threshold level of biosurfactant and that further increasing the concentration did not further enhance the growth parameter values of the plant. The study proves that novel bioformulations can be developed by integrating plant growth promoting rhizobacteria (PGPR) and their biosurfactant, and they can be effectively used for increasing agricultural productivity while minimizing our dependence on agrochemicals.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Mishra

Fatima

Egamberdieva

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Emulsion layers formed by B14 BS obtained when cells are grown on an optimum culture medium, were stable under a wide range of pH (2–10), and temperature (30–37°C). This result was in agreement with the glycolipid derived from Pseudomonas otitidis , isolated from a coal mine, whose E24 could still be detected when exposed to pH 3–11, and the temperature was as high as 80–100°C ( Singh and Tiwary 2016 ). The stability studies on glycolipid BS derived from P. aeruginosa HAK01 indicated that this BS was able to function under a wide range of temperatures (40–121°C) and pH (4–10) ( Khademolhosseini et al 2019 ).…”

Section: Discussionsupporting

confidence: 87%

“…In order to investigate our glycolipid potential application, antimicrobial activity of cell-free broth containing B14 BS derived from cultures grown on an optimized medium was tested. Although biosurfactant will typically be used in actual application in the form of pure compounds, the cell-free supernatant could be used as a pre-screening process for stability studies and antimicrobial tests ( Singh and Tiwary 2016 ; Mouafo et al 2018 ). At this stage, we then selected to use the cell-free supernatant from strain B14 for these studies, as it is also less time-consuming.…”

Section: Discussionmentioning

confidence: 99%

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Ekprasert

Kanakai

Yosprasong

2020

Polish Journal of Microbiology

View full text Add to dashboard Cite

This work aimed to optimize carbon and nitrogen sources for the growth of Enterobacter cloacae B14 and its biosurfactant (BS) production via One-Variable-At-a-Time (OVAT) method. The BS stability under a range of pH and temperatures was assessed. Antimicrobial activity against Gram-positive and Gram-negative pathogens was determined by the agar well diffusion method. The results showed that the optimum carbon and nitrogen sources for BS production were maltose and yeast extract, respectively, with a maximum BS yield of (39.8 ± 5.2) mg BS/g biomass. The highest emulsification activity (E24) was 79%, which is significantly higher than in the previous studies. We found that B14 BS can withstand a wide range of pH values from 2 to10. It could also function under a range of temperatures from 30–37°C. Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectrometry (FTIR) analysis confirmed that B14 BS is a glycolipid-like compound, which is rarely found in Enterobacter spp. Cell-free broth showed inhibition against various pathogens, preferable to Gram-positive ones. It had better antimicrobial activity against Bacillus subtilis than a commonly-used antibiotic, tetracycline. Furthermore, B14 broth could inhibit the growth of a tetracycline-resistant Serratia marcescens. Our results showed promising B14 BS applications not only for bioremediation but also for the production of antimicrobial products.

show abstract

“…TMB1 has utilized NaNO 3 as the best nitrogen source for maximum growth and production of biosurfactant at optimum temperature (30 °C) and pH (7.2). Singh and Tiwary reported yeast extract as the best nitrogen source for the yield of biosurfactants by Pseudomonas otitidis P4 strain . But for Achromobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Optimization and characterization of a glycolipid produced by Achromobacter sp. to use in petroleum industries

Haloi

Medhi

2019

J Basic Microbiol

View full text Add to dashboard Cite

A biosurfactant, produced by Achromobacter sp. TMB1 is reported through this investigation having physiochemical properties useful for operational and remedial activities in petroleum industries. The strain isolated from soils of local petrol pumps when allowed to grow in glucose containing mineral salts media (MSM) in ambient environment that is, 30°C and varying pH in between 5.5 and 7.2, showed surface tension reduction as low as 34.25 dyne cm −1 at an interval of 48-72 h. Taguchi experiment confirmed the contribution of glucose, yeast extracts, and NaNO 3 concentration on biosurfactant production. FTIR and 1 H NMR analysis of culture supernatant obtained after acid precipitation and solvent extraction methods revealed the glycolipid nature of the extracted compounds. HR-LCMS study further revealed the presence of 10 different types of mono-and di-rhamnolipids congeners with the fatty acids carbon length C 8-12 . The isolated biosurfactant shows functional stability in the temperature range 20-100°C and pH range 2-12 while maintaining the structural integrity till 550°C (TGA-DSC), strongly suggests its potential applications in petroleum industries. K E Y W O R D SAchromobacter sp. TMB1, MEOR and bioremediation, rhamnolipids, surface tension

show abstract

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Cited by 69 publications

References 71 publications

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Optimization and characterization of a glycolipid produced by Achromobacter sp. to use in petroleum industries

Contact Info

Product

Resources

About