Physicochemical properties of ionic and non-ionic biocompatible hydrogels in water and cell culture conditions: Relation with type of morphologies of bovine fetal fibroblasts in contact with the surfaces

Rivero, Rebeca Edith; Alustiza, Fabrisio; Capella, Virginia; Liaudat, Cecilia; Rodríguez, Nancy; Bosch, Pablo; Barbero, C.; Rivarola, Claudia R.

doi:10.1016/j.colsurfb.2017.07.032

Cited by 33 publications

(48 citation statements)

References 35 publications

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“…Based on our present and previous results [17,19,26,29] we think that the moderate hydrophilic surfaces will be the most useful for skin substitution. Also as reported by Rivero et al [30] the moderate hydrophilic surfaces are favorable for cell adhesion and proliferation.…”

Section: Discussionsupporting

confidence: 59%

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Ładniak

Jurak

Wiącek

2019

Colloids and Interfaces

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The investigations were carried out to determine wettability of the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers transferred from the liquid subphases containing chitosan (Ch), hyaluronic acid (HA), and/or titanium dioxide (TiO2) to a glass support by means of the Langmuir–Blodgett (LB) technique. For comparative purposes, the analysis of the plates surfaces emerged from the analogous subphases without the phospholipid film was also made. Characterization of the DPPC monolayers was based on the contact angle measurements using three test liquids (water, formamide, diiodomethane) and a simulated body fluid (SBF) solution in which the concentration of ions was close to that of human plasma. After deposition of the DPPC monolayers on the glass plates, a significant increase in the contact angles of all the probe liquids was observed compared to the plates pulled out from the given subphase without floating DPPC. The presence of phospholipid monolayer increased the hydrophobic character of the surface due to orientation of its molecules with hydrocarbon chains towards the air. In addition, the components of the subphase attached along with DPPC to the glass support modify the surface polarity. The largest changes were observed in the presence of TiO2.

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Section: Discussionsupporting

confidence: 59%

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Ładniak

Jurak

Wiącek

2019

Colloids and Interfaces

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“…It was also demonstrated that the PNIPAM surfaces are biocompatible in contact with bovine fetal broblast, showing high cellular adhesion and proliferation aer 15 days of culture. 12,25,39 Herein, we demonstrate that the use of this material as a 3D structure for cell growth is also possible. Evidently, the 3D macroporous hydrogel is cytocompatible.…”

Section: D Cell Proliferation By Confocal Laser Scanning Microscopymentioning

confidence: 82%

“…For example, the % Sw(eq) of non-porous (molecular porosity) PNIPAM was reported as 2600% in water and 941% in DMEM culture medium at 25 C, while at 37 C (culture condition), the % Sw(eq) decreases to 89% in both the mediums. 12 On the other hand, the % Sw(eq) of the macroporous PNIPAM hydrogel in water is 1900% at 25 C, 25 which is rather less than that in the non-porous PNIPAM. The swelling capacity must not only be strongly dependent on the solvent and environment temperature but also dependent of the matrix morphology.…”

Section: Physicochemical and Mechanical Properties Of The 3d Macropormentioning

confidence: 99%

“…On the next day, BFFs were seeded onto macroporous hydrogels at a density of 10 5 cells per well, as shown in Scheme 3, and incubated at 37 C under 5% CO 2 humidied atmosphere for 75 days. 12 Viability assays. To evaluate the material cytotoxicity, two assays were carried out-MTT and Neutral Red uptake (NR).…”

Section: Biological Experimentsmentioning

confidence: 99%

“…PNIPAM is one of the most widely used synthetic polymers for biomedical applications due to its volume phase transition temperature of about 32 C, which is close to the normal human body temperature, and its proven biocompatibility. [7][8][9][10][11] The biocompatibility of PNIPAM in contact with BFFs, 12 human epithelial colorectal adenocarcinoma cells (Caco-2) and lung cancer cells (Calu-3), 13 mesenchymal stem cells derived from rat bone marrow (BM-MSCs), human adipose tissue (AT-MSCs), 14 smooth muscle (SMC), 15 and other biological systems has been demonstrated. In addition, the cytotoxicity of PNIPAM and bioadhesive properties of murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293), and human carcinoma-derived cells (A549) in contact with these surfaces were recently studied.…”

Section: Introductionmentioning

confidence: 99%

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Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation

et al. 2020

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Interaction Between Hyaluronic Acid Semi‐Interpenetrated Hydrogel with Bull Spermatozoa: Studies of Sperm Attachment–Release and Sperm Quality

Blois

Liaudat

Capella

et al. 2021

Adv Materials Inter

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The study of living systems in contact with biocompatible synthetic hydrogels is developing. The authors’ group is the first to propose use of polymeric materials to accomplish sperm selection process by attachment of bull sperm to hydrogel surfaces, this being a crucial step for successful assisted reproductive techniques (ARTs). Surfaces based on poly(N‐isopropylacrylamide) hydrogels (PNIPAM) and ionic and neutral copolymers are synthetized. Their physicochemical properties in aqueous and culture medium are related to the percentage of attachment and subsequent release of bull spermatozoa from the hydrogel surface. High attaching capacity (30–45%) of sperm on cationic and neutral surfaces is mainly observed, however, sperm detachment from the hydrogel using traditional induction processes (i.e., sperm capacitation) is not significant. Therefore, PNIPAM‐co‐20%‐N‐Tris(hydroxymethyl)methyl acrylamide (PNIPAM‐HMA) hydrogel surface is semi‐interpenetrated with hyaluronic acid (HA). In this case, 50% of spermatozoa are attached to PNIPAM‐HMA‐HA hydrogel and after treatment with hyaluronidase, 47% of them are released. Collected sperm show acceptable characteristics of progressive motility (70% with score 4 for vigor), high viability, and cytoplasmic membrane integrity. Noteworthy, the study of the interaction of hydrogel surface/spermatozoa can be extended to human and other mammalian species, in order to provide advanced alternatives for gamete selection for ARTs.

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Physicochemical properties of ionic and non-ionic biocompatible hydrogels in water and cell culture conditions: Relation with type of morphologies of bovine fetal fibroblasts in contact with the surfaces

Cited by 33 publications

References 35 publications

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Mechanical and physicochemical behavior of a 3D hydrogel scaffold during cell growth and proliferation

Interaction Between Hyaluronic Acid Semi‐Interpenetrated Hydrogel with Bull Spermatozoa: Studies of Sperm Attachment–Release and Sperm Quality

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