Physicochemical properties of intact fungal cell wall determine vesicles release and nanoparticles internalization

Ebrahimi, Hoda; Siavoshi, Farideh; Jazayeri, Mir Hadi; Sarrafnejad, Abdolfattah; Saniee, Parastoo; Mobini, Maryam

doi:10.1016/j.heliyon.2023.e13834

Cited by 6 publications

(2 citation statements)

References 49 publications

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“…The internalization routes of RIPs have not been studied in fungi, but, given that they also possess endocytic mechanisms similar to those of animal cells [ 70 , 71 ], it would be expected that the routes of access to ribosomes would be similar to those discovered in animal cells. In addition, the cell wall represents an important barrier to the passage of macromolecules [ 72 ], which, given the diversity of the composition and structure of the cell walls of different fungi [ 73 ], could be an important element explaining, in part, the different sensitivities of fungi to different RIPs.…”

Section: Endocytosis Of Ribosome-inactivating Proteinsmentioning

confidence: 99%

Antifungal Activity of Ribosome-Inactivating Proteins

Iglesias,

Citores,

Gay

et al. 2024

Toxins

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The control of crop diseases caused by fungi remains a major problem and there is a need to find effective fungicides that are environmentally friendly. Plants are an excellent source for this purpose because they have developed defense mechanisms to cope with fungal infections. Among the plant proteins that play a role in defense are ribosome-inactivating proteins (RIPs), enzymes obtained mainly from angiosperms that, in addition to inactivating ribosomes, have been studied as antiviral, fungicidal, and insecticidal proteins. In this review, we summarize and discuss the potential use of RIPs (and other proteins with similar activity) as antifungal agents, with special emphasis on RIP/fungus specificity, possible mechanisms of antifungal action, and the use of RIP genes to obtain fungus-resistant transgenic plants. It also highlights the fact that these proteins also have antiviral and insecticidal activity, which makes them very versatile tools for crop protection.

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Section: Endocytosis Of Ribosome-inactivating Proteinsmentioning

confidence: 99%

Antifungal Activity of Ribosome-Inactivating Proteins

Iglesias,

Citores,

Gay

et al. 2024

Toxins

View full text Add to dashboard Cite

show abstract

“…It is widely known that yeast cell surface is negatively charged, porous and viscoelastic in nature [41]. Due to anionicness and nanoform, the cationic SNPs caused destructive morphological changes.…”

Section: Snps Induced Alterations In the Morphology Of Fungal Cellsmentioning

confidence: 99%

Unveiling the role of sulfur nanoparticles as an anti-dermatomycotic agent against Pichia kudriavzevii

Biswas,

Mukherjee,

Sarkar

et al. 2023

Preprint

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Non-metal nanoparticles are now a days widely applied in different fields. Among various non-metal nanoparticles (NPs), sulfur nanoparticles (SNPs) are one of the most significant and emerging nanoparticles. In this study, sulfur nanoparticles (SNPs) were prepared by eco-friendly method and well characterized by UV-visible spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. The prepared nanoparticles were spherical in shape with mean size of 35 nm and positive surface charge. Here, an attempt has been made to check the in vitro efficacy of sulfur nanoparticles against the dermatomycosis causing fungus Pichia kudriavzevii. Different antimicrobial experiments were performed to check the potential of SNPs against Pichia kudriavzevii. SEM micrographs and growth inhibition assay confirmed the efficacy of SNPs against the studied fungus. The antifungal activity of SNPs evaluated through the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC). The values of MIC were 50µM SNPs for the studied species, and MFC was 250µM for Pichia kudriavzevii. Treatment with SNPs was found to be effective in altering the cell morphology, inhibiting normal growth, and reducing the cell membrane content, mainly ergosterol of Pichia kudriavzevii. The status of present study can further be use to unravel the detailed mechanism of antifungal activity of sulfur nanoparticles against Pichia kudriavzevii for future workers.

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Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications

Cheng,

Kwon,

Adeshara

et al. 2023

Appl Microbiol Biotechnol

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Extracellular RNAs are an emerging research topic in fungal-plant interactions. Fungal plant pathogens and symbionts release small RNAs that enter host cells to manipulate plant physiology and immunity. This communication via extracellular RNAs between fungi and plants is bidirectional. On the one hand, plants release RNAs encapsulated inside extracellular vesicles as a defense response as well as for intercellular and inter-organismal communication. On the other hand, recent reports suggest that also full-length mRNAs are transported within fungal EVs into plants, and these fungal mRNAs might get translated inside host cells. In this review article, we summarize the current views and fundamental concepts of extracellular RNAs released by plant-associated fungi, and we discuss new strategies to apply extracellular RNAs in crop protection against fungal pathogens. Key points • Extracellular RNAs are an emerging topic in plant-fungal communication. • Fungi utilize RNAs to manipulate host plants for colonization. • Extracellular RNAs can be engineered to protect plants against fungal pathogens.

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Physicochemical properties of intact fungal cell wall determine vesicles release and nanoparticles internalization

Cited by 6 publications

References 49 publications

Antifungal Activity of Ribosome-Inactivating Proteins

Antifungal Activity of Ribosome-Inactivating Proteins

Unveiling the role of sulfur nanoparticles as an anti-dermatomycotic agent against Pichia kudriavzevii

Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications

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