Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications

Cheng, An-Po; Kwon, Seomun; Adeshara, Trusha; Göhre, Vera; Feldbrügge, Michael; Weiberg, Arne

doi:10.1007/s00253-023-12718-7

Cited by 5 publications

(4 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent findings confirmed that bacterial or fungal infection boosts the secretion of plant EVs containing diverse defence-related proteins, sRNAs, and lipid signals, suggesting their involvement in plant defence mechanisms [ 83 , 84 ]. For instance, a study involving Arabidopsis being infected with the powdery mildew Golovinomyces orontii demonstrated that plant-derived exosome-like vesicles transport both PEN1/SYP121 and the ABC transporter Penetration 3 (PEN3), which accumulate in the haustorial encasements, creating a defence barrier that restricts fungal entry.…”

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 94%

“…However, one of the most intriguing aspects, perhaps yet to be unravelled, is the role of EVs in the mutualistic and symbiotic relationships between organisms. Even if the role of the plant endomembrane system in EV biogenesis, trafficking, and uptake has been recently reviewed, there are still missing data about the real role of EVs during arbuscular mycorrhizal symbiosis [ 84 ]. A recent ultrastructural analysis documented the presence of EVs within the peri-arbuscular interface during the formation and maturation of Rhizophagus irregularis arbuscules in rice ( Oryza sativa ) [ 85 ].…”

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 99%

“…A recent ultrastructural analysis documented the presence of EVs within the peri-arbuscular interface during the formation and maturation of Rhizophagus irregularis arbuscules in rice ( Oryza sativa ) [ 85 ]. Recently, common bean ( Phaseolus vulgaris ) PvTET8.1 , the homologue of the A. thaliana exosomal marker TET8, was found to be upregulated during arbuscular mycorrhizal fungi and rhizobia association [ 84 ]. Moreover, the silencing of pvTET8.1 reduces the size and the number of nodules, nitrogen fixation, and mycorrhizal arbuscules.…”

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 99%

See 2 more Smart Citations

Plant Extracellular Vesicles: Current Landscape and Future Directions

Ambrosone,

Barbulova,

Cappetta

et al. 2023

Plants

View full text Add to dashboard Cite

Plant cells secrete membrane-enclosed micrometer- and nanometer-sized vesicles that, similarly to the extracellular vesicles (EVs) released by mammalian or bacterial cells, carry a complex molecular cargo of proteins, nucleic acids, lipids, and primary and secondary metabolites. While it is technically complicated to isolate EVs from whole plants or their tissues, in vitro plant cell cultures provide excellent model systems for their study. Plant EVs have been isolated from the conditioned culture media of plant cell, pollen, hairy root, and protoplast cultures, and recent studies have gathered important structural and biological data that provide a framework to decipher their physiological roles and unveil previously unacknowledged links to their diverse biological functions. The primary function of plant EVs seems to be in the secretion that underlies cell growth and morphogenesis, cell wall composition, and cell–cell communication processes. Besides their physiological functions, plant EVs may participate in defence mechanisms against different plant pathogens, including fungi, viruses, and bacteria. Whereas edible and medicinal-plant-derived nanovesicles isolated from homogenised plant materials ex vivo are widely studied and exploited, today, plant EV research is still in its infancy. This review, for the first time, highlights the different in vitro sources that have been used to isolate plant EVs, together with the structural and biological studies that investigate the molecular cargo, and pinpoints the possible role of plant EVs as mediators in plant–pathogen interactions, which may contribute to opening up new scenarios for agricultural applications, biotechnology, and innovative strategies for plant disease management.

show abstract

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 94%

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 99%

Section: Plant Ev–microorganism Interactionsmentioning

confidence: 99%

See 1 more Smart Citation

Plant Extracellular Vesicles: Current Landscape and Future Directions

Ambrosone,

Barbulova,

Cappetta

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…How such HGT occurs from a non-plant organism to a phytovirus is unclear. Recent studies have evidenced the transport of mRNA from fungal pathogens to plants(Cheng et al, 2023), and this could hypothetically lead to an RNA-RNA recombination with a replicating potyviral/secoviral virus. In the potyviral genomes, the region corresponding to the ITPase gene insertion corresponds to the Nlb-CP junction that is a known mutational hotspot(Nigam et al, 2019), which may have favored such recombination.In contrast to MMV and MSV1, no ITPase was detected in the dsRNA genome of MPV1.…”

mentioning

confidence: 99%

A mixed infection of ITPase-encoding potyvirid and secovirid in Mercurialis perennis: evidences for a convergent euphorbia-specific viral counterstrike

Mahillon,

Brodard,

Dubuis

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: In cellular organisms, inosine triphosphate pyrophosphatases (ITPases) prevent the incorporation of mutagenic deaminated purines into nucleic acids. These enzymes have also been detected in the genomes of several plant RNA viruses infecting two euphorbia species. In particular, two ipomoviruses produce replicase-associated ITPases to cope with high concentration of non-canonical nucleotides found in cassava tissues. Method: Using high-throughput RNA sequencing on the wild euphorbia species Mercurialis perennis, two new members of the families Potyviridae and Secoviridaewere identified. Both viruses encode for a putative ITPase, and were found in mixed infection with a new partitivirid. Following biological and genomic characterization of these viruses, the origin and function of the newly-identified phytoviral ITPases were investigated. Results: While the potyvirid was shown to be pathogenic, the secovirid and partitivirid could not be transmitted. The secovirid was found belonging to a proposed new Comovirinaegenus tentatively named "Mercomovirus", which also accommodates other viruses identified through transcriptome mining, and for which an asymptomatic pollen-associated lifestyle is suspected. Homology and phylogenetic analyses inferred that the ITPases encoded by the potyvirid and secovirid were likely acquired through independent horizontal gene transfer events, forming lineages distinct from the enzymes found in cassava ipomoviruses. Possible origins from cellular organisms are discussed for these proteins. Strikingly, the endogenous ITPase of M. perennis is predicted to encode for a C-terminal nuclear localization signal, which appears to be conserved among the ITPases of euphorbias but absent in other plant families. This particular subcellular localization is in line with the idea that the plant nucleic acids remain protected in the nucleus, while deaminated nucleotides accumulate in the cytoplasm where they act as antiviral molecules. Conclusion: Three new RNA viruses infecting M. perennis are described, two of which encoding for ITPases. These enzymes have distinct origins, and are likely required by viruses to circumvent high level of cytoplasmic non-canonical nucleotides. This putative plant defense mechanism has emerged early in the evolution of euphorbias, and seems to specifically target certain groups of RNA viruses infecting perennial hosts.

show abstract