Physicochemical properties of chitosan-coated nanoliposome loaded with orange seed protein hydrolysate

Mazloomi, Seyadeh Narges; Mahoonak, Alireza Sadeghi; Ghorbani, Mohammad; Houshmand, Gholamreza

doi:10.1016/j.jfoodeng.2020.109976

Cited by 42 publications

(25 citation statements)

References 32 publications

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“…Identification of the origin of protein for peptides was done by using the UniProt database and NCBInr database. The BIOPEP-UWM database was used to search for similarities between the sequences identified in this study and previously published bioactive peptide sequences [15,29,30]. Only identification results showing a confidence higher of 90% were reported.…”

Section: Identification Of Peptides Using Mass Spectrometry In Tandemmentioning

confidence: 99%

“…The wastes of the juice industry, including peels, seeds, and pulps, represent about 50% of the raw processed fruit. This potentially valuable resource, otherwise processed, can aggravate disposal problems [15]. Orange seed flour, having been defatted, is considered a by-product containing about 17.9-26.5% of protein that is possible to be implemented as a valuable and economical source for proteins extraction and hydrolysis [16].…”

Section: Introductionmentioning

confidence: 99%

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Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products

Mazloomi

Mora

Aristoy

et al. 2020

Foods

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In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1–3% (v/w) and hydrolysis times (2–5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p < 0.05) were achieved by using a hydrolysis time of 5 h and an enzyme concentration of 2%. Orange seed protein hydrolysate (OSPH) was prepared under these conditions, and peptides were isolated and purified by using size-exclusion chromatography and high-performance liquid chromatography, respectively. The fractions that showed the highest biological activities were analyzed by mass spectrometry in tandem, and a total of 63 peptide sequences were found. Moreover, the effect of simulated gastrointestinal digestion on the bioactivity of the fractions was studied, and the novel peptide sequences generated were also identified. Overall, despite there being some differences in the profile of peptide sequences obtained, the main results showed non-significant differences in the analyzed bioactivities after simulated gastrointestinal digestion.

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Section: Identification Of Peptides Using Mass Spectrometry In Tandemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products

Mazloomi

Mora

Aristoy

et al. 2020

Foods

Self Cite

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“…Entrapment efficiency percentage (EE%) is an important index to determine the capability of nanosystems in preserving and stabilizing the encapsulated compounds [ 41 ]. In this study, the EE% test revealed that the set of OEO-loaded nanoliposomes reached values up to 79.55 ± 6.9% (see Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

et al. 2021

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The development of greener nano-constructs with noteworthy biological activity is of supreme interest, as a robust choice to minimize the extensive use of synthetic drugs. Essential oils (EOs) and their constituents offer medicinal potentialities because of their extensive biological activity, including the inhibition of fungi species. However, their application as natural antifungal agents are limited due to their volatility, low stability, and restricted administration routes. Nanotechnology is receiving particular attention to overcome the drawbacks of EOs such as volatility, degradation, and high sensitivity to environmental/external factors. For the aforementioned reasons, nanoencapsulation of bioactive compounds, for instance, EOs, facilitates protection and controlled-release attributes. Nanoliposomes are bilayer vesicles, at nanoscale, composed of phospholipids, and can encapsulate hydrophilic and hydrophobic compounds. Considering the above critiques, herein, we report the in-house fabrication and nano-size characterization of bioactive oregano essential oil (Origanum vulgare L.) (OEO) molecules loaded with small unilamellar vesicles (SUV) nanoliposomes. The study was focused on three main points: (1) multi-compositional fabrication nanoliposomes using a thin film hydration–sonication method; (2) nano-size characterization using various analytical and imaging techniques; and (3) antifungal efficacy of as-developed OEO nanoliposomes against Trichophyton rubrum (T. rubrum) by performing the mycelial growth inhibition test (MGI). The mean size of the nanoliposomes was around 77.46 ± 0.66 nm and 110.4 ± 0.98 nm, polydispersity index (PdI) of 0.413 ± 0.015, zeta potential values up to −36.94 ± 0.36 mV were obtained by dynamic light scattering (DLS). and spherical morphology was confirmed by scanning electron microscopy (SEM). The presence of OEO into nanoliposomes was displayed by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy. Entrapment efficiency values of 79.55 ± 6.9% were achieved for OEO nanoliposomes. In vitro antifungal activity of nanoliposomes tested against T. rubrum strains revealed that OEO nanoliposomes exhibited the highest MGI, 81.66 ± 0.86%, at a concentration of 1.5 µL/mL compared to the rest of the formulations. In summary, this work showed that bioactive OEO molecules with loaded nanoliposomes could be used as natural antifungal agents for therapeutical purposes against T. rubrum.

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“…As a result of its permeation-enhancing effects, chitosan-based systems have been recognized as among the most promising matrices for bioactive peptide entrapment and delivery. Thus, these systems have been recently proven to increase the in vivo antihypertensive efficacy of stone fish protein hydrolyzates [ 229 , 230 ] and to retain the antioxidant activity of rainbow trout protein [ 232 ] and seed protein [ 245 ] hydrolyzates. Additionally, tripolyphosphate (TPP) is the most accepted crosslinker to prepare chitosan hydrogels through ionic crosslinking [ 249 , 250 ].…”

Section: Bioavailability Of Food Peptidesmentioning

confidence: 99%

Current Evidence on the Bioavailability of Food Bioactive Peptides

2020

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Food protein-derived bioactive peptides are recognized as valuable ingredients of functional foods and/or nutraceuticals to promote health and reduce the risk of chronic diseases. However, although peptides have been demonstrated to exert multiple benefits by biochemical assays, cell culture, and animal models, the ability to translate the new findings into practical or commercial uses remains delayed. This fact is mainly due to the lack of correlation of in vitro findings with in vivo functions of peptides because of their low bioavailability. Once ingested, peptides need to resist the action of digestive enzymes during their transit through the gastrointestinal tract and cross the intestinal epithelial barrier to reach the target organs in an intact and active form to exert their health-promoting properties. Thus, for a better understanding of the in vivo physiological effects of food bioactive peptides, extensive research studies on their gastrointestinal stability and transport are needed. This review summarizes the most current evidence on those factors affecting the digestive and absorptive processes of food bioactive peptides, the recently designed models mimicking the gastrointestinal environment, as well as the novel strategies developed and currently applied to enhance the absorption and bioavailability of peptides.

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Physicochemical properties of chitosan-coated nanoliposome loaded with orange seed protein hydrolysate

Cited by 42 publications

References 32 publications

Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products

Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products

Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities

Current Evidence on the Bioavailability of Food Bioactive Peptides

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