Physicochemical Properties and Field Evaluation of Monolithic Wax Formulations for the Controlled Release of a Forest Pest Pheromone

Slodowicz, Mariel; Ceriani-Nakamurakare, Esteban; Carmarán, Cecilia Cristina; González-Audino, Paola

doi:10.5935/0103-5053.20160184

Cited by 2 publications

(2 citation statements)

References 17 publications

(19 reference statements)

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“…[72] For natural waxes, in particular, a volatile release retardation was found by others and explained by the formation of closed spaces within the formulation matrix that reduce the mobility of molecules within the crystalline network and by reduced solubility of the encapsulated molecule. [36,56,73] Atterholt et al [74] found soy oil as a volatility suppressant of pheromone release from paraffin wax-based emulsion. They suggest an increased solubility of the pheromone in the paraffin matrix by the soy oil, causing a decrease in the release rate.…”

Section: Candelilla Wax Slows 𝜷-Caryophyllene Releasementioning

confidence: 99%

Development of a Self‐Adhesive Oleogel Formulation Designed for the Slow Release of Semiochemicals

Muskat

Brikmann

et al. 2022

Macro Materials & Eng

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Oleogels have been widely studied as delivery matrices for lipophilic compounds. Despite their potential, oleogels are underexplored for the formulation and release of semiochemicals for insect behavior manipulation strategies. An ethylcellulose-candelilla wax-oleogel as a suitable carrier for the slow release of volatile semiochemicals, employing the sesquiterpene 𝜷-caryophyllene as a model substance is presented. A current drawback of ethylcellulose oleogels is the high temperature needed during the oleogel preparation procedure, leading to excessive volatilization of the active compounds. Reducing the gelling temperature from 104.8 to 66.9 °C and, thus, the volatile addition temperature by addition of the softener octyldodecanol results in 95.28% entrapment efficiency. Experimental results demonstrate release modifiability by tailoring the matrix crystalline state due to candelilla wax addition. Furthermore, a method is established for analyzing the self-adhesive properties of the gel on plant leaves. In addition, the gel's self-adhesive properties employed on apple leaves are synergistically affected by oil leakage and gel stiffness. Olfactometer experiments showed that the formulation is able to enhance the attractiveness of aphid-infested plants for parasitic wasps. The present study provides a novel matrix-type semiochemical formulation based on non-toxic, biobased and biodegradable materials and will contribute to the establishment of oleogels as delivery systems for semiochemicals.

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Section: Candelilla Wax Slows 𝜷-Caryophyllene Releasementioning

confidence: 99%

Development of a Self‐Adhesive Oleogel Formulation Designed for the Slow Release of Semiochemicals

Muskat

Brikmann

et al. 2022

Macro Materials & Eng

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“…These compounds are attractive to females and are involved in the mating process of this beetle. They have been used successfully in mating disruption trials for the management of this beetle in poplar and hazelnut plantations (Funes et al., , ; Slodowicz et al., ).…”

Section: Introductionmentioning

confidence: 99%

Sex pheromone component produced by microbial associates of the forest pest Megaplatypus mutatus

Slodowicz¹,

Ceriani-Nakamurakare

Carmarán

et al. 2019

Entomologia Exp Applicata

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Megaplatypus mutatus (Chapuis) (Coleoptera: Platypodidae) is an ambrosia beetle native to South America that causes economic loss and was recently introduced to Italy, where it attacks and damages live poplar trees. Sulcatol and sulcatone are male‐produced pheromone components involved in the mating process of M. mutatus. Their relative proportions are highly variable among insects, although the temporal pattern shows that initially only sulcatol is present, and sulcatone increases with time, until they are finally both depleted. Sulcatol and sulcatone may be produced de novo by the beetles, they may be produced by fungi, or both pathways may contribute to their production. Sulcatol is stored in the males’ hindgut but sulcatone is only present in emissions, so there is an oxidation process to transform the alcohol to the ketone before or during pheromone release. It is our hypothesis that fungi associated with M. mutatus are responsible for this process. In this work, we studied a possible contribution of associated microorganisms in the conversion of sulcatol into sulcatone and its consequent role in the temporal release pattern of these sex pheromone components observed in male insects. Moreover, we inhibited the postulated enzymes involved in this pheromone conversion process – 3‐hydroxy‐3‐methyl‐glutatyl‐CoA reductase (HMGR) and P450 enzymes of a fungal strain – and added an antibiotic and a fungicide to the homogenate during sulcatol‐sulcatone conversion. Among the fungal species, particular interest was given to Graphium basitruncatum (Matsush.) Seifert & Okada (Microascales), as it is present in male but not in female exoskeletons and in insect gallery samples, suggesting a possible different role in pherome production, as the male is the pheromone‐producing sex. Several isolated strains were able to convert sulcatol to sulcatone, whereas the fungus G. basitruncatum showed the highest production of this ketone. Additionally, inhibition of P450 enzymes and HMGR from G. basitruncatum on this alcohol‐ketone conversion demonstrated that HMGR is involved in sulcatone generation using sulcatol as precursor, and that P450 enzymes are not. Finally, sulcatone production diminished significantly in homogenized tissues of male and female M. mutatus following addition of an antibiotic and a fungicide. The results suggest that fungi associated with M. mutatus are involved in pheromone production.

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Physicochemical Properties and Field Evaluation of Monolithic Wax Formulations for the Controlled Release of a Forest Pest Pheromone

Cited by 2 publications

References 17 publications

Development of a Self‐Adhesive Oleogel Formulation Designed for the Slow Release of Semiochemicals

Development of a Self‐Adhesive Oleogel Formulation Designed for the Slow Release of Semiochemicals

Sex pheromone component produced by microbial associates of the forest pest Megaplatypus mutatus

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