Physicochemical properties and cryoprotective action of solutions of polyvinyl alcohol, glycerol and media based on their combinations during freezing of red blood cells

Hvozdiuk, Yana; Chekanova, V. V.; Pakhomova, Yulia S.; Kompaniets, A. M.

doi:10.15407/cryo30.03a.288

Cited by 3 publications

(1 citation statement)

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“…З переліку цих сполук особливої уваги заслуговує синтетичний полімер -полівініловий спирт (ПВС) [8,9]. Попередні дослідження співробітників нашого інституту показали, що ПВС утворює гідрофобні домени, завдяки цьому сполука має властивість активного інгібітора рекристалізації льоду, підвищує ефективність заморожування деяких клітин, зокрема -еритроцитів [10,11].…”

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Physicochemical Properties of Solutions of Polyvinyl Alcohol (PVA) of Different Molecular Weights

Chekanova¹,

Pakhomova²,

Kompaniets³

et al. 2021

Ukr. ž. med. bìol. sportu

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The physicochemical properties (surface tension, dynamic viscosity, crystallization and melting temperatures) of polyvinyl alcohol solutions of molecular weight 9, 31 and 72 kDa have been studied. The surface tension and the critical concentration of micelle formation were determined by the method of stalogometry, and the dynamic viscosity was determined using an Oswald viscometer. The crystallization and melting temperatures were determined in a cooled modified chamber of the UOP-6 software freezer at a rate of 2°C/min. Cryomicroscopic studies were carried out on a polarizing microscope "MIN-8". The surface tension reflects the interaction of PVA solutions with the lipid layer of biomembranes and indicates the hydrophobic properties of substances. The viscosity of PVA solutions characterizes their interaction with water molecules and reflects hydrophilic interactions. The purpose of the study is to determine the physicochemical properties of PVS that characterize the hydrophilic-hydrophobic interactions in the studied solutions and the micelle formation of PVА solutions of different molecular weights. Materials and methods. Studies of the dynamic viscosity and density of 0.1%-1% PVA solutions of molecular weight 9, 31 kDa showed that these parameters increase with increasing PVA concentration, which leads to increased hydrophilicity of the solutions. Results and discussion. It was shown that the surface tension of PVA solutions decreases with increasing concentration, which leads to a decrease in the hydrophobic properties of the polymer. It was found that in 0.5% PVА solutions of molecular weight 9 and 31 kDa the crystallization and melting temperatures decrease from -5 to -6°C. At these temperatures, crystallization and melting of the solutions begin. Conclusion. The study of micelle formation in PVА solutions of different molecular masses was carried out, surface tension isotherms were constructed, and the break point on the isotherm corresponding to the CCM was determined. The values of the critical concentration of micelle formation of PVА of molecular masses 9, 31, 72 kDa were determined. Hydrophobic links of PVА of molecular masses 9 and 31 kDa form hydrophobic cavities in the micelle structure, which can reduce recrystallization activity

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Physicochemical Properties of Solutions of Polyvinyl Alcohol (PVA) of Different Molecular Weights

Chekanova¹,

Pakhomova²,

Kompaniets³

et al. 2021

Ukr. ž. med. bìol. sportu

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Prolonged Storage of Thawed Red Blood Cells

Orlyk

Kondratskyi²,

Vynarchyk³

et al. 2022

Proc Shevchenko Sci Soc Med Sci

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Introduction. In modern transfusion practice, both in peacetime and in military conditions, red blood cells (RBCs) are widely used as the main component of donor blood. Cryopreserved red blood cells are considered the most safe and high-quality RBC-containing environment. However, the storage period of thawed RBCs after cryopreservation is limited to 24 hours, and significantly complicates their use. Therefore, extending the storage period of thawed RBCs is relevant for the blood service. Research objective: study the RBCs morphological state and functional completeness that were cryopreserved at -40ºС and stored for 7 days at a temperature of +2ºС - +4ºС after thawing. Materials and methods. The object of the study were RBCs that were cryopreserved at -40ºС and stored for 7 days at a temperature of +2ºС - +4ºС after thawing. Deglycerolization of the thawed red blood cells, cryopreserved at -40ºС, required three time washing by using reverse cytoagglomeration. Thawed RBCs were re-suspended in lactate-sucrose-phosphate solution. After RBC thawing and storage for 7 days (186 doses) in the suspension the following indicators were studied: free hemoglobin, extracellular potassium, adenosine triphosphoric acid (ATP), 2,3-diphosphoglycerate (2,3-DPG), hematocrit, degree of hemoglobin affinity to oxygen (P50,), viscosity coefficient, osmotic stability, electrophoretic mobility of erythrocytes. as well as the total number of cells lost and recovered. Results. After storage for 7 days of suspension of thawed RBCs at a temperature of +2ºС - +4ºС indicators of free hemoglobin (0,62±0,02 g/l), extracellular potassium (2,7±0,3 mmol/l), hematocrit (0,4±0,02 l/l) were within normal limits. Osmotic resistance (0,46±0,02%), electrophoretic mobility (0,94±0,04 µm·cm·V-1·s-1) of RBCs, suspension viscosity factor (5,5±0,20mPa·С) did not exhibit changes in comparison with normal values. High levels of ATP indicators (3,0±0,2 µmol/gHb) and 2,3-DPG (10,5±1,3 µmol/gHb) were established. Indicator Р50 (24,1±1,3 hPa) corresponded to low hemoglobine affinity for oxygen. After 7-day storage at +2ºС - +4ºС total cell loss was insignificant and amounted to 5,6±0,4%. High percentage of viable thawed RBCs 94,4±0,5% was shown. Conclusions. Deglycerolization of thawed red blood cells, cryopreserved at -40ºС, by reverse cytoagglomeration, as well as use of lactate-sucrose-phosphate solution for washed RBCs resuspending promote prolongation of thawed RBCs storage period up to 7 days at +2ºС - +4ºС in viable condition.

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RESEARCH IN COMPARATIVE ASPECT OF CRYOPROTECTIVE ACTION OF GLYCERIN-BASED CRYOPRESERVATIVES IN COMBINATION WITH POLIVINYL ALCOHOL OF DIFFERENT MOLECULAR WEIGHTS AT FAST AND SLOW COOLING OF HUMAdN DONOR BLOOD

Hvozdiuk

Kompaniets

2022

VPBM

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Physicochemical properties and cryoprotective action of solutions of polyvinyl alcohol, glycerol and media based on their combinations during freezing of red blood cells

Cited by 3 publications

References 0 publications

Physicochemical Properties of Solutions of Polyvinyl Alcohol (PVA) of Different Molecular Weights

Physicochemical Properties of Solutions of Polyvinyl Alcohol (PVA) of Different Molecular Weights

Prolonged Storage of Thawed Red Blood Cells

RESEARCH IN COMPARATIVE ASPECT OF CRYOPROTECTIVE ACTION OF GLYCERIN-BASED CRYOPRESERVATIVES IN COMBINATION WITH POLIVINYL ALCOHOL OF DIFFERENT MOLECULAR WEIGHTS AT FAST AND SLOW COOLING OF HUMAdN DONOR BLOOD

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