Physicochemical properties and characterization of chitosan synthesized from fish scales, crab and shrimp shells

Kumari, Suneeta; Annamareddy, Sri Hari Kumar; Sahoo, Abanti; Rath, Prasenjit

doi:10.1016/j.ijbiomac.2017.04.119

Cited by 261 publications

(153 citation statements)

References 36 publications

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“…The demineralization process to remove inorganic salts or minerals in the crab carapace. The content of noble minerals is CaCO3 and Ca3(PO4)2 in small quantities, the minerals contained in this carapace are easier compared to proteins because they are only physically bound (Kumari et al, 2017). Demineralization is generally performed by acid treatment using HCl, HNO3, H2SO4, CH3COOH, and HCOOH (No and Hur, 1998;Percot et al, 2003).…”

Section: Isolation Of Chitinmentioning

confidence: 99%

Potential of Chitosan From Local Crab (Portunus Pelagicus) to Enhance Storability of Musa Paradisiaca L.

Buanasari¹,

Sugiyo²,

Fitriani³

et al. 2019

JBAT

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Potential waste of local crab carapace (Portunus pelagicus) as a source of chitosan as an active layer that can protect bananas has been studied. The process in this study consists of three stages. The first stage was the isolation of chitin through deproteinization process using 2.0 N NaOH solution with a ratio of 1:6 w/v and demineralization process using 1.5 N HCl solution with a ratio of 1:12 w/v. The second stage is the deacetylation stage using 50% NaOH solution with a ratio of 1:20 w/v. Fourier Transform Infra-Red (FTIR) Spectroscopy is used to determine the degree of deacetylation. The third stage is the banana coating application using chitin solution to determine the shelf life of bananas with variations in levels of 2, 2.5, 3 and 3,5 % w/v by immersion method for one hour. It was found that carapace crab, a part that was underutilized from crab, gave rise to chitin deacetylation with a deacetylation rate of 62.11%; pH 8.9 and water content of 7.677%. Chitosan-based coatings are applied to fresh bananas and are found to increase fruit firmness, and inhibit browning. The results show that chitosan-coated bananas have a longer storage time. The application of chitin deacetylated (chitosan) as fruit banana coater found that higher coater levels extend the shelf life of bananas with the best coater content is 3% b/v. It results in a shelf life of bananas for up to 12 days, this is longer than bananas without chitosan layer which only has a shelf life of four days. Increased coating rates have a positive effect on the shelf life of bananas. This study shows that waste from carapace crabs can be used to form active layers that can preserve fruit.

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Section: Isolation Of Chitinmentioning

confidence: 99%

Potential of Chitosan From Local Crab (Portunus Pelagicus) to Enhance Storability of Musa Paradisiaca L.

Buanasari¹,

Sugiyo²,

Fitriani³

et al. 2019

JBAT

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“…Although the thermal profiles of the extracted samples are all very similarly shaped, the slopes of the curves, the exact points of degradation and heat flow are not consistent between the preliminary and optimised extracts. The slope of the curve and the exact point of degradation depend on the variable physical properties of the polymer structure [28], [29]. The raw samples are shown to have similar DSC profile shapes to the extracted samples but again degradation events and heat flow vary significantly between similar %DA samples.…”

Section: E Dscmentioning

confidence: 89%

Improving Extraction Processes Of Crustacean Chitin Using Solid State Analytical Techniques

F.Ó¹

2019

IJAC

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Solid state analytical techniques are becoming more widely used for the analysis of a range of organic products which demonstrate very poor solubility in both common organic and polar solvents and as such cannot be accurately characterised using solution based techniques. Primarily used as a secondary technique for qualitative analysis of insoluble intermediates and products in organic synthesis, 13 C CP-MAS NMR can be utilised in tandem with a targeted extraction and clean up procedure for accurate quantitative analysis of insoluble bio-molecules of interest. Here solid state 13 C CP-MAS NMR is utilised as the primary analytical technique in the characterisation of crustacean sourced chitin whereby Cancer pagurus crab shell chitin and Pandalus borealis shrimp shell chitin are shown to have a degree of acetylation greater than 90%. FTIR spectroscopy, Raman spectroscopy and DSC provide secondary structural, molecular and thermal analysis of the raw materials and extracted chitin.

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“…13 Chitosan can be easily generated from prawn and crab shells. 14,15 In a previous study on chitosan dissolved in citric acid, it was found that 0.1-0.6% chitosan citrate (CC) solution showed antibacterial activity, significantly better cleansing of the smear layer, and a lack of dentin erosion compared with 10% citric acid. A previous study using electronic micrographs showed that chitosan weakens and even damages Gram-positive bacterial cell walls and disrupts the cytoplasm membrane in Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

Comparing the effectivities of chitosan citrate and chitosan acetate in eradicating Enterococcus faecalis biofilm

et al. 2018

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Background: Adequate biomechanical preparations, antibacterial irrigants, and intracanal medications to promote the elimination of bacteria and their products are required to succeed root canal treatment. Enterococcus faecalis with its biofilm is known as an important etiological agent in endodontic treatment failures. Chitosan, as a natural product, has an antibacterial activity and is considered less toxic to the periapical tissue than other irrigants. However, the use of this natural product needs to be examined to determine its effectiveness as a root canal irrigant in endodontic treatment; this can be done by comparing it with the most common endodontic irrigant (NaOCl 5.25%) as a positive control. Objective: The objective of the study was to compare the effectiveness between 1-3% chitosan acetate (CA) and 1-3% chitosan citrate (CC) against E. faecalis biofilm formation after treatment for 15, 30, and 60 minutes. Methods: The study was conducted using 12 groups, including 1-3% CA, 1-3% CC, and control groups. E. faecalis biofilms in 96-well plates were exposed to each sample for 15, 30, or 60 minutes. Subsequently, the biofilms were stained with crystal violet solution, and the optical density value was measured using a microtiter plate reader at a wavelength of 600 nm. Results: CA and CC were effective in eradicating E. faecalis biofilm. However, the levels of effectiveness of CC and CA depended on the concentration and application time. Three-way analysis of variance (ANOVA) showed a significant difference between the irrigants (p <0.05) and three application times (p <0.05). The CA was effective in eradicating biofilm after 15 minutes of application, whereas the CC was more effective after 30 and 60 minutes of application. Conclusion: CC and CA are both effective in eradicating E. faecalis biofilm.

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Physicochemical properties and characterization of chitosan synthesized from fish scales, crab and shrimp shells

Cited by 261 publications

References 36 publications

Potential of Chitosan From Local Crab (Portunus Pelagicus) to Enhance Storability of Musa Paradisiaca L.

Potential of Chitosan From Local Crab (Portunus Pelagicus) to Enhance Storability of Musa Paradisiaca L.

Improving Extraction Processes Of Crustacean Chitin Using Solid State Analytical Techniques

Comparing the effectivities of chitosan citrate and chitosan acetate in eradicating Enterococcus faecalis biofilm

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