“…The degree of deacetylation (DDA) of chitosan ranges from 50 to 100% depending on the source (Trung & Bao, 2015), preparation method (demineralization, deproteinization, and deacetylation conditions) (Cho, No, & Meyers, 1998;Hossain & Iqbal, 2014;Lertsutthiwong, How, Chandrkrachang, & Stevens, 2002;Sagheer et al, 2009), and the reversal of the process steps (Fernandez-Kim, 2004). The DDA of chitosan influences both its physicochemical properties (Cho et al, 1998;Hossain & Iqbal, 2014;Tolaimate et al, 2000) and its biological properties (Matsugo et al, 1998;Raafat & Sahl, 2009;Rajalakshmi, Krithiga, & Jayachitra, 2013;Trung & Bao, 2015;Wan, Xu, Sun, & Li, 2013). Naznin (2005) stated that the optimum conditions for chitosan production from shrimp shell in Bangladesh were observed at using 30% HCI for demineralization, 1.5 N NaOH for deproteinization, 50% NaOH for deacetylation and resulted in chitosan yield of 8.7%.…”