Physicochemical properties and antioxidant activities of polysaccharide from floral mushroom cultivated in Huangshan Mountain

Wang, Junhui; Xu, Jian-Zhong; Zhang, Jingcheng; Liu, Yong; Sun, Hanju; Zha, Xue‐Qiang

doi:10.1016/j.carbpol.2015.05.052

Cited by 84 publications

(34 citation statements)

References 40 publications

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“…esculenta (100 g) was pulverized into powder, then defatted and decolorized in a Soxhlet extractor with acetone and ethyl acetate, respectively, for 48h each [26]. The defatted powder was then extracted twice with hot distilled water (1:30, w/v ) at 100°C for 2 h. The extracted solution was filtered through four pieces of gauzes to obtain the supernatants [8]. After filtration, the supernatants were combined and concentrated.…”

Section: Methodsmentioning

confidence: 99%

“…The homogeneity and molecular weight of UP2 were measured using high-performance gel permeation chromatography (HPGPC) [8] on a Waters E2695 high performance liquid chromatography (HPLC) system apparatus equipped with a Ultrahydrogel TM 2000 column (7.8 mm × 300 mm), a Ultrahydrogel TM 500 column (7.8 mm × 300 mm), and a Waters 2424 evaporative light scattering detector (Waters Corporation, USA). The sample was dissolved in ultrapure water at a concentration of 1.0 mg/mL and passed through a 0.22 μm filtration membrane.…”

Section: Methodsmentioning

confidence: 99%

“…UP2 (5.0 mg) was hydrolyzed with 2M TFA (4.0 mL) in a sealed tube at 120°C for 4 h. After removing the residual TFA with a rotary evaporator, the hydrolysate was dissolved in ultrapure water (3.0 mL) and reduced with Sodium borohydride (NaBH 4 ) (30 mg) at room temperature for 3 h. The resultant solution was neutralized with 25% acetic acid, and then evaporated to dryness in a rotary evaporator. Then, 3.0 mL of each acetic anhydride and pyridine were added, and the reaction was sealed and reacted at 100°C for 1 h. The resulting monosaccharides were converted into alditol acetate derivatives [8, 32] and analyzed by GC.…”

Section: Methodsmentioning

confidence: 99%

“…Complete methylation was confirmed by the disappearance of the OH band (3200–3700cm -1 ) from the IR spectrum. The methylated polysaccharide was then hydrolyzed, reduced, acetylated, and converted into methylated alditol acetates [8, 32–34]. The resulting alditol acetates were analyzed by gas chromatography-mass spectrometry (GC-MS).…”

Section: Methodsmentioning

confidence: 99%

“…They are essential biological macromolecules, and are directly involved in life processes, exhibiting a variety of important biological functions [2, 7]. Furthemore, polysaccharides exhibit immune-enhancement, anti-tumor, antioxidant, and other biological activities [8–9]. Consequently, much research attention has been focused on the extraction and purification, structural elucidation, biological and pharmacological effects, and structure-activity relationships of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

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Structure Elucidation of a Polysaccharide from Umbilicaria esculenta and Its Immunostimulatory Activity

Zhang

et al. 2016

PLoS ONE

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Umbilicaria esculenta has been used as a tonic food in China for several centuries owing to its pleasant flavor and health benefits. In this study, a water soluble polysaccharide, which we designated as UP2, with an average molecular weight of 3.33 × 105 Da, was isolated from U. esculenta cultivated in the Huangshan Mountain, by consecutive hot water extraction and anion-exchange chromatography. Gas chromatography analysis indicated that UP2 contained three kinds of monosaccharides, including mannose, glucose, and galactose at a molar ratio of 1.7:1.0:1.2. Linkage analysis of UP2 revealed the presence of (1 → 6)-linked glucosyl, (1 → 3,6)-linked glucosyl, t-linked galactosyl, (1 → 6)-linked galactosyl and (1 → 6)-linked mannosyl at a molar ratio of 0.7:4.6:4.1:2.2:9.1. Structural analysis determined that UP2 possessed a backbone consisting of (1 → 6)-linked β-D-glucopyranosyl and (1 → 6)-linked α-D-mannopyranosyl residues, which substituted at the O-3 position of (1 → 6)-linked β-D-glucopyranosyl residues by branches of (1 → 6)-linked α-D-galactopyranosyl and 1-linked β-D-galactopyranosyl residues. Immunostimulatory activity analysis showed that UP2 could stimulate the proliferation of RAW264.7 cells in a dose-dependent manner, and all the samples (20–500 μg/mL) were found to enhance nitric oxide production. The highest phagocytic activity of UP2 was observed at 200 μg/mL. Thus, UP2 may be a potential source of biological and pharmacological agents.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structure Elucidation of a Polysaccharide from Umbilicaria esculenta and Its Immunostimulatory Activity

Zhang

et al. 2016

PLoS ONE

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Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota‐Dependent Manner

Zou

Wang

Liu

et al. 2022

Molecular Nutrition Food Res

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Scope Ulcerative colitis (UC) is a common chronic recurrent inflammatory bowel disease. This study attempts to reveal the improvement mechanism of floral mushroom polysaccharide (FMPS) on UC from the perspective of coordinated interaction between intestinal microbes and intestinal helper T cell 17 (Th17)/regulatory T cell (Treg) balance. Methods and results Dextran sulfate sodium (DSS)‐induced colitis mice model is used for the experiment. The results suggest that FMPS up‐regulated the expression of occludin, ZO‐1, and MUC2, and down‐regulated the secretion of TNF‐α, IL‐1β, and IL‐6 in colitis mice. Importantly, FMPS restores intestinal Th17/Treg balance. Meanwhile, FMPS can regulate intestinal microorganisms and improve the level of short‐chain fatty acids (SCFAs) in colitis mice. Intestinal microbial depletion and fecal microbiota transplantation (FMT) experiments reveal that FMPS ameliorated UC is mediated by intestinal microbiome. Flow cytometry further proves that FMPS restores intestinal Th17/Treg balance in a microbial‐dependent manner. Conclusion These results indicate that FMPS has the potential to improve UC, and its mechanism depends on the restoration of Th17/Treg balance mediated by intestinal microorganisms. Therefore, it is suggested that FMPS dietary supplement can be potentially used to intervene UC.

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Edible Mushrooms as Neuro-nutraceuticals: Basis of Therapeutics

Remya

Chandra

Mohanakumar

2019

Medicinal Mushrooms

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Physicochemical properties and antioxidant activities of polysaccharide from floral mushroom cultivated in Huangshan Mountain

Cited by 84 publications

References 40 publications

Structure Elucidation of a Polysaccharide from Umbilicaria esculenta and Its Immunostimulatory Activity

Structure Elucidation of a Polysaccharide from Umbilicaria esculenta and Its Immunostimulatory Activity

Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium‐Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota‐Dependent Manner

Edible Mushrooms as Neuro-nutraceuticals: Basis of Therapeutics

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