Physicochemical, cytotoxicity and in vivo biocompatibility of a high-plasticity calcium-silicate based material

Abstract

The purpose of this work was to evaluate the physicochemical properties, the cytotoxicity and in vivo biocompatibility of MTA Repair HP (MTA HP) and White MTA (WMTA). The setting time, flow, radiopacity and water solubility were assessed. To the cytotoxicity assay, primary human osteoblast cells were exposed to several dilutions of both materials eluates. MTT assay, apoptosis assay and cell adhesion assay were performed. The in vivo biocompatibility was evaluated through histological analysis using different staining techniques. No differences were observed between MTA HP and WMTA for setting time, radiopacity, solubility and water absorption (P > 0.05). However, MTA HP showed a significantly higher flow when compared to WMTA (P < 0.05). Cell viability results revealed that the extracts of WMTA and MTA HP promoted the viability of osteoblasts. After incubation of cells with the endodontic cement extracts, the percentage of apoptotic or necrotic cells was very low (<3%). Furthermore, SEM results showed a high degree of cell proliferation and adhesion on both groups. MTA HP showed similar in vivo biocompatibility to the WMTA and the control group in all time-points. The MTA HP presented adequate physicochemical and biological properties with improved flow ability when compared to WMTA. Such improved flow ability may be a result of the addition of a plasticizing agent and should be related to an improvement in the handling of MTA HP.

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

“…The introduction of mineral trioxide aggregate (MTA) allowed further enhanced application and significant improvement of properties with the purpose of achieving the maximum benefits. Numerous reports and studies confirm that with respect to various clinical procedures, MTA is considered as the golden standard material [ 1 , 2 ]. With respect to its biocompatibility, bioactivity and hydrophilicity, as well as radiodensity, sealing ability and low solubility, MTA shows several desirable properties.…”

Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer

“…In endodontic research, it is very important to know the toxicity or biocompatibility of new materials prior to their clinical application, as the compounds may potentially damage surrounding tissues [1,2]. However, new bioactive materials have been developed for use in dentistry, with a variety of clinical applications, ranging from vital pulp therapy to root canal filling, root-end filling, apexification and perforation repair [3,4,5].…”

The Effectiveness of Laser-Activated Irrigation on the Apical Microleakage Qualities of MTA Repair HP and NeoMTA Plus in Simulated Immature Teeth: A Comparative Study

“…The differences observed between two tested BEC S could be attributed to the physicochemical formulation of MTA Repair HP. The addition of a polymer plasticizer to the liquid structure of MTA Repair HP provides a better putty and homogeneous compositions that increase fluidity during manipulation procedures.Moreover, the low initial setting time of MTA Repair HP may lead to variations by increasing the effective biological responses of hydroxyapatite surfaces and creating hydrated gel forms to fill spaces between calcium silicate particles [ 33 , 34 ]. Hence, the short-term findings of the present study between MTA Repair HP and NeoMTA Plus are supported.…”