Physicochemical Characterization, Stability, and In Vitro Evaluation of Curcumin-Loaded Solid Lipid Nanoparticles Prepared Using Biocompatible Synthetic Lipids

Abstract

Solid lipid nanoparticles (SLNs) are promising drug delivery vehicles for the delivery of various drugs, especially poorly water-soluble drugs. However, the aqueous stability, drug release, and biocompatibility of SLNs are some of the issues that need attention. In this work, curcumin-loaded SLNs were prepared, and morphology, particle size, and entrapment efficiency were studied. For this, two amino acid-derived lipids were developed. The effect of the polarity of the lipid head on the aqueous stability of the SLN dispersion was investigated. Based on the stability, particle size, and polydispersity, an optimum formulation was obtained. The curcumin entrapment efficiency of the SLNs was found to be greater than those reported in the literature. The entrapped curcumin, as well as curcumin-loaded SLN suspensions, exhibited improved storage stability. The in vitro release kinetics indicated an enhanced rate of drug release in the case of curcumin-loaded SLNs consisting of the lipid containing −OH groups at the lipid head. The pure lipid and the blank SLN were found to have no significant cytotoxicity, but curcumin and curcumin-loaded SLNs induced cell death in a concentration-dependent manner in both human prostatic adenocarcinoma PC3 cell line and human breast carcinoma MCF7 cell line. This study has proposed a potential semisynthetic lipid for the stable SLN suspension for the delivery of curcumin.

Delivery of Chlorambucil to the Brain Using Surface Modified Solid Lipid Nanoparticles

“…The blank SLN was visually found to be stable for at least 45 days at room temperature. This is partly due to the presence of the hydrophilic −OH group at the lipid head . Incorporation of the C 14 -GAHBA lipid in the formulation of SLNs provides an ideal hydrophobic core that effectively encapsulates hydrophobic drugs.…”

“…SLNs were prepared following a hot homogenization and ultrasonication method. , In a hot water bath at 85–90 °C, solid lipid (100 mg) and CLB ((0–10 mg) were gently melted and mixed. Meanwhile, Milli-Q water was used to dissolve Tween 80 (T80), which was then heated to the same temperature range while constantly stirred.…”

“…A known volume of the supernatant liquid was then dissolved in methanol, and absorbance was measured on a UV–vis spectrophotometer (Shimadzu UV-2450, Japan) at a wavelength of 258 nm. The EE% was calculated using the eq : , EE .25em ( % ) = ( w l / w i ) 100 where w i represents the drug’s initial weight, and w l represents how much of the drug was trapped inside the nanoparticles. Similarly, LE% was calculated using eq : ( LE % ) = ( w 1 / w l ) 100 where w l is the weight of the lipid employed.…”

“…Before usage, methanol was newly distilled and dried. The synthesis and chemical identification of C 14 -GAHBA lipid has already been described in our earlier publication . Milli-Q water (resistivity >18.2 MΩ cm) was employed for the preparation of aqueous solutions.…”

“…GABA receptors are not only overexpressed in various cancers related to CNS like glioma, neuroblastoma, and medulloblastoma but also in various other cancers like breast, lung, liver, etc. , It has been documented in the literature how GABA functions chemically, physiologically, and pharmacologically. − The interaction of the SLN with chiral compounds can also be improved by the chiral headgroup of C 14 -GAHBA. In our earlier publication, in vitro studies have shown that C 14 -GAHBA exhibits no significant cytotoxicity effect. The storage stability of the SLN suspension has also been seen to be improved by the presence of free −OH and −COOH groups in the lipid structure.…”