Physicochemical characterization of Gracilaria chouae sulfated polysaccharides and their antioxidant potential

Khan, Bilal Muhammad; Qiu, Hua-Mai; Wang, Xuefei; Liu, Zhuoyan; Zhang, Jiayan; Guo, Yan-Jun; Chen, Weizhou; Liu, Yang; Cheong, Kit-Leong

doi:10.1016/j.ijbiomac.2019.05.055

Cited by 44 publications

(35 citation statements)

References 58 publications

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“…The SEC chromatograms of JSP showed various peaks (DRI and LS), suggesting the presence of various molecular masses and a significant degree of dispersion (data not shown). The PDI value showed JSP heterogeneity and could be due to the presence of floridean starch (α-Glc) contamination and other larger molecular weight xylogalactans [49,50]. This last statement is consistent with the literature concerning sulfated galactans from Botryocladia occidentalis [51] and Gracilaria cornea [52] red seaweeds.…”

Section: Macromolecular Characteristics Of Jspsupporting

confidence: 89%

“…Polymer intrinsic viscosity ([η] mL/g) indicates the capacity of polymers to enhance the viscosity of fluids, dependent on their physicochemical properties, such as structural conformation, type and degree of branching units, M w , and solvent property [33,49]. The [η] of 76 mL/g and the hydrodynamic radius R h of 16.8 nm obtained for JSP suggest a flexible random coil conformation with a value of Mark-Houwink-Sakurada exponent α ranging between 0.5 and 0.8.…”

Section: Macromolecular Characteristics Of Jspmentioning

confidence: 99%

“…The [η] of 76 mL/g and the hydrodynamic radius R h of 16.8 nm obtained for JSP suggest a flexible random coil conformation with a value of Mark-Houwink-Sakurada exponent α ranging between 0.5 and 0.8. [33,49,56]. Finally, the theoretical critical concentration C* of JSP, which can be obtained by ks/[η] (with ks = 0,5 to 1 for coil polysaccharides in water) could range from 6.5 to 13.1 g/L.…”

Section: Macromolecular Characteristics Of Jspmentioning

confidence: 99%

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Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

et al. 2020

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A novel sulfated xylogalactan-rich fraction (JSP for J. adhaerens Sulfated Polysaccharide) was extracted from the red Tunisian seaweed Jania adhaerens. JSP was purified using an alcoholic precipitation process and characterized by Attenuated Total Reflectance-Fourier-transform infrared spectroscopy (ATR-FTIR), high-pressure size exclusion chromatography (HPSEC) with a multi-angle laser light scattering (MALLS), gas chromatography coupled to mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR, 1D and 2D). JSP was then evaluated regarding its physicochemical and rheological properties. Results showed that JSP was mainly composed of an agar-like xylogalactan sharing the general characteristics of corallinans. The structure of JSP was mainly composed of agaran disaccharidic repeating units (→3)-β-d-Galp-(1,4)-α-l-Galp-(1→) n and (→3)-β-d-Galp-(1,4)-3,6-α-l-AnGalp-(1→) n , mainly substituted on O-6 of (1,3)-β-d-Galp residues by β-xylosyl side chains, and less with sulfate or methoxy groups. (1,4)-α-l-Galp residues were also substituted by methoxy and/or sulfate groups in the O-2 and O-3 positions. Mass-average and number-average molecular masses (M w ) and (M n ), intrinsic viscosity ([η]) and hydrodynamic radius (R h ) for JSP were, respectively, 8.0 × 10 5 g/mol, 1.0 × 10 5 g/mol, 76 mL/g and 16.8 nm, showing a flexible random coil conformation in solution. The critical overlap concentration C* of JSP was evaluated at 7.5 g/L using the Williamson model. In the semi-diluted regime, JSP solutions displayed a shear-thinning behavior with a great viscoelasticity character influenced by temperature and monovalent salts. The flow characteristics of JSP were described by the Ostwald model. always belongs to the d-series, while the former (α-Gal) can occur as d-(carrageenans) or l-(agarans) series [4][5][6][7]. The main backbone can be substituted by pyruvic acid ketals, sulfate, O-glycosyl and/or methyl groups and also by side chains, giving rise to great structural changes along the galactan chain. These structural changes affect the physicochemical and rheological behaviors of these polymers. So far, it has been reported that sulfated galactans exhibit numerous biological properties, including antioxidant, anticoagulant, antinociceptive, immunomodulatory, anticancer, gastroprotective, anti-inflammatory, antithrombotic and antiproliferative [1,[6][7][8][9][10][11][12][13][14]. Marine seaweed (Phaeophyceae and Rhodophyceae) cell wall polysaccharides also hold great promise as a raw material for feed, food, and biofuel production. Through metabolic engineering, seaweeds (micro-and macroalgae) are attracting growing attention as an alternative source of biofuel by converting the seaweed biomass and, in particular, the cell wall, which is rich in carbohydrates, into liquid biofuels [15].Galactans are currently used as thickening, gelling, and stabilizing agents in food, cosmetic, and pharmaceutical fields [1,10-16]. However, sulfated galactans produced by Corallinales are less studied, probably because ...

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Section: Macromolecular Characteristics Of Jspsupporting

confidence: 89%

Section: Macromolecular Characteristics Of Jspmentioning

confidence: 99%

Section: Macromolecular Characteristics Of Jspmentioning

confidence: 99%

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Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

et al. 2020

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“…The activity of BSPs samples in scavenging ABTS radicals was evaluated as described earlier, but with some minor modifications [17]. To generate ABTS radicals, a 5 mL ABTS solution (7 mmol/L) was mixed with 88 µL K 2 S 2 O 8 (149 mmol/L), and the reaction mixture was incubated for 16 h (dark).…”

Section: Abts Radical Scavenging Activitymentioning

confidence: 99%

“…Then, 1 mL of this solution was added to 10 µL of BSPs samples at various concentrations, and the absorbance was measured at 734 nm. ABTS radical scavenging activity (%) was calculated as previously described [17]. The 50% inhibitory concentration (IC 50 ) was defined as the concentration of BSPs samples that inhibited DPPH and ABTS radical formation by 50%.…”

Section: Abts Radical Scavenging Activitymentioning

confidence: 99%

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

et al. 2021

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Brown seaweed polysaccharides (BSPs) are one of the primary active components from brown seaweed that has a range of pharmaceutical and biomedical applications. However, the quality control of BSPs is a challenge due to their complicated structure and macromolecule. In this study, saccharide mapping based on high-performance liquid chromatography (HPLC), multi-angle laser light scattering, viscometer, and refractive index detector (HPSEC-MALLS-Vis-RID), and Fourier transform infrared (FT-IR) were used to discriminate the polysaccharides from nine different species of brown algae (BA1-9). The results showed that BSPs were composed of β-D-glucans and β-1,3−1,4-glucan linkages. The molecular weight, radius of gyration, and intrinsic viscosity of BSPs were ranging from 1.718 × 105 Da to 6.630 × 105 Da, 30.2 nm to 51.5 nm, and 360.99 mL/g to 865.52 mL/g, respectively. Moreover, α values of BSPs were in the range of 0.635 to 0.971, which indicated a rigid rod chain conformation. The antioxidant activities of BSPs exhibited substantial radical scavenging activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radicals, which indicated that the use of BSPs might be a potential approach for antioxidant supplements. Thus, this study gives insights about the structure-function relationship of BSPs, which will be beneficial to improve the quality of polysaccharides derived from marine algae.

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Algal Polysaccharides and Their Biological Properties

Cheong

Jesumani

Khan

et al. 2021

Recent Advances in Micro and Macroalgal Processing

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Physicochemical characterization of Gracilaria chouae sulfated polysaccharides and their antioxidant potential

Cited by 44 publications

References 58 publications

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

Structural Features and Rheological Properties of a Sulfated Xylogalactan-Rich Fraction Isolated from Tunisian Red Seaweed Jania adhaerens

Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

Algal Polysaccharides and Their Biological Properties

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