Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

Abstract

Brown seaweed polysaccharides (BSPs) are one of the primary active components from brown seaweed that has a range of pharmaceutical and biomedical applications. However, the quality control of BSPs is a challenge due to their complicated structure and macromolecule. In this study, saccharide mapping based on high-performance liquid chromatography (HPLC), multi-angle laser light scattering, viscometer, and refractive index detector (HPSEC-MALLS-Vis-RID), and Fourier transform infrared (FT-IR) were used to discriminate the polysaccharides from nine different species of brown algae (BA1-9). The results showed that BSPs were composed of β-D-glucans and β-1,3−1,4-glucan linkages. The molecular weight, radius of gyration, and intrinsic viscosity of BSPs were ranging from 1.718 × 105 Da to 6.630 × 105 Da, 30.2 nm to 51.5 nm, and 360.99 mL/g to 865.52 mL/g, respectively. Moreover, α values of BSPs were in the range of 0.635 to 0.971, which indicated a rigid rod chain conformation. The antioxidant activities of BSPs exhibited substantial radical scavenging activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radicals, which indicated that the use of BSPs might be a potential approach for antioxidant supplements. Thus, this study gives insights about the structure-function relationship of BSPs, which will be beneficial to improve the quality of polysaccharides derived from marine algae.

Synthesis and Evaluation of Biological Activities of Bis(spiropyrazolone)cyclopropanes: A Potential Application against Leishmaniasis

“…Thus, our laboratory has worked on the synthesis of 4,4′-(arylmethylene)bis(1-phenyl-3-methyl-1 H -pyrazole-5-ols) and assayed them against in vitro models previously implemented in our laboratory. We found that these compounds have good antioxidant activity in vitro, as well as cytotoxicity against the RKO cell line [ 12 ]. We found good leishmanicidal activity against promastigotes of Leishmania mexicana and the evaluation of the anti-trypanosomatid activity of these compounds is in progress and will be reported elsewhere.…”

Tuning Anti-Biofilm Activity of Manganese(II) Complexes: Linking Biological Effectiveness of Heteroaromatic Complexes of Alcohol, Aldehyde, Ketone, and Carboxylic Acid with Structural Effects and Redox Activity

“…A large number of heteroaromatic compounds containing pyridine or imidazole core are associated with diverse pharmacological properties such as antimicrobial, anticonvulsant, antiviral, antifungal, etc. [ 25 , 26 , 27 , 28 , 60 ].…”

“…According to the reports, functional groups containing oxygen and sulfur donors have a potential role in fighting cancer cells and bacteria [ 22 , 23 ]. Additionally, imidazole and pyridine are well-known pharmacists whose derivatives are currently used against various diseases [ 24 , 25 , 26 , 27 , 28 ]. In our research, we employed the N,O-donor ligands, in which imidazole and pyridine were substituted by -OH, -CHO, and -COOH functional groups.…”

A Compendium of the Most Promising Synthesized Organic Compounds against Several Fusarium oxysporum Species: Synthesis, Antifungal Activity, and Perspectives

“…Azole compounds of the type 1,2,3-triazoles attract more attention as they have excellent biological and physical properties [ 116 , 117 ]. These heterocycles can be obtained by the copper (I) catalyzed 1,3-dipolar cycloaddition reaction of azide and alkyne, as shown in previous reports.…”