Physicochemical Characterization of an Exopolysaccharide Produced by a Newly Isolated Weissella cibaria

Vasanthakumari, D.S.; Harikumar, S; Beena, Divya Jayakumar; Pandey, Ashok; Nampoothiri, K. Madhavan

doi:10.1007/s12010-015-1586-2

Cited by 32 publications

(11 citation statements)

References 30 publications

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“…citreum B-2Homemade fermentation product of pineapple75 g/l sucroseGlucan3.77 × 10 6 28.3Lyophilized dry weight[51] W. confusa OF126Ogi24.0 g/l sucrose, pH (7.00)Glucan1.1 × 10 6 3.10Lyophilized dry weight[50] Leu. Mesenteroides 109Plant20 g/l of fructose, 50 g/l of sucroseGlucan1.4 × 10 6 19.0Polymer dry mass 42 °C 48 h[52] W. cibaria Fermented sauropus androgynusSucroseGlucan–6.4Lyophilized dry weight[45] W. sp. PSMS4-4Plasom5% White-sugar 30 °CGlucan–8.7Phenol sulfuric acid method [89]Glucose[91]…”

Section: Resultsmentioning

confidence: 99%

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Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces

et al. 2019

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Background Lactic acid bacteria (LAB) are known to have a significant ability to colonize the human intestinal tract and adhere to the surface of intestinal epithelial cells. Among the various lactic acid bacteria, exopolysaccharide (EPS) producing strains are known to provide a variety of health benefits for their hosts (e.g. anti-inflammatory, antioxidant, antitumor and stress tolerant effects). Recently, EPSs and EPS-producing lactic acid cultures have gained interest within the food industry and are playing important roles as biothickeners and texturizing agents due to their hydrocolloidal nature. In this study, 156 lactic acid bacterial strains isolated from fecal samples of healthy young children were screened and evaluated for active EPS-production capability. Results Among the various human origin lactic acid flora isolated, Weissella confusa VP30 showed the highest EPS productivity and its EPS producing properties were characterized under various cultural conditions in this research. To document the safety of W. confusa VP30, antibiotic resistance, hemolytic, and ammonia production properties were evaluated in addition. No significant negative results were observed. The maximum EPS production by W. confusa VP30 was 59.99 ± 0.91 g/l after 48 h of cultivation in media containing 10% sucrose, far exceeding EPS production by other bacterial strains reported elsewhere. Based on gel permeation chromatography (GPC), the molecular weight of EPS produced by W. confusa VP30 was 3.8 × 10 6 Da. Structural analysis of the released EPS fraction by 13 C and 1 H nuclear magnetic resonance (NMR) spectroscopy revealed that W. confusa VP30 can produce dextran with glucose units linked with 96.5% α (1 → 6) glycosidic bonds and 3.5% α (1 → 3) branches. Conclusion The high EPS production capability and safety of W. confusa VP30 justify food industry consideration of this cell strain for further evaluation and potential industrial use.

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Section: Resultsmentioning

confidence: 99%

“…Waters Ultrahydrogel 120, Waters Ultrahydrogel 500 and Waters Ultrahydrogel 1000), where a typical sharp symmetrical peak was observed (Fig. 2) [44, 45]. W. confusa VP30 produced 3.8 × 10 6 Da EPS which is significantly larger than those from other Weissella spp.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces

et al. 2019

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“…Moreover, all Lactobacillus strains considered in this study were able to produce large quantities of EPS, compared to other typical lactobacilli isolated from diverse sources [52,53]. The EPS structure can act as a capsule bound to the cell surface, allowing protection against toxic agents and stress conditions found during desiccation, or osmotic stress and acidity conditions [54,55].…”

Section: Discussionmentioning

confidence: 97%

Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Barache

Belguesmia

Ladjouzi

et al. 2020

Foods

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Nine strains of Lactiplantibacillus plantarum and one strain of Lacticaseibacillus paracasei that were recently isolated from prickly pears, fresh figs and blackberries, which are traditionally and largely consumed fruits in Kabylia (north of Algeria), were studied here for their antagonism and antioxidant properties as well as for production of exopolysaccharides. With respect to their inhibitory properties, these strains were tested against three food representative pathogens including Escherichia coli ATCC 8739, Staphylococcus aureus 2S6 and Listeria monocytogenes 162. The antagonism of these pathogens was attributable to lactic acid production, present in the cell free supernatant, at concentrations ranging from 9 to 16.74 g/L. The anti-adhesive properties observed on polystyrene or eukaryotic Caco-2 cells were exerted in a strain dependent-manner. Indeed, the scores obtained ranged from 27% to 75% for S. aureus 2S6, 54% to 95% for L. monocytogenes 162, and 50% to 97% for E. coli ATCC 8739. The co-aggregation of these Lactobacillus strains with the aforementioned target bacteria appeared to be exerted in a strain-dependent manner, with noticeably the upmost rate for Lb. paracasei FB1 on S. aureus 2S6. Interestingly, these novel Lactobacillus strains were able to produce a large amount (315.55 to 483.22 mg/L) of exopolysaccharides, and showed a significant scavenging activity on the 2,2-di-phényl-2-picrylhydrazyle (DPPH) synthetic free radical with rates of 51% to 56%. Of note, the highest antioxidant activity was observed for Lb. paracasei FB1 using the culture supernatants, intact cells or the intracellular extract. The statistical analysis of these data using the principal component analysis (ACP) enabled us to establish three distinct clusters with potential applications as bioprotective and/or probiotic agents, following further evaluation.

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“…Regarding the EPS, the observed broad peak at 3225 cm -1 is related to the stretching vibrations of O-H [50] and the sharp peak observed at 2936 cm -1 is due to the stretching vibrations of C-H indicating the presence of the CH3 functional group [51]. The peaks at 1566 cm -1 and 1418 cm -1 can be attributed to C=O stretching and C-H bending vibrations of carboxylic and CH3 and/or CH2 functional groups, respectively [50].…”

Section: The Eps Extraction Purification and Characterizationmentioning

confidence: 98%

Anionic exopolysaccharide from Cryptococcus laurentii 70766 as an alternative for alginate for biomedical hydrogels

Hamidi

Jafari

Simińska-Stanny

et al. 2022

International Journal of Biological Macromolecules

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Physicochemical Characterization of an Exopolysaccharide Produced by a Newly Isolated Weissella cibaria

Cited by 32 publications

References 30 publications

Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces

Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces

Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Anionic exopolysaccharide from Cryptococcus laurentii 70766 as an alternative for alginate for biomedical hydrogels

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