Clusters of Lactobacillus Strains from Vegetal Origins Are Associated with Beneficial Functions: Experimental Data and Statistical Interpretations

Abstract

Nine strains of Lactiplantibacillus plantarum and one strain of Lacticaseibacillus paracasei that were recently isolated from prickly pears, fresh figs and blackberries, which are traditionally and largely consumed fruits in Kabylia (north of Algeria), were studied here for their antagonism and antioxidant properties as well as for production of exopolysaccharides. With respect to their inhibitory properties, these strains were tested against three food representative pathogens including Escherichia coli ATCC 8739, Staphylococcus aureus 2S6 and Listeria monocytogenes 162. The antagonism of these pathogens was attributable to lactic acid production, present in the cell free supernatant, at concentrations ranging from 9 to 16.74 g/L. The anti-adhesive properties observed on polystyrene or eukaryotic Caco-2 cells were exerted in a strain dependent-manner. Indeed, the scores obtained ranged from 27% to 75% for S. aureus 2S6, 54% to 95% for L. monocytogenes 162, and 50% to 97% for E. coli ATCC 8739. The co-aggregation of these Lactobacillus strains with the aforementioned target bacteria appeared to be exerted in a strain-dependent manner, with noticeably the upmost rate for Lb. paracasei FB1 on S. aureus 2S6. Interestingly, these novel Lactobacillus strains were able to produce a large amount (315.55 to 483.22 mg/L) of exopolysaccharides, and showed a significant scavenging activity on the 2,2-di-phényl-2-picrylhydrazyle (DPPH) synthetic free radical with rates of 51% to 56%. Of note, the highest antioxidant activity was observed for Lb. paracasei FB1 using the culture supernatants, intact cells or the intracellular extract. The statistical analysis of these data using the principal component analysis (ACP) enabled us to establish three distinct clusters with potential applications as bioprotective and/or probiotic agents, following further evaluation.

Nicotinamide Mononucleotide Combined With Lactobacillus fermentum TKSN041 Reduces the Photoaging Damage in Murine Skin by Activating AMPK Signaling Pathway

“…With a slight modification according to the literature method ( Xu et al, 2018 ; Barache et al, 2020 ), the bacteria were collected by centrifugation and washed three times with PBS (pH 7.4, 0.01M) after L. fermentum TKSN041 was cultured three times. Then the concentration of the bacterial suspension was adjusted to 1 × 10 9 CFU/ml, and it was treated in an ultrasonic disintegrator (KunShan Ultrasonic Instruments Co., Ltd., China) at 100 W for 30 min.…”

Biopreservation of Fresh Sardines (Sardina pilchardus) Using Lactiplantibacillus plantarum OV50 Isolated from Traditional Algerian Green Olives Preparations

“…The selection of LAB strains as natural biopreservatives is based on their inhibitory properties [29]. We isolated 17 novel antagonistic LAB strains from traditional Algerian olive preparations and related their inhibitory properties to the production of organic acids, especially lactic acid since the isolates were homofermentative.…”

Preventive and therapeutic effect of Lactobacillus paracasei ZFM54 on Helicobacter pylori-induced gastritis by ameliorating inflammation and restoring gastric microbiota in mice model

“…Co-aggregation is considered a key strategy for Lactobacillus strains to prevent pathogenic bacteria from adhering to biological surfaces ( 36 ). Barache et al analyzed that L. paracasei FB1 showed stronger co-aggregation with S. aureus (28.34% ± 2.56%) after 2 h of incubation, followed by E. coli (23.39 ± 2.12%) and L. monocytogenes (20.10 ± 2.04%) ( 37 ). L. paracasei ZFM 54 displayed a co-aggregation rate of over 30% and exhibited their capability to agglutinate H. pylori in vitro .…”