Physicochemical and rheological properties of crude polysaccharides extracted from <i>Tremella fuciformis</i> with different methods

Wang, Ling; Wu, Qin; Zhao, Jiayuan; Lan, Xuyue; Yao, Kai; Jia, Dongying

doi:10.1080/19476337.2021.1884607

Cited by 29 publications

(24 citation statements)

References 32 publications

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“…The molecular weight of TFPs was determined by a gel permeation chromatography instrument (PL-GPC50, Varian, Inc., Shropsire, UK) based on the method of Wang et al [16] . Chromatographic conditions were set as follows: a chromatographic column (7.5 × 300 mm, PLgelOlexis, Varian, Inc., Shropsire, UK), a protection column (7.5 × 50 mm, PLgelOlexis), a mobile phase of ultrapure water, a flow rate of 1.0 mL/min, and a column temperature of 40 °C.…”

Section: Methodsmentioning

confidence: 99%

Effects of combined ultrasonic and microwave vacuum drying on drying characteristics and physicochemical properties of Tremella fuciformis

Wang

Lei

et al. 2022

Ultrasonics Sonochemistry

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Section: Methodsmentioning

confidence: 99%

Effects of combined ultrasonic and microwave vacuum drying on drying characteristics and physicochemical properties of Tremella fuciformis

Wang

Lei

et al. 2022

Ultrasonics Sonochemistry

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“…However, only the TPS80 fraction showed an increased G' value after the crossover point. The results of TPS20, TPS40, and TPS60 were different from the results of Wang et al (L. Wang et al, 2021) may due to the concentration of ethanol while precipitation. Among four TPS solutions, TPS20 was the first to show an elastic characteristic.…”

Section: Dynamic Viscoelastic Propertiesmentioning

confidence: 57%

Physicochemical and rheological properties of Tremella fuciformis polysaccharide fractions by ethanol precipitation

Lan

Wang²,

Deng

et al. 2021

CyTA - Journal of Food

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Four Tremella fuciformis polysaccharides (TPS) fractions named as TPS20, TPS40, TPS60, and TPS80 were obtained by stepwise ethanol precipitation. Their physicochemical and rheological properties were investigated. Results showed TPS60 had the highest contents of polysaccharide (81.12%), protein (0.29%), uronic acid (14.36%), and sulfate (5.92%). Their molecular weights were in a descending order of TPS20, TPS40, TPS60, and TPS80. In a certain range of shear rate, they all presented pseudoplastic fluid and viscosity characteristic at low frequency. Increasing temperature or concentration of salts decreased the viscosity of their solutions. Sucrose concentration less than 6% led to an increasing viscosity of TPS20, TPS40, and TPS60. Both strong acidity and alkalinity could reduce the viscosity of all TPS solutions. TPS80 solution showed the best stability and its viscosity was minimally affected by pH, temperature, salts, and sucrose. These results can provide theoretical references for utilizations of TPS fractions in foods, pharmaceuticals, and cosmetics. Propiedades fisicoquímicas y reológicas de las fracciones de polisacáridos de Tremella fuciformis obtenidas por precipitación en etanol RESUMENPara el presente estudio se obtuvieron, mediante precipitación por etapas en etanol, cuatro fracciones de polisacáridos de Tremella fuciformis (TPS) denominadas TPS20, TPS40, TPS60 y TPS80. Se investigaron sus propiedades fisicoquímicas y reológicas y los resultados dieron cuenta de que la TPS60 tiene el mayor contenido de polisacáridos (81.12%), proteínas (0.29%), ácido urónico (14.36%) y sulfato (5.92%). Además, se comprobó que sus pesos moleculares estaban en un orden descendente de TPS20, TPS40, TPS60 y TPS80, constatándose que, en un determinado rango de velocidad de cizallamiento, todas las fracciones presentaban un fluido pseudoplástico y una viscosidad característica a baja frecuencia. Asimismo, tanto el aumento de la temperatura como de la concentración de sales disminuyó la viscosidad de sus soluciones, en tanto que la concentración de sacarosa inferior a 6% provocó un incremento de la viscosidad de TPS20, TPS40 y TPS60. Aunque la acidez y la alcalinidad fuertes pueden reducir la viscosidad de todas las soluciones de TPS, la solución TPS80 presentó la mejor estabilidad y su viscosidad se vio mínimamente afectada por el pH, la temperatura, las sales y la sacarosa. En conclusión, estos resultados pueden proporcionar referencias teóricas para la utilización de fracciones de TPS en alimentos, productos farmacéuticos y cosméticos.

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“…RPs-3 > RPs-1 > RPs-4 > RPs-5 > RPs-2, and the apparent viscosity of RPs-3 was significantly higher than the other groups at different shear rate (p < .05); it was speculated that this was due to the acid extraction environment further strengthening the tightness of RPs molecules, resulting in an increase in the apparent viscosity of the sample; however, the apparent viscosity of RPs-2 was obviously lower than the other groups (p < .05), which was speculated that the initial molecules of polysaccharide were rearranged under the polysaccharide concentration increased, the polysaccharide molecules began to overlap and connected to form a "junction zone," which restricted the movement and stretching of the polymer chain in the water system, leading to a significant increase in the apparent viscosity of samples (Wang, Wu, et al, 2021). Furthermore, the apparent viscosity of RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 was negatively correlated with the shear rate, indicating that the aqueous solution exhibited the shear thinning behavior (Hentati et al, 2020).…”

Section: Effects Of Extraction Methods On Apparent Viscosity Of Rpsmentioning

confidence: 86%

“…Anhydrous ethanol was added to the filtrate at a volume ratio of 3:1 to precipitate the polysaccharides at 4℃ for 24 hr. Later, the residues were collected, and the precipitate was dried with a vacuum freeze dryer (FD-1; BoYiKang Technology Company) to obtain the RPs-2 (Wang, Wu, et al, 2021).…”

Section: Alkaline Extractionmentioning

confidence: 99%

“…It possess a variety of significant biological activities, such as antioxidant (Yang et al, 2020), anti-inflammatory (Wu et al, 2021), anticancer (Samrot et al, 2019), antitumor (Chen et al, 2020), hypoglycemic (Hao et al, 2020), regulate intestinal flora (Tian et al, 2021), and osteoporosis prevention (Wang, Tu, et al, 2021). At present, common methods of extracting natural polysaccharides mainly include ultrasonic-assisted extraction (Mtetwa et al, 2020), alkaline extraction (Wang, Wu, et al, 2021), acid extraction (Gao et al, 2017), microwave-assisted extraction (Berkani et al, 2020), and three-phase separation (Yan et al, 2020), and so on. However, accumulating studies have reported that the physicochemical properties and biological activities of polysaccharides were regularly affected by the extraction methods (Chen et al, 2018;Gu et al, 2020;Park & Lee, 2021).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

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The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

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Physicochemical and rheological properties of crude polysaccharides extracted from Tremella fuciformis with different methods

Cited by 29 publications

References 32 publications

Effects of combined ultrasonic and microwave vacuum drying on drying characteristics and physicochemical properties of Tremella fuciformis

Effects of combined ultrasonic and microwave vacuum drying on drying characteristics and physicochemical properties of Tremella fuciformis

Physicochemical and rheological properties of Tremella fuciformis polysaccharide fractions by ethanol precipitation

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

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