Physicochemical and biological properties of bovine-derived porous hydroxyapatite/collagen composite and its hydroxyapatite powders

Sun, Ruixue; Lv, Yao; Niu, Yurong; Zhao, Xiaohui; Cao, Dong-Shan; Tang, Jian; Xianlin, Sun; Chen, Kezheng

doi:10.1016/j.ceramint.2017.09.075

Cited by 63 publications

(56 citation statements)

References 38 publications

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“…The absorption peaks at 560 and 600 cm −1 are attributed to υ4 phosphate modes [31]. The peaks at 630 and 3569 cm −1 from S1 should be assigned to hydroxyl groups (OH − ) [32]. With the increasing concentration of partially hydrolyzed keratin, the intensity of these two peaks becomes weak.…”

Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Biomimetic Hydroxyapatite Nanocrystals by Using Partially Hydrolyzed Keratin as Template Agent

et al. 2019

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Hydroxyapatite (HA), a typical inorganic component of bone, is a widely utilized biomaterial for bone tissue repair and regeneration due to its excellent properties. Inspired by the recent findings on the important roles of protein in biomineralization and natural structure of fish scales, keratin was chosen as a template for modulating the assembly of HA nanocrystals. A series of HA nanocrystals with different sizes were synthesized by adjusting the concentration of partially hydrolyzed keratin. The structure and compositions of the prepared HA were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Raman spectrum, and Transmission electron microscopy (TEM). Results revealed that the size of the synthesized HA nanocrystals can be controlled by adjusting the concentration of partially hydrolyzed keratin. Specifically, the size of synthesized HA decreased from 63 ± 1.5 nm to 27 ± 0.9 nm with the increasing concentration of partially hydrolyzed keratin from 0 to 0.6g. In addition, in vitro cytocompatibility of synthesized HA nanocrystals were evaluated using the MG-63 cells.

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Section: Resultsmentioning

confidence: 99%

Preparation and Characterization of Biomimetic Hydroxyapatite Nanocrystals by Using Partially Hydrolyzed Keratin as Template Agent

et al. 2019

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“…Another important aspect of the growing interest of using BioHA materials is related to the presence of trace elements and functional groups, which modify the chemical formula of the natural HA in bone. While the chemically-synthesized HA lacks these elements, mammalian bones contain a higher source of ions and trace elements [49][50][51], with Na + and Mg 2+ as the most frequently found ones [29]. One notes that, the presence of these ions alongside HA plays an important role in the development of teeth and bone, whereas their absence could cause fragility or bone loss [6].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of Bone Enhancement in the Case of 3D-Printed Implants Functionalized with Lithium-Doped Biological-Derived Hydroxyapatite Coatings: A Preliminary Study on Rabbits

et al. 2020

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We report on biological-derived hydroxyapatite (HA, of animal bone origin) doped with lithium carbonate (Li-C) and phosphate (Li-P) coatings synthesized by pulsed laser deposition (PLD) onto Ti6Al4V implants, fabricated by the additive manufacturing (AM) technique. After being previously validated by in vitro cytotoxicity tests, the Li-C and Li-P coatings synthesized onto 3D Ti implants were preliminarily investigated in vivo, by insertion into rabbits’ femoral condyles. The in vivo experimental model for testing the extraction force of 3D metallic implants was used for this study. After four and nine weeks of implantation, all structures were mechanically removed from bones, by tensile pull-out tests, and coatings’ surfaces were investigated by scanning electron microscopy. The inferred values of the extraction force corresponding to functionalized 3D implants were compared with controls. The obtained results demonstrated significant and highly significant improvement of functionalized implants’ attachment to bone (p-values ≤0.05 and ≤0.00001), with respect to controls. The correct placement and a good integration of all 3D-printed Ti implants into the surrounding bone was demonstrated by performing computed tomography scans. This is the first report in the dedicated literature on the in vivo assessment of Li-C and Li-P coatings synthesized by PLD onto Ti implants fabricated by the AM technique. Their improved mechanical characteristics, along with a low fabrication cost from natural, sustainable resources, should recommend lithium-doped biological-derived materials as viable substitutes of synthetic HA for the fabrication of a new generation of metallic implant coatings.

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“…thus creating the appropriate binding sites [50,51]. These spaces cause chelation between collagen and calcium ions (Ca 2+ ), with the binding sites of collagen acting as the polydentate ligand and the calcium ions as the central atom.…”

Section: X-ray Diffraction Analysismentioning

confidence: 99%

“…Collagen fibrils provide suitable sites for HAp nucleation, since collagen's structure allows for the formation of binding positions. One of the three amino acid chains of collagen, ends with an amine group which does not form a bond with the carboxylic group of the following chain, thus creating the appropriate binding sites [50,51]. These spaces cause chelation between collagen and calcium ions (Ca 2+ ), with the binding sites of collagen acting as the polydentate ligand and the calcium ions as the central atom.…”

Section: Scanning Electron Microscopy Analysismentioning

confidence: 99%

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

et al. 2020

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Bioinspired scaffolds mimicking natural bone-tissue properties holds great promise in tissue engineering applications towards bone regeneration. Within this work, a way to reinforce mechanical behavior of bioinspired bone scaffolds was examined by applying a physical crosslinking method. Scaffolds consisted of hydroxyapatite nanocrystals, biomimetically synthesized in the presence of collagen and l-arginine. Scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), microcomputed tomography, and nanoindentation. Results revealed scaffolds with bone-like nanostructure and composition, thus an inherent enhanced cytocompatibility. Evaluation of porosity proved the development of interconnected porous network with bimodal pore size distribution. Mechanical reinforcement was achieved through physical crosslinking with riboflavin irradiation, and nanoindentation tests indicated that within the experimental conditions of 45% humidity and 37 °C, photo-crosslinking led to an increase in the scaffold’s mechanical properties. Elastic modulus and hardness were augmented, and specifically elastic modulus values were doubled, approaching equivalent values of trabecular bone. Cytocompatibility of the scaffolds was assessed using MG63 human osteosarcoma cells. Cell viability was evaluated by double staining and MTT assay, while attachment and morphology were investigated by SEM. The results suggested that scaffolds provided a cell friendly environment with high levels of viability, thus supporting cell attachment, spreading and proliferation.

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Physicochemical and biological properties of bovine-derived porous hydroxyapatite/collagen composite and its hydroxyapatite powders

Cited by 63 publications

References 38 publications

Preparation and Characterization of Biomimetic Hydroxyapatite Nanocrystals by Using Partially Hydrolyzed Keratin as Template Agent

Preparation and Characterization of Biomimetic Hydroxyapatite Nanocrystals by Using Partially Hydrolyzed Keratin as Template Agent

In Vivo Assessment of Bone Enhancement in the Case of 3D-Printed Implants Functionalized with Lithium-Doped Biological-Derived Hydroxyapatite Coatings: A Preliminary Study on Rabbits

Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

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