Physicochemical Analysis of Yogurt Produced by Leuconostoc mesenteroides H40 and Its Effects on Oxidative Stress in Neuronal Cells

Abstract

Leuconostoc mesenteroides H40 (H40) was isolated from kimchi, and its probiotic properties and neuroprotective effect was evaluated in oxidatively stressed SH-SY5Y cells. H40 was stable in artificial gastric conditions and can be attached in HT-29 cells. In addition, H40 did not produce β-glucuronidase and showed resistant to several antibiotics. The conditioned medium (CM) was made using HT-29 cells refined with heat-killed probiotics (Probiotics-CM) and heated yogurts (Y-CM) to investigate the neuroprotective effect. Treatment with H40-CM not only increased cell viability but also significantly improved brain derived neurotropic factor ( BDNF ) expression and reduced the Bax/Bcl-2 ratio in oxidatively stress-induced SH-SY5Y cells. Besides, probiotic Y-CM significantly increased BDNF mRNA expression and decreased Bax/Bcl-2 ratio. The physicochemical properties of probiotic yogurt with H40 was not significantly different from the control yogurt. The viable cell counts of lactic acid bacteria in control and probiotic yogurt with H40 was 8.66 Log CFU/mL and 8.96 Log CFU/mL, respectively. Therefore, these results indicate that H40 can be used as prophylactic functional dairy food having neuroprotective effects.

Fractions of traditionally brewed rice beverage relieve anxiety and improve spatial memory in mice

“…Other bacteria like Enterobacteriaceae, Leuconostoc, Staphylococcus, Lactococcus, and Lactobacillus have been reported in different fermented foods with health benefits [41]. Furthermore, Leuconostoc is another probiotic bacterium which has been reported to demonstrate neuroprotective activity by improving the brain-derived neurotropic factor (BDNF) [42]. Furthermore, several species of Lactobacillus, Lactococcus, and Streptococcus have been extensively studied for their probiotic and brain modulatory effects.…”

Neuroprotective Effects of Heat-Killed Levilactobacillus brevis KU15152 on H2O2-Induced Oxidative Stress

“…L. brevis KU15159-CM (53.90%) and L. brevis KU15176-CM (53.93%) showed similar cell viability as H 2 O 2 -treated cells. Lee et al [29] demonstrated that the CM of Leuconostoc mesenteroides H40 attenuated cell death against H 2 O 2 in SH-SY5Y cells via the MTT assay. These results show that L. brevis KU15152 applies a neuroprotective effect against H 2 O 2 -induced toxicity.…”