Physico-chemical properties of surfactant and protein films

Patino, Juan M. Rodrı́guez; Niño, Ma. Rosario Rodríguez; Sánchez, Cecilio Carrera

doi:10.1016/j.cocis.2007.06.003

Cited by 57 publications

(20 citation statements)

References 57 publications

(33 reference statements)

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“…The heterogeneity of BLES-albumin films observed here is also in good agreement with previous studies of mixed surfactant-protein films (53,57,61). For example, de Souza et al (61) showed that addition of albumin in nanomolar concentrations can significantly increase the roughness of LB films of PL.…”

Section: Phospholipid-protein Interactions: From General Physicochemisupporting

confidence: 92%

“…The interfacial molecular interactions between chemical surfactants and proteins in general and between PL and albumin in particular are well-documented because of their importance in many biomedical and biotechnological applications, especially in food colloidal systems (51)(52)(53). The interactions between proteins and PL monolayers are dependent on the electrostatic charges of both the lipids and the proteins and the p of monolayers.…”

Section: Phospholipid-protein Interactions: From General Physicochemimentioning

confidence: 99%

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Atomic Force Microscopy Studies of Functional and Dysfunctional Pulmonary Surfactant Films, II: Albumin-Inhibited Pulmonary Surfactant Films and the Effect of SP-A

Zuo

Tadayyon

Keating

et al. 2008

Biophysical Journal

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Pulmonary surfactant (PS) dysfunction because of the leakage of serum proteins into the alveolar space could be an operative pathogenesis in acute respiratory distress syndrome. Albumin-inhibited PS is a commonly used in vitro model for studying surfactant abnormality in acute respiratory distress syndrome. However, the mechanism by which PS is inhibited by albumin remains controversial. This study investigated the film organization of albumin-inhibited bovine lipid extract surfactant (BLES) with and without surfactant protein A (SP-A), using atomic force microscopy. The BLES and albumin (1:4 w/w) were cospread at an air-water interface from aqueous media. Cospreading minimized the adsorption barrier for phospholipid vesicles imposed by preadsorbed albumin molecules, i.e., inhibition because of competitive adsorption. Atomic force microscopy revealed distinct variations in film organization, persisting up to 40 mN/m, compared with pure BLES monolayers. Fluorescence confocal microscopy confirmed that albumin remained within the liquid-expanded phase of the monolayer at surface pressures higher than the equilibrium surface pressure of albumin. The remaining albumin mixed with the BLES monolayer so as to increase film compressibility. Such an inhibitory effect could not be relieved by repeated compression-expansion cycles or by adding surfactant protein A. These experimental data indicate a new mechanism of surfactant inhibition by serum proteins, complementing the traditional competitive adsorption mechanism.

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Section: Phospholipid-protein Interactions: From General Physicochemisupporting

confidence: 92%

Section: Phospholipid-protein Interactions: From General Physicochemimentioning

confidence: 99%

Atomic Force Microscopy Studies of Functional and Dysfunctional Pulmonary Surfactant Films, II: Albumin-Inhibited Pulmonary Surfactant Films and the Effect of SP-A

Zuo

Tadayyon

Keating

et al. 2008

Biophysical Journal

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“…The physicochemical properties of emulsions prepared with these molecules have been previously reported. 32,33 Curcumin was solubilized in soy oil at 40 mg kg À1 , with or without addition of piperine at a 10 : 1 ratio (4 mg kg À1 ). The soy oil phase was heated (approx.…”

Section: Emulsion Preparationmentioning

confidence: 99%

Effect of interfacial composition on uptake of curcumin–piperine mixtures in oil in water emulsions by Caco-2 cells

2014

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Encapsulation in lipid particles is often proposed as a solution to improve curcumin bioavailability. This bioactive molecule has low water solubility and rapidly degrades during digestion. In the present study, the uptake of curcumin from oil in water emulsions, prepared with two different emulsifiers, Tween 20 and Poloxamer 407, was investigated to determine the effect of interfacial composition on absorption. Piperine was added to the curcumin to limit the degradation of curcumin because it is known to inhibit β-glucuronidase activity. The emulsions were administered to Caco-2 cell cultures, which is used as a model for intestinal uptake, and the recovery of curcumin was measured. The curcumin uptake was significantly affected by the type of interface, and the extent of curcumin uptake improved significantly by piperine addition only in the case of oil-in-water emulsions stabilized by Poloxamer 407. This work provides further evidence of the importance of interfacial composition on the delivery of bioactives.

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“…Additionally, the surfactants could be depleted from the interface if the interaction in the formation of the surfactant-protein complex is stronger. The surface tension is dependent on the concentration of the surfactant, where increased surface tension is detected with decreased surfactant concentration [7]. Due to the surface-active nature of surfactants, they are often used to reduce or even prevent adsorption of proteins [8,9], either by competitive adsorption, displacement or by adsorption of mixed layers [5].…”

Section: Introductionmentioning

confidence: 99%

The influence of size, structure and hydrophilicity of model surfactants on the adsorption of lysozyme to oil–water interface—Interfacial shear measurements

Baldursdóttir

Jørgensen

2011

Colloids and Surfaces B: Biointerfaces

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Physico-chemical properties of surfactant and protein films

Cited by 57 publications

References 57 publications

Atomic Force Microscopy Studies of Functional and Dysfunctional Pulmonary Surfactant Films, II: Albumin-Inhibited Pulmonary Surfactant Films and the Effect of SP-A

Atomic Force Microscopy Studies of Functional and Dysfunctional Pulmonary Surfactant Films, II: Albumin-Inhibited Pulmonary Surfactant Films and the Effect of SP-A

Effect of interfacial composition on uptake of curcumin–piperine mixtures in oil in water emulsions by Caco-2 cells

The influence of size, structure and hydrophilicity of model surfactants on the adsorption of lysozyme to oil–water interface—Interfacial shear measurements

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