Effect of interfacial composition on uptake of curcumin–piperine mixtures in oil in water emulsions by Caco-2 cells

Abstract

Encapsulation in lipid particles is often proposed as a solution to improve curcumin bioavailability. This bioactive molecule has low water solubility and rapidly degrades during digestion. In the present study, the uptake of curcumin from oil in water emulsions, prepared with two different emulsifiers, Tween 20 and Poloxamer 407, was investigated to determine the effect of interfacial composition on absorption. Piperine was added to the curcumin to limit the degradation of curcumin because it is known to inhibit β-glucuronidase activity. The emulsions were administered to Caco-2 cell cultures, which is used as a model for intestinal uptake, and the recovery of curcumin was measured. The curcumin uptake was significantly affected by the type of interface, and the extent of curcumin uptake improved significantly by piperine addition only in the case of oil-in-water emulsions stabilized by Poloxamer 407. This work provides further evidence of the importance of interfacial composition on the delivery of bioactives.

Submicron complex lipid carriers for curcumin delivery to intestinal epithelial cells: Effect of different emulsifiers on bioaccessibility and cell uptake

“…Other studies on the uptake of curcumin in O/W emulsions clearly showed that, when curcumin was encapsulated in Tween 20-stabilized emulsions, it was efficiently absorbed by Caco-2 cells, probably because polysorbate 20 might have created micellar structures with enhanced permeation capability (Gulseren et al, 2014). Interestingly, curcumin uptake after 2 h was comparable to what observed in HT29 cells in the present study.…”

“…However, the use of a different emulsifier, such as Poloxamer 407 resulted in significantly different uptake patterns and lower values (Gulseren et al, 2014). Similarly, the uptake of curcumin encapsulated in WPI and WPI-MS systems exhibited significantly lower profiles and slower kinetics.…”

Curcumin- and Piperine-Loaded Emulsomes as Combinational Treatment Approach Enhance the Anticancer Activity of Curcumin on HCT116 Colorectal Cancer Model

“…Higher fluorescence intensity of CurcuEmulsomes in the presence of PiperineEmulsomes was attributed to occurrence of a higher uptake. These findings is parallel to the previous studies reporting that the extent of curcumin uptake is improved significantly by piperine addition (Gülseren et al, 2014;Tang et al, 2017). Likewise, Lund and Pantuso demonstrated that addition of piperine resulted in a 229% increase in permeability of curcumin across intact Caco-2 monolayers (Lund and Pantuso, 2014).…”

The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update

“…In a recent study, its uptake was improved significantly by piperine addition only in the case of oil-in-water emulsions stabilized by Poloxamer 407 (Gulseren et al, 2014).In addition, enhanced solubility and bioavailability of curcumin was reported by a new curcumin dripping pills (Cur-DPs) formulation (Hu et al, 2015).…”