Physico‐chemical properties and <i>in vitro</i> response of silk fibroin from various domestic races

Kaewprasit, Kanyaluk; Promboon, Amornrat; Kanokpanont, Sorada; Damrongsakkul, Siriporn

doi:10.1002/jbm.b.33142

Cited by 17 publications

(27 citation statements)

References 43 publications

(52 reference statements)

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“…Fluorescence spectroscopy allows the investigation of the hydrophilic–hydrophobic interactions of proteins by tracing the localization of the tryptophan residues. Approximately 0.2–0.5% of tryptophan is present (Kaewprasit, Promboon, Kanokpanont, & Damrongsakkul, ) and dispersedly located in the linkers between crystalline regions of the SF chains (Zhou et al, ). This enables a study of the micro‐environment changes of SF during structural transitions.…”

Section: Resultsmentioning

confidence: 99%

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Laomeephol

Guedes

Ferreira

et al. 2019

J Tissue Eng Regen Med

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Silk fibroin (SF) hydrogels can be obtained via self-assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2-dimyristoyl-sn-glycero-3-phospho-(1′-rac-glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS-2, and CaSki, were encapsulated into the hydrogel. The silk-based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility.Interestingly, an inhibition in the growth of cancer-derived cell lines was observed.Therefore, DMPG-induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration.Bombyx mori "Nangnoi Srisaket 1" Thai silk cocoons were obtained from Queen Sirikit Sericulture Center, Srisaket province, Thailand.DMPG and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) were purchased from Avanti Lipids Polar, USA. Cell culture medium and all other reagents were purchased from Thermo Fisher Scientific.All reagents were of analytical grade. | SF solution preparation and its zeta potential determinationSF solution was prepared using the method adapted from Kim, Park, Joo Kim, Wada, and Kaplan (2005). In brief, silk cocoons were boiled in 0.02 M Na 2 CO 3 for 20 min, followed by their thoroughly washing with distilled water. The extracted silk fibre was then dissolved in 9.3 M LiBr at 60°C for 4 hr. The silk solution was dialysed against distilled water using a dialysis tube (MWCO 12-16 kDa; Sekisui, Japan) for 48 hr. The final concentration was approximately 6%, which was determined from dried solid weight.Analysis of zeta potential of the SF solution was conducted using laser doppler electrophoresis in a Zetasizer Nano ZS equipment (Malvern Instruments) at 25°C.

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Section: Resultsmentioning

confidence: 99%

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Laomeephol

Guedes

Ferreira

et al. 2019

J Tissue Eng Regen Med

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“…Due to the presence of amino group containing residues as well as a high content of tyrosine in SF (~5.75%) [15], it can be used in the production of AuNPs by a simple procedure and without hazardous reagents [11,16]. Moreover, the preparation of hydrogels using thiolated polymers (e.g., thiolated polyethylene glycol) crosslinked by Au 3+ was previously reported [17,18].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Gelation Mechanisms and Cytocompatibility of Gold (III)-Mediated Regenerated and Thiolated Silk Fibroin Hydrogels

et al. 2020

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Accelerating the gelation of silk fibroin (SF) solution from several days or weeks to minutes or few hours is critical for several applications (e.g., cell encapsulation, bio-ink for 3D printing, and injectable controlled release). In this study, the rapid gelation of SF induced by a gold salt (Au3+) as well as the cytocompatibility of Au3+-mediated SF hydrogels are reported. The gelation behaviors and mechanisms of regenerated SF and thiolated SF (tSF) were compared. Hydrogels can be obtained immediately after mixing or within three days depending on the types of silk proteins used and amount of Au3+. Au3+-mediated SF and tSF hydrogels showed different color appearances. The color of Au-SF hydrogels was purple-red, whereas the Au-tSF hydrogels maintained their initial solution color, indicating different gelation mechanisms. The reduction of Au3+ by amino groups and further reduction to Au by tyrosine present in SF, resulting in a dityrosine bonding and Au nanoparticles (NPs) production, are proposed as underlying mechanisms of Au-SF gel formation. Thiol groups of the tSF reduced Au3+ to Au+ and formed a disulfide bond, before a formation of Au+-S bonds. Protons generated during the reactions between Au3+ and SF or tSF led to a decrease of the local pH, which affected the chain aggregation of the SF, and induced the conformational transition of SF protein to beta sheet. The cytocompatibility of the Au-SF and tSF hydrogels was demonstrated by culturing with a L929 cell line, indicating that the developed hydrogels can be promising 3D matrices for different biomedical applications.

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“…22,29 Fibroin undergoes weight loss at *250-400°C. 29,34 The weight loss pattern of sericin has been detected in a wide temperature range starting at 220°C. 35,36 The DSC results in this study (Fig.…”

Section: Discussionmentioning

confidence: 99%

Fibroin and Polyvinyl Alcohol Hydrogel Wound Dressing Containing Silk Sericin Prepared Using High-Pressure Carbon Dioxide

Napavichayanun

Bonani

Yang

et al. 2019

Advances in Wound Care

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Objective: To fabricate and investigate the properties of fibroin and polyvinyl alcohol (PVA) hydrogels containing sericin prepared using high-pressure carbon dioxide (CO 2). Approach: In this study, fibroin/PVA hydrogels with and without sericin were prepared using the high-pressure CO 2 method. The physical and mechanical properties of the hydrogels were investigated using field-emission scanning electron microscopy, Fourier-transform infrared spectroscopy, thermogravimetric analysis, and differential scanning calorimetry, and the swelling, water retention, and compressive properties were assessed. Results: The hydrogels obtained from the combination of fibroin and PVA presented a compositional gradient along the hydrogel thickness and structure. The upper layer of the hydrogel consisted of a fibroin-based hydrogel blended with PVA, whereas the lower layer contained only fibroin. The mechanical properties regarding compression of the fibroin/PVA hydrogel were significantly better than those of the pure fibroin hydrogel, for hydrogels with and without sericin. Moreover, the mechanical properties of the hydrogels with sericin were significantly better than those without sericin. The water contents of all samples were >90%. Innovation: This study assessed a new combination of a wound healing agent and a biomaterial dressing. Moreover, this hydrogel production technique used a clean method without the need for a chemical crosslinking agent. Conclusion: The combination of the fibroin and PVA hydrogel and sericin prepared using the high-pressure CO 2 method led to good physical properties. This material may be a candidate for medical applications.

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Physico‐chemical properties and in vitro response of silk fibroin from various domestic races

Cited by 17 publications

References 43 publications

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration

Exploring the Gelation Mechanisms and Cytocompatibility of Gold (III)-Mediated Regenerated and Thiolated Silk Fibroin Hydrogels

Fibroin and Polyvinyl Alcohol Hydrogel Wound Dressing Containing Silk Sericin Prepared Using High-Pressure Carbon Dioxide

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