This study aimed to determine physicochemical properties (PV, FFA, total phenolic content, antioxidant activity), fatty acid, sterol, tocopherol and phenolic compound profile of sweet cherry seed oil. Sweet cherry oil was obtained by cold press process. Tocopherol and phenolic compounds were determined by HPLC and fatty acid and sterol composition were analyzed by GC system. PV and FFA values were found as 1.40 meqO2/kg and 2.71 % respectively. Apparent viscosity value was 0.057 Pa.s. TPC and AC values of the sweet cherry oil were determined as 22.17 mg GAE/g of extract and 1.05 mmol TE/g of extract. UFA content was higher than that of the SFA. SFA and UFA levels were 10.53% and 89.47 % respectively. MUFA (37.44 %) level was lower than PUFA level (52.03 %). Oleic acid and linoleic acid were found to be major fatty acids with a ratio of 36.73 % and 39.45 %, respectively. 8 different sterols were analyzed for sweet cherry seed oil. -sitosterol was found to be the major sterol with a ratio of 88.93 % followed by campesterol (3.12 %), 7-stigmasterol (2.48 %), 5-avenasterol (2.12 %) and sitostanol (1.42 %). Other sterols showed small amount (<1%). Total four tocopherols namely, , , and δ tocopherols were identified for sweet cherry oils. -tocopherols (96.72 mg /kg) were found as major tocopherol followed by -tocopherols (57.40 mg/kg) and δ tocopherols. -tocopherol showed a small amount. This study suggested that sweet cherry seed oil can be utilized in food industry due to high level of bioactive compounds and low levels of PV and FFA content.