<p>Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats</p>

Abstract

Background: Peptic ulcer disease, a painful lesion of the gastric mucosa, is considered one of the most common gastrointestinal disorders. This study aims to investigate the formulation of pumpkin seed oil (PSO)-based nanostructured lipid carriers (NLCs) to utilize PSO as the liquid lipid component of NLCs and to achieve oil dispersion in the nano-range in the stomach. Methods: Box-Behnken design was utilized to deduce the optimum formula with minimum particle size. The optimized PSO-NLCs formula was investigated for gastric ulcer protective effects in Wistar rats by evaluating ulcer index and determination of gastric mucosa oxidative stress parameters. Results: PSO was successfully incorporated as the liquid lipid (LL) component of NLCs.The prepared optimum PSO-NLCs formula showed a size of 64.3 nm. Pretreatment of animals using the optimized PSO-NLCs formula showed significantly (p< 0.001) lower ulcer index compared to indomethacin alone group and significantly (p<0.05) less mucosal lesions compared to the raw oil. Conclusion: These results indicated great potential for future application of optimized PSO-NLCs formula for antiulcer effect in non-steroidal anti-inflammatory drug (NSAID)-induced gastric ulcer.

Optimized Self-Nanoemulsifying Delivery System Based on Plant-Derived Oil Augments Alpha-Lipoic Acid Protective Effects Against Experimentally Induced Gastric Lesions

“…This finding is consistent with our previous study which reported a gastroprotective effect of pumpkin oil that has been used as a component of SNEDDs in this study. 20 This finding may be a potential for using smaller doses of ALA since the vehicle itself of ALA formula could add to the therapeutic activity of ALA against IND-induced ulcer. However, further studies will be needed to confirm this by using different doses of ALA.…”

“…Quantitative assessment of gastric mucosal lesions was done in accordance with the method described in our previous studies. 20 , 21 Stomachs were cleaned, pinned on board and pictures were taken for the stomach samples then were scored for the degree of mucosal damage using Image J software. Areas of mucosal damage were expressed as a percentage of the total surface area of the examined stomach.…”

“…MDA reacts with thiobarbituric acid (TBA) at high temperature and forms MDA-TBA adducts that can be measured calorimetrically based on the method used by Mihara and Uchiyama 25 and as described by our previous studies. 20 …”

“…Such formulations have the advantage of presenting the drug in a highly solubilized form when used for drug delivery. 20 , 21 Additionally, the small average droplet size of the produced nanoemulsion offers a large interfacial area for an enhanced absorption of the loaded therapeutics. As compared with traditional emulsions, which are sensitive dispersed forms of low stability characteristics, SNEDDSs are found to be a promising approach as they are thermodynamically stable and easy-to-produce formulations.…”

Assessment of the Potential of Sarcandra glabra (Thunb.) Nakai. in Treating Ethanol-Induced Gastric Ulcer in Rats Based on Metabolomics and Network Analysis

“…It has been reported that the occurrence of alcohol-induced GU is mainly related to oxidative stress, inflammation, and lipid peroxidation ( Kobayashi et al, 2001 ; Zhang et al, 2019 ; Ahmed et al, 2020 ). Oxidative stress is the excessive production of free radicals when the body is subjected to various harmful stimuli (radiation, ischemia, hypoxia, etc.,) which the antioxidant system cannot effectively and efficently remove, causing an imbalance between the oxidation and antioxidant system, thus leading to tissue damage ( Tan et al, 2018 ).…”