Physico-chemical characterization of collagen scaffolds for tissue engineering

León-Mancilla, Benjamín; Araiza-Téllez, M.A.; Flores-Flores, José O.; Piña-Barba, María C.

doi:10.1016/j.jart.2016.01.001

Cited by 81 publications

(63 citation statements)

References 14 publications

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“…According to TGA thermogram, nearly 10% weight loss by 100 o C can be corresponded to moisture loss and chemical water in the cryogel. The main weight losses occurred between 100 o C-250 o C and 250 o C-550 o C. These findings are similar with the literature [25,26]. The final residue after mass decomposition can be associated with ashes and undecomposed material.…”

Section: Characterization Of Cryogelssupporting

confidence: 90%

Functional Polysaccharides Blended Collagen Cryogels

Odabaş

2018

HJBC

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A raştırmacılar doku mühendisliği ve rejeneratif tıp uygulamalarında, hücre çoğalması ve büyümesi, doku hasarının onarılmasını ve yenilenmesini desteklemek için yeni doku iskeleleri araştırmaları yapmaktadırlar. Günümüzde çok sayıda farklı polimerik malzeme ve değişik hazırlama teknikleri kullanılsa da, henüz hangi doku iskelesinin doku tamiri ve yenilenmesi için en iyidir? sorusunun cevabı için bir uzlaşı yoktur. Hidrojellerin bir türü olan kriyojeller ve kriyojel hazırlama tekniği olan kriyojelasyon, doku mühendisliği uygulamaları açısından kısmen yeni sayılırlar. Bu çalışmada, karboksimetil selüloz ve dekstran içeren fonksiyonel polisakkarit içeren kollajen kriyojeller hazırlanmış ve kimyasal, yapısal ve biyolojik değerlendirmeler ile karakterize edilmiştir. Sonuçlar, hazırlanan fonksiyonel polisakkarit birimleri içeren kollajen kriyojellerin kimyasal ve termal olarak uygun özelliklerinin yanında biyo ve kan uyumluluklarının iyi olduğunu ortaya koyulmuştur. Sonuç olarak, bu kriyojeller doku mühendisliği ve rejeneratif tıp uygulamaları için uygun bir aday doku iskelesi olarak kullanılabilirler. Anahtar KelimelerKollajen, polisakkaritler, kriyojeller, doku mühendisliği. A B S T R A C T R esearches investigate new types of scaffolds for tissue engineering and regenerative medicine applicationsto support cell proliferation and growth as well as tissue repair and regeneration. Although, there are several types of polymeric materials and various kinds of preparation techniques are already used today; there is still no consensus on the answer of the question "what is the best scaffold for tissue repair and regeneration?". Cryogels, which is a kind of hydrogels and, cryogelation, which is the technique for cryogel preparation, are rather new for tissue engineering applications. Here in this study, dextran and carboxymethyl cellulose functional polysaccharides blended collagen cryogels were prepared and characterized by chemical, structural and biological evaluations. Results show that, these collagen cryogels with their polysaccharides functional components have proper chemical and thermal characteristics and show good bio and hemocompatibility. Therefore, these cryogels can be used as a candidate scaffold for tissue engineering and regenerative medicine applications.

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Section: Characterization Of Cryogelssupporting

confidence: 90%

Functional Polysaccharides Blended Collagen Cryogels

Odabaş

2018

HJBC

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“…). The FTIR spectrum of the collagen scaffold exhibited peaks at 1236, 1542, 1643, 3063, and 3295 cm −1 attributable to the amide III, II, I, B, and A functional groups, respectively . For the composite scaffold, peaks observed at 660 and 1008 cm −1 were attributable to the

{PO}_{4}^{- 3}

of β‐TCP, and those at 1547 and 1650 cm −1 were due to the functional groups of the collagen …”

Section: Resultsmentioning

confidence: 99%

Development of a bioactive porous collagen/β‐tricalcium phosphate bone graft assisting rapid vascularization for bone tissue engineering applications

Baheiraei

Nourani

Mortazavi

et al. 2017

J Biomedical Materials Res

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Abstract:We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/b-TCP) scaffolds with a b-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrowderived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was, also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats. We observed a microporous structure with large porosity (95-98%) and appropriate pore size (120-200 mm). The COL/b-TCP scaffolds had a much higher compressive modulus (970 6 1.20 KPa) than pure COL (0.8 6 1.82 KPa). In vitro model of apatite formation was established by immersing the composite scaffold in simulated body fluid for 7 days. An ALP assay revealed that porous COL/b-TCP can effectively activate the differentiation of BMMSCs into osteoblasts. The composite scaffolds also promoted vascularization with good integration with the surrounding tissue. Thus, introduction of b-TCP powder into the porous collagen matrix effectively improved the mechanical and biological properties of the collagen scaffolds, making them potential bone substitutes for enhanced bone regeneration in orthopedic and dental applications.

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“…This Specific surface area (SSA) is measured as particle total area/total weight. 30 This dehydration phase continued up to 300 C with about 0.8% and 5.3% mass loss for nHA-1 and nHA-F, respectively, while, nHA-2 displayed no changes. was compared to nHA-F and nβ-TCP which displayed 6.5% and 2.3% mass loss, respectively (Fig.…”

Section: Analysis Of Thermal Behaviormentioning

confidence: 91%

“…Phase I was characterized by an initial rapid mass loss between room temperature and 120 C which corresponded to the evaporation of loosely bound and adsorbed water molecules due to moisture/air contamination. 30 This dehydration phase continued up to 300 C with about 0.8% and 5.3% mass loss for nHA-1 and nHA-F, respectively, while, nHA-2 displayed no changes. Phase II (300-600 C) exhibited a more constant TGA curve with minor changes in the mass.…”

Section: Analysis Of Thermal Behaviormentioning

confidence: 91%

Synthesis and characterization of biomimetic bioceramic nanoparticles with optimized physicochemical properties for bone tissue engineering

Ebrahimi

Botelho

et al. 2019

J Biomedical Materials Res

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Calcium phosphate bioceramics nanoparticles such as nano‐hydroxyapatite (nHA) and nano‐tricalcium phosphate (nTCP) are the main focus of basic and applied research for bone tissue regeneration. In particular, a combination of these two phases (nHA + nTCP) which refers to as “nano‐biphasic calcium phosphates (nBCP)” is of interest due to the preferred biodegradation nature compared to single‐phase bioceramics. However, the available synthesis processes are challenging and the biomaterials properties are yet to be optimized to mimic the physiochemical properties of the natural nanoscale bone apatite. In this study, a new approach was developed for the production of optimized bioceramic nanoparticles aiming to improve their biomimecity for better biological performances. Nanoparticles were synthesized through a carefully controlled and modified wet mechano‐chemical method combined with a controlled solid‐state synthesis. Different processing variables have been analyzed including; milling parameters, post‐synthesis treatment, and calcination phase. Detailed physicochemical characterizations of nanoparticles revealed higher crystallinity (∼100%), lower crystallite/particle size (58 nm), higher homogeneity, reduced particle agglomeration size (6 μm), and a closer molar ratio (1.8) to biological apatite compared to control and standard samples. Furthermore, the study group was confirmed as calcium‐deficient carbonate‐substituted BCP nanoparticles (nHA/nβ‐TCP: 92/8%). As such, the introduced method can afford an easier and accurate control over nanoparticle physiochemical properties including the composition phase which can be used for better customization of biomaterials for clinical applications. The findings of this article will also help researchers in the further advancement of production strategies of biomaterials. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1654–1666, 2019.

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Physico-chemical characterization of collagen scaffolds for tissue engineering

Cited by 81 publications

References 14 publications

Functional Polysaccharides Blended Collagen Cryogels

Functional Polysaccharides Blended Collagen Cryogels

Development of a bioactive porous collagen/β‐tricalcium phosphate bone graft assisting rapid vascularization for bone tissue engineering applications

Synthesis and characterization of biomimetic bioceramic nanoparticles with optimized physicochemical properties for bone tissue engineering

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