Physico-chemical changes of ZnO nanoparticles with different size and surface chemistry under physiological pH conditions

Gwak, Gyeong‐Hyeon; Lee, Won–Jae; Paek, Seung‐Min; Oh, Jae‐Min

doi:10.1016/j.colsurfb.2015.01.021

Cited by 15 publications

(13 citation statements)

References 24 publications

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“…However, particle size did not affect solubility, as demonstrated by dissolutions of ~24%, 0.2%, and 2.8% in simulated gastric fluid, intestinal fluid, and plasma, respectively, for both particle sizes ( Figure 2 ). The high dissolution properties of ZnO NPs under acidic and gastric conditions [ 28 ] and low solubility in neutral fluids have been well reported [ 29 , 30 ], which is in good agreement with our results. Further investigation on ex vivo solubility showed ~12%, ~9%, and 2% dissolution of both bulk and nano ZnOs in rat-extracted gastric, intestinal, and plasma fluids, respectively, without statistical significances between particle sizes ( Figure 3 ).…”

Section: Discussionsupporting

confidence: 93%

ZnO Interactions with Biomatrices: Effect of Particle Size on ZnO-Protein Corona

Jin

Kim

et al. 2017

Nanomaterials

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Zinc oxide (ZnO) nanoparticles (NPs) have been widely used for food fortification, because zinc is essential for many enzyme and hormone activities and cellular functions, but public concern about their potential toxicity is increasing. Interactions between ZnO and biomatrices might affect the oral absorption, distribution, and toxicity of ZnO, which may be influenced by particle size. In this study, ZnO interactions with biomatrices were investigated by examining the physicochemical properties, solubility, protein fluorescence quenching, particle–protein corona, and intestinal transport with respect to the particle size (bulk vs. nano) in simulated gastrointestinal (GI) and plasma fluids and in rat-extracted fluids. The results demonstrate that the hydrodynamic radii and zeta potentials of bulk ZnO and nano ZnO in biofluids changed in different ways, and that nano ZnO induced higher protein fluorescence quenching than bulk ZnO. However, ZnO solubility and its intestinal transport mechanism were unaffected by particle size. Proteomic analysis revealed that albumin, fibrinogen, and fibronectin play roles in particle–plasma protein corona, regardless of particle size. Furthermore, nano ZnO was found to interact more strongly with plasma proteins. These observations show that bulk ZnO and nano ZnO interact with biomatrices in different ways and highlight the need for further study of their long-term toxicity.

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Section: Discussionsupporting

confidence: 93%

ZnO Interactions with Biomatrices: Effect of Particle Size on ZnO-Protein Corona

Jin

Kim

et al. 2017

Nanomaterials

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“…A variety of different constituents, pH and temperature regimes, which vary depending on the purpose of each study, have been used to assess solubility of nanomaterials. Examples include the following: phosphate buffer saline (PBS) adjusted to pH 1.2, 6.8 and 7.4 to simulate gastric, intestinal and plasma conditions respectively (Gwak et al 2015 ); NaCl/pepsin solution adjusted to pH 1.5 with HCL to simulate gastric conditions (Cho et al 2013 ); Dulbecco’s modified Eagle’s medium (DMEM), pH 7.68 and BEGM held at 37 °C (Mu et al 2014 ; Xia et al 2008 ); ALF and Gamble solution at 38 °C to simulate alveolar and interstitial lung fluid respectively (Stebounova et al 2011 ); 0.01 M Ca(NO 3 ) 2 buffered with 2 mM piperazine- N , N ′-bisethanesulfonic acid (PIPES) to pH 7.5 ± 0.1 held at 21 °C (Yin et al 2015 ). Constituents of simulated biological media, such as organic and inorganic ligands, may either increase or decrease dissolution, and therefore, the impact of media components on solubility must be understood for each ENM (David et al 2012 ; Li et al 2011 ; Mu et al 2014 ; Mudunkotuwa et al 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of pH, particle size and crystal form on dissolution behaviour of engineered nanomaterials

Avramescu

Rasmussen

Chénier

et al. 2016

Environ Sci Pollut Res

112

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Solubility is a critical component of physicochemical characterisation of engineered nanomaterials (ENMs) and an important parameter in their risk assessments. Standard testing methodologies are needed to estimate the dissolution behaviour and biodurability (half-life) of ENMs in biological fluids. The effect of pH, particle size and crystal form on dissolution behaviour of zinc metal, ZnO and TiO2 was investigated using a simple 2 h solubility assay at body temperature (37 °C) and two pH conditions (1.5 and 7) to approximately frame the pH range found in human body fluids. Time series dissolution experiments were then conducted to determine rate constants and half-lives. Dissolution characteristics of investigated ENMs were compared with those of their bulk analogues for both pH conditions. Two crystal forms of TiO2 were considered: anatase and rutile. For all compounds studied, and at both pH conditions, the short solubility assays and the time series experiments consistently showed that biodurability of the bulk analogues was equal to or greater than biodurability of the corresponding nanomaterials. The results showed that particle size and crystal form of inorganic ENMs were important properties that influenced dissolution behaviour and biodurability. All ENMs and bulk analogues displayed significantly higher solubility at low pH than at neutral pH. In the context of classification and read-across approaches, the pH of the dissolution medium was the key parameter. The main implication is that pH and temperature should be specified in solubility testing when evaluating ENM dissolution in human body fluids, even for preliminary (tier 1) screening.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-016-7932-2) contains supplementary material, which is available to authorized users.

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“…Cellular uptake of ZnO20, as analyzed by fluorescence intensity, was more than 20 times greater than that of ZnO70. According to our quantitative analyses of Cy5.5 labelling using UV-vis spectroscopy, approximately 2.5 times more Cy5.5 was conjugated on ZnO20 than on ZnO70, due to their difference in specific surface area (27.7 m 2 /g and 16.6 m 2 /g for ZnO20 and ZnO70, respectively [46]). Nevertheless, a markedly and significantly higher amount of ZnO20 as compared to ZnO70 was internalized into the HaCaT cells.…”

Section: Evaluation Of Cellular Uptake and Internalization Pathway Ofmentioning

confidence: 99%

Stable fluorescence conjugation of ZnO nanoparticles and their size dependent cellular uptake

Kim

Paek

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Physico-chemical changes of ZnO nanoparticles with different size and surface chemistry under physiological pH conditions

Cited by 15 publications

References 24 publications

ZnO Interactions with Biomatrices: Effect of Particle Size on ZnO-Protein Corona

ZnO Interactions with Biomatrices: Effect of Particle Size on ZnO-Protein Corona

Influence of pH, particle size and crystal form on dissolution behaviour of engineered nanomaterials

Stable fluorescence conjugation of ZnO nanoparticles and their size dependent cellular uptake

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