Physico-chemical and Antifungal Properties of Trypsin Inhibitor from the Seeds of Mucuna pruriens

Chandrashekharaiah, K. S.

doi:10.13005/ojc/290329

Cited by 4 publications

(3 citation statements)

References 40 publications

(26 reference statements)

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“…Fruits are used as catharatic and in snake bite. Juice of leaves is used in skin diseases [31][32][33] . Flowers and pods are used as purgative, febrifugal, biliousness and astringent.…”

Section: Identification Of Phytochemical Compoundsmentioning

confidence: 99%

In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

Kadhim¹,

Mohammed²,

Hameed³

2016

Orient. J. Chem

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The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and Pharmacological actions. GC-MS analysis of Cassia fistula revealed the presence of the Oxacyclododecan-2-one, Imidazole ,2-amino-5-[(2-carboxy)vinyl], D-Glucose , 6-O-á-D-galactopyranosyl, 2-Nonanone, Eicosanoic acid , phenylmethyl ester, Phenol , 4-(2-propenyl), Eugenol, Caryophyllene, ß-copaene, Azulene,1,2,3,3a,4,5,6, 2,4, 1a,2,5,5a,6,9,10, Vitamin E and Glycine ,5ß,. The FTIR analysis of Cassia fistula leaves proved the presence of Alkenes, Aliphatic fluoro compounds, Alcohols, Ethers, Carboxlic acids, Esters, Nitro Compounds, Alkanes, Alcohols and Phenol. Cassia fistula was highly active against Aspergillus terreus (6.99±0.29). Methanolic extract of bioactive compounds of Cassia fistula was assayed for in vitro antibacterial activity against eleven pathogenic bacteria by using the diffusion method in agar. The zone of inhibition were compared with different standard antibiotics. The diameters of inhibition zones ranged from 1.00±0.05 to 6.02±0.23 mm for all treatments.

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“…Fruits are used as catharatic and in snake bite. Juice of leaves is used in skin diseases [31][32][33] . Flowers and pods are used as purgative, febrifugal, biliousness and astringent.…”

Section: Identification Of Phytochemical Compoundsmentioning

confidence: 99%

In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

Kadhim¹,

Mohammed²,

Hameed³

2016

Orient. J. Chem

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“…The tests were carried out in triplicate. The antifungal activity was evaluated by measuring the inhibition-zone diameter observed after 48 h of incubation [39][40][41][42][43][44][45][46][47][48][49] .…”

Section: Determination Of Antibacterial Activitymentioning

confidence: 99%

Analysis of Volatile Metabolites Released by Staphylococcus Aureus using Gas Chromatography-Mass Spectrometry and Determination of its Antifungal Activity

Jaddoa¹,

Hameed²,

Mohammed³

2016

Orient. J. Chem

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Bacterial volatile organic compounds (VOCs) have been considered as sensitive and specific biomarkers for bacterial detection in human specimens and culture media. The possibility of using VOCs markers as one of the largest groups of bacterial metabolites would open a new frontier for developing more efficient techniques in the diagnosis of bacterial infections. The aims of this research were analysis of the bioactive chemical products and evaluation of antibacterial and antifungal activity. Bioactives (chemical compounds often referred to as secondary metabolites) were analyzed using gas chromatography-mass spectroscopy (GC-MS) techniques, then the in vitro antibacterial and antifungal activity of the methanolic extract was evaluated. Thirty five bioactive compounds were identified in the methanolic extract of Staphylococcus aureus. GC-MS analysis of Staphylococcus aureus revealed the existence of the: Hexanoic acid , 2-methyl, 12,15-Octadecadiynoic acid , methyl ester, 1-Aminononadecane , 2,3,4,5,6,7, Cystine,3,butylamine, 3-methoxy-2-(1-methylethyl)-5-(2-methylpropyl)pyrazine, Uric acid, Thiocyanic acid 4-methoxy-2,6-dimethyl-3-pyridyl ester, 12-Dimethylamino-10-oxododecanoic acid, Glycyl-D-asparagine, Actinomycin C2, 12-Octadecenoic acid , methyl ester, 6-Octadecenoic acid , methyl ester ,(z)-, 2,5-Piperazinedione , 3,6-bis(2-methylpropyl)-, l-Leucyl-d-leucine and Methyl 12-hydroxy-9-octadecenoate. Gramineae poaceae was very highly active (6.71±0.13) mm. The results of anti-fungal activity produced by Staphylococcus aureus showed that the volatile compounds were highly effective to suppress the growth of Aspergillus terreus. Staphylococcus aureus produce many important secondary metabolites with high biological activities. Based on the significance of employing bioactive compounds in pharmacy to produce drugs for the treatment of many diseases, the purification of compounds produced by Staphylococcus aureus can be useful.

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“…2 This plant is a natural source of L-3,4dihydroxyphenyl alanine (L-DOPA), a neurotransmitter precursor which is effective against Parkinson's disease. 3 The seeds of M. pruriens are considered as astringent, aphrodisiac, nervine and have a high nutritional value which showed trypsin inhibitory activity 4 , hepatoprotective activity and antioxidant properties. 5 Anti-inflammatory, diuretic and antibacterial activities of the aerial parts of DOI: https://doi.org/10.3329/dujps.v20i1.54038 M. pruriens have been already reported.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis, Antioxidant and Cytotoxic Activities of Mucuna pruriens Leaves

Ferdous

Rahman

Ahmed

et al. 2021

Dhaka Univ. J. Pharm. Sci

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Mucuna pruriens is a tropical legume native to Africa, India and Bangladesh and is widely cultivated in tropical countries. In this study, a crude methanolic extract of the leaves of M. pruriens was investigated for its chemical constituents and to explore the phenolic and flavonoid content, antioxidant, cytotoxic and antimicrobial activities using established protocols. From the ethyl acetate soluble fraction of the crude methanol extract, three known compounds namely ferulic acid (1), 2-(5-methoxy-1-benzofuran-3-yl)-N-ethylethanamine (2) and stizolamine (3) were isolated and their structures were elucidated by the analysis of NMR spectral data. The crude extract was found to possess phenolic content of 216.16 μg/g whereas the concentration of flavonoid was found to 214.8 μg/g expressed in quercetin standard. Free radicals generated through DPPH were neutralized by crude methanolic extract and the IC50 value was obtained as 19.63 μg/ml. Regression analysis during brine shrimp lethality test enumerated LC50 value of crude methanolic extract at 10.72 μg/ml and was significant compared to the positive standard. The crude methanolic extract of leaf of M. pruriens did not show any significant antimicrobial activity against the organisms used in our test. Dhaka Univ. J. Pharm. Sci. 20(1): 103-109, 2021 (June)

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Physico-chemical and Antifungal Properties of Trypsin Inhibitor from the Seeds of Mucuna pruriens

Cited by 4 publications

References 40 publications

In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

Analysis of Volatile Metabolites Released by Staphylococcus Aureus using Gas Chromatography-Mass Spectrometry and Determination of its Antifungal Activity

Phytochemical Analysis, Antioxidant and Cytotoxic Activities of Mucuna pruriens Leaves

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