Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

Huang, Ya-Ling; Tsai, Yi-Jung; Cheng, T.-C.; Chen, J.-J.; Chen, F.-C.

doi:10.4238/2014.november.12.3

Cited by 11 publications

(10 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The seeds were then sterilized with 0.6% sodium hypochlorite solution for 10 min then washed 3 times with sterile water. Later the sterilized seeds were sown on a germination medium containing Hyponex (7N-6P 2 O 5 -19K 2 O) plus 1 g l −1 Bacto-tryptone, 50 g l −1 potato homogenate, 50 g l −1 banana homogenate, 30 g l −1 sucrose, 2 g l −1 activated charcoal, and 7.5 g l −1 agar as reported previously (Huang et al 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

et al. 2018

View full text Add to dashboard Cite

BackgroundIn a breeding program, usually only superior parents are chosen for cross hybridization. Pollens of elite cultivars may not be available at hand. Properly stored pollens provide an opportunity for cross hybridization at unavailable time.ResultsPollen of a Phalaenopsis hybrid was evaluated for the storage ability at different temperatures, including room temperature, 4, − 20, and − 80 °C for up to 96 weeks. The viability of pollen was assessed by TTC staining, in vitro germination and hand pollination during and after storage. Pollen stored at all temperatures for 4 weeks remained viable and capable of successful pollination. Pollen lost its viability after 4 weeks at room temperature. Pollen remains viable after 40 weeks at 4 °C, and after 96 weeks at both − 20 and − 80 °C of storage. Viable pollen could be successfully pollinated to the female parent at all effective storage conditions and produced seeds.ConclusionsOur results indicate that Phalaenopsis pollen can be stored at 4 °C up to 40 weeks for short-term purpose. For long-term storage, pollen can be kept at both − 20 and −80 °C.

show abstract

Section: Methodsmentioning

confidence: 99%

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

et al. 2018

View full text Add to dashboard Cite

show abstract

“…2) after removal of the primary PLB apical parts to prevent apical dominance. Huang, Tsai, Cheng, Chen, & Chen (2014) have reported the formation of many secondary PLB structures in the epidermal layer around the excised PLBs. The addition of TDZ to the PLB proliferation medium did not show any significant effect on all parameters during the PLB proliferation (Table 3).…”

Section: Effects Of Tdz and Pvp On Plb Proliferationmentioning

confidence: 99%

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

et al. 2018

View full text Add to dashboard Cite

Phalaenopsis amabilis, commonly known in Indonesia as 'Anggrek Bulan' is one of the Orchidaceae species typically having large (7-12 cm), pure white flowers with yellow coloration and some red stripes on its labellum (Handoyo, 2010). Based on their perianth morphology, there are six types of Phal. amabilis, such as Taiwan, Sumatera, Irian Jaya, Java, Borneo, and Grandiflora types. The Phal. amabilis from Sumatera has smaller perianth than those from Java and Borneo while Phal. grandiflora from Borneo is characterized as having the largest flower and more than 5 cm petals (Ikedo, n.d.). As in many orchid species, illegal harvest, forest destruction, and climatic changes may contribute to the decline of their natural populations (Crain & Tremblay, 2014; Crain & Tremblay, 2017; Fay, 2018). Therefore, it is necessary to develop concerted efforts for ex situ conservation of endangered orchid species before it becomes extinct. The development of ex-situ conservation requires the use of tissue culture as the alternative technology for clonal propagation (Merritt, Hay, Swarts, Sommerville, & Dixon, 2014). Various tissues and organs have been used as explants to initiate propagation of Phalaenopsis, such as leaf (Balilashaki, Naderi, Kalantari,

show abstract

“…In addition, Ng and Saleh (2011) suggested that PGR-free medium could obtain genetically stable PLBs. In recent years, secondary PLB development from PLBs has been shown for various orchids, including Dendrobium (Julkiflee et al, 2014), Doritaenopsis (Amaki and Higuchi, 1989), and Phalaenopsis (Huang et al, 2014;Khoddamzadeh et al, 2011). However, the results in this study showed that both large and small PLBs formed secondary PLBs (PLB proliferation) at nearly the same rates over 2, 4, and 6 weeks of culture (Fig.…”

Section: Resultsmentioning

confidence: 50%

“…This may be because the whole PLB and the upper side of the PLB still had many cell clumps around apical meristems that it would protrude and develop after 2 weeks. Others have shown that the lower halves of bisected PLBs are more efficient in the proliferation of secondary PLBs (Amaki and Higuchi, 1989;Huang et al, 2014;Tanaka, 1987).…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Tolumnia Orchids through Induction of Protocorm-like Bodies from Leaf Segments

Chookoh¹,

Chiu²,

Chang³

et al. 2019

horts

View full text Add to dashboard Cite

A protocol for plant regeneration via direct induction of protocorm-like bodies (PLBs) from leaf segments of Tolumnia Snow Fairy was developed as a basis for mass production. Ten-month-old, in vitro–grown donor plantlets were obtained by inducing shoots from buds on the flower stalk. Leaf segments harvested from plantlets of different heights and from expanding leaves at different positions were compared, as were two BA concentrations with 0.5 mg·L−1 NAA. The greatest rate of PLB induction (16.7%) was observed when leaf segments taken from 1- to 2-cm height plants were cultured in Murashige and Skoog (MS) basal medium supplemented with 2 mg·L−1 BA and 0.5 mg·L−1 NAA after 16 weeks of culture. When using leaf explants, only inner, expanding leaves cultured on MS basal medium supplemented with 4 mg·L−1 BA and 0.5 mg·L−1 NAA resulted in PLB induction, at an average rate of 25.5 PLBs per explant. After 16 weeks of culture, histological and scanning electron microscopy (SEM) observations revealed that PLBs originated from epidermal cells of leaf explants. PLBs of 1 to ≤2 mm in diameter continued to proliferate after 4 weeks of culture. These secondary PLBs could be produced from either whole PLBs or the upper side of PLBs. Finally, PLBs were regenerated into plantlets. After ≈14 months of culture, fully developed plants exhibiting well-developed roots and shoots were acclimatized. These plants grew well, with 1-year survival rates of nearly 73%, for plants originating as explants taken from 1- to 2-cm tall plants, and of 79%, for plants originating as explants taken from inner leaves. Some mature plants flowered 1 year after transplantation. This study presents a simple system that can provide a large number of PLBs for mass propagation in a short time that can be converted into plants and also used for the new cultivars of Tolumnia orchids.

show abstract

Physical wounding and ethylene stimulated embryogenic stem cell proliferation and plantlet regeneration in protocorm-like bodies of Phalaenopsis orchids

Cited by 11 publications

References 40 publications

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

Phalaenopsis pollinia storage at sub-zero temperature and its pollen viability assessment

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci

Micropropagation of Tolumnia Orchids through Induction of Protocorm-like Bodies from Leaf Segments

Contact Info

Product

Resources

About