Physical state and expression of human papillomavirus in laryngeal carcinoma and surrounding normal mucosa

Abstract

Epidemiologic and biomolecular evidence suggests that human papillomavirus (HPV) infection may be associated with the development of head and neck cancers. To clarify the role of HPV in larynx carcinoma, 25 patients were studied for the presence of viral DNA, possible virus integration into the cellular genome, and viral expression both in neoplastic tissues and in neighbouring normal mucosa. Twelve of 25 patients with neoplasia (48%) showed negative results for HPV sequences, and 13 (52%) showed positive results. Among the latter group of patients, seven were HPV-16 positive, five were HPV-6, and one was HPV-45. No multiple infections were detected. The physical status of the HPV genome was analysed by three methods: polymerase chain reaction (PCR), bidimensional agarose gel electrophoresis, and in situ hybridisation. Viral integration into the host genome occurred in 43% of cases of HPV-16 and in 20% of cases of HPV-6. Viral RNA expression was detected by reverse transcription-PCR only in HPV-16-positive tumours. The pattern of expression was consistent with an active role of HPV in cellular transformation. In conclusion, the present work suggests that HPV infection may be involved in some cases of laryngeal carcinoma. However, the transformation mechanisms might be different from those currently accepted for anogenital cancers.

Human Papillomavirus in Non-Oropharyngeal Head and Neck Cancers: A Systematic Literature Review

“…Compared to oral carcinomas, a greater diversity of other HPV types has been detected: HPV18, HPV26, HPV31, HPV33, HPV39, HPV36, HPV45, HPV51, HPV52, HPV58, HPV59, HPV66, and HPV69 [8,9,14,20,26,29,39,65,71,90,94]. Low-risk HPV are uncommonly detected, and might represent incidental ''bystander'' rather than possibly ''driver'' infection [29,39,65,75,90,94].…”

“…Low-risk HPV are uncommonly detected, and might represent incidental ''bystander'' rather than possibly ''driver'' infection [29,39,65,75,90,94]. Rarely, integrated low-risk HPV has been found; viral integration does suggest viral-mediated carcinogenesis [90,94]. Head and Neck Pathol (2012) 6:S104-S120 S109 …”

Molecular analyses and prognostic relevance of HPV in head and neck tumours

“…PCR products were electrophoresed through 2% agarose gel and visualised by ethidium bromide staining under ultraviolet light. Typing of the HPV-positive samples was performed by automated sequencing of the amplified products (Biogen GeneLab, Italy) and by type specific amplification with selected primers for the E6 region of HPV16 (6). Sequence analyses of the positive samples were performed using the BLAST programme (http://www.…”

“…viral oncogene expression (6)(7)(8), integration into cell genome (6,(9)(10)(11), and viral load (10)(11)(12). Moreover, data on the simultaneous determination of all these viral characteristics are lacking.…”

Replication and Assembly of Human Papillomaviruses

“…In the case of infections of the oral cavity and/or oropharynx, HPV6, HPV11, HPV16, and HPV18 are common, with approximately 40-60% of head and neck cancers containing oncogenic HPV types such as HPV16 and HPV18 (zur Hausen, 1996;Herrero et al, 2003;Gillison, 2004;D'Souza et al, 2007;Anaya-Saavedra et al, 2008;Fakhry et al, 2008). While HPV16 is found in approximately 90% of HPVcontaining head and neck cancers, several other high-risk HPV types have been detected in these lesions (Hoshikawa et al, 1990;Niedobitek et al, 1990;Ostwald et al, 1994;Shindoh et al, 1995;zur Hausen, 1996;Mellin et al, 2000;Venuti et al, 2000;Mork et al, 2001).…”