Physical properties and biocompatibility of an injectable calcium-silicate-based root canal sealer: in vitro and in vivo study

Abstract

BackgroundThe aim of this study was to investigate the physical properties and biological effects of an experimentally developed injectable premixed calcium-silicate root canal sealer (Endoseal) in comparison with mineral trioxide aggregate (MTA) and a resin-based sealer (AHplus).MethodsThe pH, solubility, dimensional change, flow, and radiopacity of the materials were evaluated. Biocompatibility was evaluated on the basis of cell morphology and a viability test using MC3T3-E1 cells. For evaluate inflammatory reaction, the tested sealers were implanted into dorsal subcutaneous connective tissue of Sprague Dawley rats. After 7 days, the implants with the surrounding tissue were retrieved, and histological evaluation was performed.ResultsEndoseal showed high alkalinity similar to that of MTA. The solubility of the tested materials was similar. The dimensional change and flow of Endoseal was significantly higher than that of other materials (P < 0.05). The radiopacity of Endoseal was lower than that of AHplus (P < 0.05). The biocompatibility was similar to those of MTA. Inflammatory reaction of Endoseal was similar with that of MTA, but lower than that of AHplus (P < 0.05).ConclusionsThe present study indicates that Endoseal has favorable physical properties and biocompatibility. Therefore, we suggest that Endoseal has the potential to be used as a predictable root canal sealer.

Avaliação da citotoxicidade de dois cimentos à base de MTA: um estudo in vitro

“…Devido às excelentes propriedades físico-químicas e biológicas do MTA, novos materiais compostos pelo agregado trióxido mineral surgiram recentemente. [13][14][15][16] Contudo as diversas composições diferentes e as proporções distintas de MTA nas formulações promovem resultados de citotoxicidade variados a estes cimentos com o mesmo componente base. 14,17 O MTA Fillapex foi o material que apresentou a maior citotoxicidade em valores absolutos em todas as diluições e entre todos os cimentos endodônticos testados.…”

“…21 Diversos métodos vêm sendo utilizados a fim de avaliar os efeitos citotóxicos dos cimentos endodônticos. 5,9,[11][12][13][14][15][16][17] No presente estudo, a viabilidade celular foi determinada através do ensaio de MTT, que é baseado na habilidade das enzimas desidrogenases mitocondriais, presentes nas células vivas, em converter o sal amarelo tetrazólio para cristais de formazan lilás. As vantagens deste método são sua simplicidade, velocidade, precisão e elevada reprodutibilidade.…”

Endodontic Management of Pathologic Root Resorption using EndoSeal Mineral Trioxide Aggregate

“…Also, property of flow allows a sealer to penetrate into the irregularities and accessory canals of the root canal system. In the aforementioned study by Lim et al, 2 EndoSeal containing small particles of calcium silicate showed significantly higher flow values compared with the resin-based sealers. However, if the flow is excessive, the risk of sealer extrusion beyond apical foramen is increased, which could damage periodontal tissues or important anatomical structures, such as inferior alveolar nerve or maxillary sinus.…”

“…1 Endodontic sealers are used for the obturation of root canal systems in order to achieve a fluid-tight seal between the dentinal wall and core-filling material throughout the entire canal. 2 Commercially available various sealers are categorized according to chemical components: Zinc-oxide eugenol, calcium hydroxide-containing, resin-based, glassionomer-based, silicone-based, and newer generation bioceramic-based sealers. 3 As root canal sealers are placed in close contact with the surrounding soft and hard tissues for a long period CASE REPORT 10 EndoSeal (Maruchi, Korea) is a premixed and injectable endodontic sealer that uses moisture in the air to initiate the setting reaction.…”

Comparative evaluation of Fracture Resistance of Endodontically Treated Teeth Obturated with Pozzolan-based MTA Sealer and Epoxy Resin-based Sealer: An in vitro Study

“…16 The greater fracture resistance values obtained for Endoseal MTA in the present study can be attributed to its higher flow rates and biomineralization of dentinal tubules. 17 Lim et al reported that Endoseal MTA exhibited significantly higher flow compared to AH Plus sealer. Endoseal MTA is a premixed material supplied in syringes and the freshly extruded mix exhibits thixotropic behavior.…”