Physical modification approaches to enhance cell supporting potential of poly (vinyl alcohol)‐based hydrogels

Abstract

PVA‐based hydrogels with tissue engineered scaffolds application commonly need further modification to improve cell‐matrix interactions with respect to subsequent cell proliferation and differentiation. In this study, PVA‐modified hydrogels were formed by subjecting the solutions of PVA/Poly (R‐3‐hydroxybutyrate) (PHB), PVA/Extracellular Matrix (ECM), and PVA/PHB/ECM to freeze–thaw cycles and additive materials effect on cell supporting potential of the hydrogels was investigated. As, limited cell attachment and spreading were observed on PVA blended hydrogels, air plasma surface modification has been performed to promote cell attachment. Attenuated total reflection Fourier transform infrared (ATR‐FTIR) spectroscopy revealed the presence of some reactive bonds such as carbonyl on pure and amide on ECM‐coated PVA after plasma exposure. Atomic force microscopy (AFM) also proved increased roughness of hydrogel surface due to the plasma treatment. Plasma modification positive effect on cytoskeleton arrangement of cultured equine adipose derived stem cells (eASCs) was then confirmed by DAPI/phalloidin staining and scanning electron microscopy (SEM) imaging. In summary, among different physical modification approaches, coating with ECM fallowed by air plasma treatment had the most significant effect on cell‐hydrogel interactions. Thus, this combined modification method can be utilized to improve initial attachment and subsequent phenotype of cultured cells on PVA hydrogels for tissue engineering applications.

Biomimetic surface modification of Three-dimensional printed Polylactic acid scaffolds with custom mechanical properties for bone reconstruction

“…21 In contrast, surface modification methods, which do not alter the bulk structure, overcome these restrictions and considerably improve the primary attachment of cells on scaffolds. 22,23 To provide the biochemical signals necessary for the repair of bone defects, we suggested coating 3D-printed scaffolds with a biomimetic hydrogel made of collagen and biphasic calcium phosphate (BCP) ceramic, which is made from bovine bone (BCP-N) and chemical precursors (BCP-S). We found that the major phases for BCP-N and BCP-S were HA and β-TCP, respectively (Figure 1).…”

A Model of Mechanotransduction in Polyvinyl Alcohol‐Based Composite Hydrogels for Regulating Musculoskeletal Differentiation

“…The PVA solution was then cooled down to 60 °C under magnetic stirring and then cast into the 10 cm petri dishes up to a thickness of 3 mm and exposed to three cycles of freezing and thawing at −80 and 25 °C, for 6 h. PVA hydrogel was prepared according to a former published study. [ 23 ]…”

Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains

“…Alongside simultaneous measurements with MFM, the characterization of surface roughness through AFM is an important factor that dictates the affinity between cells and the surface of biomaterials. [24][25][26] In this work, we investigated the distribution of magnetic domains in crosslinked poly(vinyl alcohol) (PVA) and sodium alginate (SA) containing MNPs. PVA/SA/MNPs hydrogels were dried in the absence and in the presence of external magnetic field, in order to obtain MNPs distributed randomly (PVA/SA-rMNP) and magnetically oriented MNPs (PVA/SA-gMNP) in the polymer matrix.…”