Physical microenvironment and its effects

Fujiwara, Kazuhiro; Kozai, Toyoki

doi:10.1007/978-94-015-8461-6_15

Cited by 76 publications

(5 citation statements)

References 136 publications

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“…Fujiwara and Kozai [ 120 ], reviewing the physical microenvironment and its effects, pointed out that the temperature of the air within the culture vessels can be considered almost the same as that outside the vessels during almost all the dark period and that, during the photoperiod with cool white fluorescent lamps, the air temperature differences between the inside and outside were less than 0.5 °C with an irradiance of 60 µmol m −2 s −1 . With this data as reference, no special attention needs to be paid to the air temperature in culture vessels in conventional plant tissue culture.…”

Section: Characterization Of Led Luminaires For Use In Plant Tissue Culturementioning

confidence: 99%

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

Barceló-Muñóz

Barceló

Calderón

2021

Plants

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In the last decades, lighting installations in plant tissue culture have generally been renewed or designed based on LED technology. Thanks to this, many different light quality advances are available but, with their massive implementation, the same issue is occurring as in the 1960s with the appearance of the Grolux (Sylvania) fluorescent tubes: there is a lack of a methodological standardization of lighting. This review analyzes the main parameters and variables that must be taken into account in the design of LED-based systems, and how these need to be described and quantified in order to homogenize and standardize the experimental conditions to obtain reproducible and comparable results and conclusions. We have designed an experimental system in which the values of the physical environment and microenvironment conditions and the behavior of plant tissue cultures maintained in cabins illuminated with two lighting designs can be compared. Grolux tubes are compared with a combination of monochromatic LED lamps calibrated to provide a spectral emission, and light irradiance values similar to those generated by the previous discharge lamps, achieving in both cases wide uniformity of radiation conditions on the shelves of the culture cabins. This study can help to understand whether it is possible to use LEDs as one standard lighting source in plant tissue culture without affecting the development of the cultures maintained with the previously regulated protocols in the different laboratories. Finally, the results presented from this caparison indicate how temperature is one of the main factors that is affected by the chosen light source.

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Section: Characterization Of Led Luminaires For Use In Plant Tissue Culturementioning

confidence: 99%

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

Barceló-Muñóz

Barceló

Calderón

2021

Plants

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“…MilliSeal TM was done as previously described by Fujiwara and Kozai (1995). The headspace of each flask with different sealing type was filled with air saturated with carbon dioxide (CO2) in a concentration of 25 mmol mol -1 .…”

Section: Gas Exchange Evaluationmentioning

confidence: 99%

“…The inner concentrations of CO2 were measured with a Headspace Gas Analyzer 6600 (IIlinois Instruments, Johnsburg, IL, USA). The amount of gas exchange per hour (N') in each sealing condition was estimated by the following equation: (Fujiwara and Kozai, 1995), where T denotes the time (in hours) between two readings 1 and 2; C1 and C2 denotes to the inner CO2 concentrations in the flasks at times 1 and 2; Cout is the CO2 concentration in the environment external to the flask.…”

Section: Gas Exchange Evaluationmentioning

confidence: 99%

In vitro organogenesis in tomato cultivars is enhanced by gas exchange and application of ultrasound

Fernandes

Freitas

Batista

et al. 2020

BJD

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“…Lingkungan kultur memiliki kelembaban dan kandungan karbohidrat tinggi dengan intensitas cahaya yang rendah (Smith et al, 1986Cit Grace, 1990Kitaya et al, 1996) serta akumulasi senyawa toksik dan absennya mikroorganisme (Kitaya et al, 1996) dibandingkan dengan lingkungan rumah kaca. Kelembaban dalam botol kultur berisi planlet dapat mencapai  95% (Fujiwara and Kozai, 1995). Kelembaban yang tinggi menyebabkan terganggunya perkembangan, fisiologi, dan struktur morfologi tanaman kultur yang pada umumnya menyebabkan tingginya angka kematian tanaman saat diaklimatisasi (Kozai et al, 1993).…”

Section: Hasil Dan Pembahasan Ciri Morfologi Daununclassified

Struktur Anatomi Daun Artemisia cina Berg. Ex Poljakov Hasil Kultur Jaringan

Ermayanti¹,

Juliarni²,

Andry³

2019

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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This study was conducted to investigate the anatomical structure of leaves of Artemisia cina Berg. ex Poljakov due to variances in leaf morphology during grown in vitro. Shaking of culture, position of stem nodes used as explants and concentration of BAP (Benzyl Amino Purin) added to the culture medium were the factors investigated in this study. There was variation in leaves morphology of culture, however this variation did not occur after aclimatization and plant grown in the greenhouse. BAP decreased the level of chlorophyll content of leaves. In general, anatomical characters of leaves were not influenced by shaking, nodes position of stem and level of BAP.

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Physical microenvironment and its effects

Cited by 76 publications

References 136 publications

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

Effect of LED Lighting on Physical Environment and Microenvironment on In Vitro Plant Growth and Morphogenesis: The Need to Standardize Lighting Conditions and Their Description

In vitro organogenesis in tomato cultivars is enhanced by gas exchange and application of ultrasound

Struktur Anatomi Daun Artemisia cina Berg. Ex Poljakov Hasil Kultur Jaringan

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