This study was conducted to investigate the anatomical structure of leaves of Artemisia cina Berg. ex Poljakov due to variances in leaf morphology during grown in vitro. Shaking of culture, position of stem nodes used as explants and concentration of BAP (Benzyl Amino Purin) added to the culture medium were the factors investigated in this study. There was variation in leaves morphology of culture, however this variation did not occur after aclimatization and plant grown in the greenhouse. BAP decreased the level of chlorophyll content of leaves. In general, anatomical characters of leaves were not influenced by shaking, nodes position of stem and level of BAP.
Research on the effect of organic mulch and earthworms under oil palm plantation on the total population bacteria is still scarce. The study aims to analyze the effect of organich mulch, earthworm desity, and the interaction between organich mulch and earthworm density on the total population bacteria. The research was carried out in May – August 2019. This research was a 3 x 4 factorial experiment which was placed according to a Split Plot Design with the basic design of Randomized Block Design, each treatment was repeated 3 times. Organic mulch as the main plot are 3 type, namely: M1 (M. bracteata), M2 (oil palm empty fruit brunches/ OPEFB) and M3 (oil palm midrib). The density of earthworms as subplots consists of 4 levels, namely: C0 (0 worms/m2), C1 (35 worms/m2), C2 (50 worms/m2) and C3 (65 worms/m2). The data were analyzed using analysis of variance. If the treatment is significantly different, the treatment was tested by Duncan’s at 5% significance level. The results showed that oil palm midrib organic mulch can increase the total population of fungi and bacteria compared to M. bracteata and OPFEB. The earthworms density 50 and 65 worms/m2 can increase the total bacterial population compared to other earthworm densities. The combination of oil palm midrib with earthworms density 50 and 65 worms/m2 can increase the total population of bacteria
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