Physical Mapping and Molecular Cloning of Caulimovirus DNA

Donson, Jonathan; Hull, Roger

doi:10.1099/0022-1317-64-10-2281

Cited by 17 publications

(9 citation statements)

References 27 publications

(32 reference statements)

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“…The overall 50% nucleotide homology between CERV and CaMV is not sufficient for detection by hybridization (Donson and Hull, 1984). This is probably due to there being only short stretches of homology, the DNAs being relatively AT rich and the stringency of the hybridization conditions.…”

Section: Discussionmentioning

confidence: 98%

“…The DNA has been mapped using restriction endonucleases and shown to be 8 kbp and to have the three single-stranded discontinuities (gaps), one (GI) in one strand and two (G2 and G3) in the other, characteristic of CaMV (Hull and Donson, 1982). However, hybridization studies showed that CERV DNA apparently has only a little sequence homology with that of CaMV (Donson and Hull, 1984).…”

Section: Introductionmentioning

confidence: 99%

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The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses

1986

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Carnation etched ring virus (CERV) DNA comprises 7932 bp. CERV primer binding sites and overall genome organization are similar to those of the related cauliflower mosaic virus (CaMV). The six open reading frames of CERV showed amino acid homology (50‐80%) with CaMV ORFs I‐VI; no homologues of CaMV ORFs VII or VIII were found. CERV ORFs 1‐5 interface each other with the sequence ATGA. The comparison of CERV ORF5 with CaMV ORFV highlighted regions which show homologies to retrovirus gag/pol protease, RNase H and DNA polymerase domains; the possibility that the DNA polymerase domain comprises two subdomains, operating off different templates, is discussed. Both CERV and CaMV ORFs I have sequence homology to tobacco mosaic virus P30 and plastocyanin.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses

1986

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“…PCISV-CVB DNA is very similar in size to soybean chlorotic mottle vims (SoCMV) (8 2 kbp) (Hibi et al, 1989) and PCISV (8-2 kbp) DNAs (Reddy et at,, 1993;Richins, unpublished data). As observed in the case of PCISV, the restriction map of PCISV-CVB DNA revealed no similarity to other caulimoviruses (Meagher et at,, 1977;Volovitch et at., 1979;Hull, 1980;Hull & Donson, 1982;Donson & Hull, 1983;Richins & Shepherd, 1983;Hibi et at., 1986). The differences that exist between the physical maps of PCISV (Reddy et al 1993) and that constructed for PCISV-CVB and the differences in host range and reaction lend support to our conclusion that PCISV-CVB is a distinct strain of PCISV, PCISV-CVB DNA was cloned into pUC 118, A full-length clone pPClSV-CVB was obtained that produced 10-12 local chlorotic lesions per cowpea plant when excised from the cloning vector.…”

Section: Resultsmentioning

confidence: 99%

Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

et al. 1995

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The double‐stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak virus (PCISV‐CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCISV‐CVB DNA to be circular and composed of approximately 8·2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCISV, Nevertheless, several differences between physical maps of the two viruses suggest that PCISV‐CVB should be considered as a distinct strain of PCISV. Bam HI‐cleaved PCISV‐CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata.

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“…1 a, and in Turner & Covey, 1993). We suspected this heterogeneity was due to the twisted conformation exhibited by CaMV virion DNA which produces multiple bands during one-dimensional neutral electrophoresis (Hull & Howell, 1978;Donson & Hull, 1983). The mobility of CaMV virion DNA was tested by 2D electrophoresis to determine how the twisted forms behave (Fig.…”

Section: Twisted Forms Of Camv Dnamentioning

confidence: 99%

Changes in populations of cauliflower mosaic virus DNA and RNA forms during turnip callus proliferation

Covey

Turner

1993

Journal of General Virology

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Cauliflower mosaic virus (CaMV) nucleic acids accumulate in the cell in different structural conformations related to their roles in gene expression, replication and virion assembly. We have characterized changes in the population CaMV DNA and RNA replication products which occur following culture of infected turnip leaves under conditions where callus proliferates. After only 5 days in culture, a significant increase in the level of genome-length and subgenomic supercoiled (SC) DNA forms was observed by two-dimensional (2D) gel electrophoresis. Open circular (OC) molecules, corresponding to these SC DNAs, with mobilities consistent with the presence of a single break in each strand, were also detected after 5 days culture. By 10 days culture, the proportion of OC molecules with only one break per double-stranded molecule had increased. After 34 days culture, SC DNA with a range of sizes predominated in the unencapsidated DNA fraction. The change in pattern of OC and SC DNA forms during callus proliferation suggests a possible precursor/product relationship involving generation of deleted molecules from gapcontaining virion DNA-like molecules followed by sequential repair of the gaps to produce SC DNA. Moreover, heterogeneity in the mobility of OC DNAs in the neutral dimension of 2D electrophoresis, a feature exhibited by twisted CaMV virion DNA, changed during the time-course suggesting that untwisting occurs during gap repair. Although the relative abundance of SC DNA increased during callus proliferation, CaMV polyadenylated 35S and 19S transcripts declined together with immediate reverse transcription products. We suggest that cellular changes during callus growth lead to a decline in authentic CaMV transcripts in the cytoplasm resulting in cessation of synthesis of viral products and progeny DNA genomes. In consequence, pre-existing virion DNAs return to the nucleus, possibly as a result of a relaxation in a cytoplasmic control mechanism, where they are assembled into various forms of SC DNA. The presence of CaMV SC DNAs in replicating cells might also enhance illegitimate integration into host chromosomes, as hybridization ofCaMV DNA to high M r DNA was observed.

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Physical Mapping and Molecular Cloning of Caulimovirus DNA

Cited by 17 publications

References 27 publications

The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses

The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses

Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

Changes in populations of cauliflower mosaic virus DNA and RNA forms during turnip callus proliferation

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