Genome characterization of a new strain of peanut chlorotic streak virus causing chlorotic vein banding disease of groundnut (Arachis hypogaea L.) in India

Abstract

The double‐stranded DNA of the chlorotic vein banding isolate of peanut chlorotic streak virus (PCISV‐CVB), isolated from purified virus, resolved into circular and linear molecules similar to those of other caulimoviruses. A physical map of viral DNA was constructed, which showed the PCISV‐CVB DNA to be circular and composed of approximately 8·2 kbp. A number of restriction sites were found to be shared with a similar caulimovirus, PCISV, Nevertheless, several differences between physical maps of the two viruses suggest that PCISV‐CVB should be considered as a distinct strain of PCISV. Bam HI‐cleaved PCISV‐CVB DNA was cloned into pUC 118 and was infectious when cleaved from the cloning vector and inoculated onto Vigna unguiculata.

Pothur Sreenivasulu (1950–2020): a humble virologist who made eminent contributions to virology discipline in India

“…To accomplish this task, he has established successful collaborations with many institutions within India and abroad, including with ICRISAT, Indian Institute of Science (IISc) in Bangalore, a regional research station in Acharya NG Ranga Agricultural University in Kovvur, Indian Institute of Spices Research in Calicut, Maharashtra Hybrid Seeds Company in Jalna, University of Delhi-South Campus, and virology programs in the USA, UK and Belgium. Some of his notable achievements are on of peanut chlorotic leaf streak caulimovirus-chlorotic vein banding isolate (PCLSV-CVB) [4], cowpea mild mottle carlavirus (CPMMV), sugarcane mosaic potyvirus (SCMV), sugarcane streak mosaic poacevirus (SCSMV), rice tungro virus complex, maize stripe tenuivirus (MSpV), konjac mosaic potyvirus (KoMV) isolates, spathiphyllum chlorotic vein banding potyvirus (SCVbV), banana bract mosaic potyvirus (BBrMV) isolates, peanut stripe potyvirus (PStV), peanut green mosaic potyvirus isolates (PGMV), cucumber mosaic cucumovirus isolates, pegionpea sterility mosaic emaravirus, and Sreenivasulu was a modest, kind-hearted, and fair person. His impeccable integrity and commitment to achieve perfection are commendable.…”