Physical map of chromosomal and plasmid DNA comprising the genome of<i>Leptospira interrogans</i>

Zuerner, Richard L.

doi:10.1093/nar/19.18.4857

Cited by 50 publications

(59 citation statements)

References 18 publications

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“…However, the physical map constructed in this study demonstrates the circular nature of the L. interrogans chromosome. In addition, during the course of these studies, the physical map of the chromosome of L. interrogans serovar pomona strain Kennewicki was reported and shown to be circular (41 In agreement with the results of others (36,41), our analysis of the whole genome of L. interrogans showed the presence of an extrachromosomal element. A 350-kb band is seen in undigested preparations and in AscI digests of L.…”

Section: Discussionsupporting

confidence: 90%

“…Taylor et al suggested that the L. interrogans chromosome is linear, fragmented, and approximately 3.1 Mbp (36). However, we found it to be circular and 4.6 Mbp, in agreement with our earlier results (2) and the report of Zuerner (41). Such a map will provide a point of reference from which the linkage of specific genes can be established.…”

supporting

confidence: 93%

“…It is hidden in NotI and S-fl digests because of its size, identical to that of NtC and SrE, respectively. This giant plasmid may be homologous to the extrachromosomal element reported in other Leptospira serovars (36,41). However, further study is needed to ascertain its circular or linear structure, as conflicting results have been reported.…”

Section: Discussionmentioning

confidence: 79%

“…Bands NtB, NtC, and NtJ disappeared and were replaced by NtB' and NtC' at 1,100 and 700 kb (Fig. 3, lane 2 (36,41), corresponded to the 0.35-Mbp band seen both with undigested DNA and after AscI digestion (AsC). This extrachromosomal element should also be visible in the NotI digest pattern.…”

Section: Resultsmentioning

confidence: 93%

“…The 350-kb plasmid found in L. interrogans serovars canicola, pomona, icterohaemorrhagiae, and grippotyphosa was reported to be linear (36). The physical map of plasmid pLIN1 (350 kb) found in L. interrogans serovar pomona is circular (41). pLIN1 has a copy' number of 1 or 2 per chromosome (41).…”

Section: Discussionmentioning

confidence: 99%

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Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

et al. 1992

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Leptospira interrogans is a pathogenic bacterium with a low G+C content (34 to 39%). The restriction enzymes NotI, AscI, and SrfI cut the chromosome of L. interrogans serovar icterohaemorrhagiae into 13, 3, and 5 fragments separable by one- and two-dimensional pulsed-field gel electrophoresis (PFGE). The genome is composed of a circular 4.6-Mbp chromosome and a 0.35-Mbp extrachromosomal element. A physical map of the chromosome was constructed for NotI, AscI, and SrfI by using single and double digests, or partial NotI digests obtained at random or by cross-protection of NotI sites by FnuDII methylase, and linking clones. rRNA genes were found to be widely scattered on the chromosome.

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Section: Discussionsupporting

confidence: 90%

supporting

confidence: 93%

Section: Discussionmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

et al. 1992

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Leptospira

Adler¹,

Faine²

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Leptospira

Zuerner¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Lep.to.spi'ra. Gr. adj. leptos thin, narrow, fine; L. fem. n. spira a coil, helix; N.L. fem. n. Leptospira a thin helix or coil, referring to the morphology of the bacterium. Spirochaetes / Spirochaetia / Spirochaetales / Leptospiraceae / Leptospira Leptospira are long, thin, flexible rods, 0 . 1 μ m in diameter and 6–12 μ m in length , with a regular right‐handed helical coiling pattern (Carleton et al., 1979). These bacteria are unicellular but may be observed as dividing pairs or short chains of three or more cells in actively growing cultures. Resting stages are not known, but long term survival in water, with the appearance of aggregates has been described (Trueba et al., 2004). Spherically shaped cells form under unfavorable growth conditions. Bacteria stain as Gram‐negative. Due to the small diameter of these bacteria, unstained cells are not visible by bright‐field microscopy . Dark‐field or phase‐contrast microscopy is required for visualization of unstained cells. These are highly motile aerobic or microaerophilic bacteria. Optimum growth temperature is 28–30°C, with a generation time of 6–16 h, although many primary pathogenic isolates may grow slower . Chemoorganotrophic bacteria that consume long‐chain fatty acids and alcohols as primary carbon and energy sources, and carry out respiration with oxygen as the terminal electron acceptor. Optimal growth occurs in semi‐solid (0.1–0.2%) agar media. Growth on 1–2% solid agar results in the formation of clear to turbid surface or subsurface colonies. Colony formation is enhanced by the addition of pyruvate. Oxidase, catalase, and/or peroxidase‐positive. Some strains are β‐hemolytic. Some strains are pathogenic for humans and animals, while other strains are saprophytic and found in freshwater and marine environments. The genus Leptospira forms a deep unique branch of spirochetes, separate from other genera based on comparison of 16S rRNA gene sequences . Species are differentiated by DNA–DNA relative reassociation analysis and by unique sequence polymorphisms in 16S rRNA. DNA G + C content ( mol %): 35–43. Type species : Leptospira interrogans (Stimson 1907) Wenyon 1926, 1281 emend. Faine and Stallman 1982, 462 ( Spirochaeta interrogans Stimson 1907, 541).

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Physical map of chromosomal and plasmid DNA comprising the genome ofLeptospira interrogans

Cited by 50 publications

References 18 publications

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae

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