Physical, chemical and biological properties of a specific toxic lipid-protein complex formed in thermally altered mouse skin

Schoenenberger, G. A.; Bauer, Ulf; Cueni, L. B.; Eppenberger, Urs; Städtler, K; Allgöwer, M.

doi:10.1016/0005-2795(72)90170-5

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…The source for human burn toxin was skin split into layers 1 mm thick [10]. Both toxins were isolated and characterized by, a technique previously elaborated in our laboratories [13,14] and both toxins were tested for intrinsic toxicity in a model shown to give an all-or-none response, i.e., 100% mortality [16].…”

Section: Methodsmentioning

confidence: 99%

“…Group 2 received an identical amount of the nontoxic precursor derived from not burned or normal skin by the same isolation procedure as described for the toxin isolation [14] under otherwise identical conditions. Group 3 was injected i.p.…”

Section: In the Second Set O/experiments Three Groups O] 10 Animmentioning

confidence: 99%

“…During the period (1960--1970) covered by this report Ill], the overall mortality seemed rather to be proportional to the percentage of body surface burned as one would expect when assuming a causal relationship between mortality rate and a specific toxic compound produced in the skin by thermal energy. These facts raised the question of whether immunotherapy had lowered the septicemia and whether mouse burn toxin isolated, purified, and characterized in our laboratories [13,14] would influence the host defense against psendomonas under experimental conditions. The occurrence of a similar burn toxin in human skin also has been demonstrated [10].…”

Section: Introductionmentioning

confidence: 98%

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Specific impairment of host defense for pseudomonas infection in mice by a burn toxin derived from thermally altered human skin

Müller¹,

Allgöwer²,

Städtler³

et al. 1974

Res. Exp. Med.

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Summary. The mortality rate for an infection with standardized increasing amounts of a characterized strain of Pseudomonas aeruginosa has been established.In these experiments, 1 × 10 T organisms, causing a reproducible mortality rate of 0--20~o in normal control animals, were used. The mortality of mice due to this standardized pseudomonas infection was significantly increased by preinjection of an otherwise sublethal dose of a specific burn toxin 72 hrs before the organisms were applied in a standard wound pocket. The same effect was demonstrated in mice using a specific burn toxin isolated from human skin. This increased mortality rate was counteracted with a specific hcterologous antitoxic IgG and normalized to the mortality rate of the corresponding controls. A possible pathogenic effect of the isolated toxin, namely generalized damage to the cell wall membranes may enhance the establishment of septicemia and may suggest a way for improving the survival rate of severely burned patients.Key words: Burns --Infection --Burn toxin --Therapy --Antitoxic serum. Zusammen]assung. Die prozentuale Sterbliehkeit von M~usen wurde nach einerInfektion mit steigenden Keimzahlen eines definierten Pseudomonas aeruginosaStammes bestimmt. 1 × l0 T Keime bewirken den Ted yon 0~20°/o der Tiere. Diese Keimzahl wurde als Standard fiir die weiteren Versuche verwendct. Die Sterblichkeitsrate der MSmse nach dieser standardisierten Pseudomonas-Infektion war signifikant erhSht, wenn die Tiere 72 Std vor der Applikation der Keime in eine Wundtasche am Riicken eine einmalige, kleine, an und ftir sich harmlose Gabe eines spezifischen, aus M~Lusehaut isolierten Verbrennungstoxins intraperitoneal erhielten. Die gleiche Wirkung wurde mit einem aus verbrannter menschlicher Haut mittels desselben Isolierungsverfahrens gewonnenen Toxin erreicht. Die Steigerung der Sterbequote wurde durch ein spezifisches, heterologes antitoxisches IgG verhindert,

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Section: Methodsmentioning

confidence: 99%

Section: In the Second Set O/experiments Three Groups O] 10 Animmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Specific impairment of host defense for pseudomonas infection in mice by a burn toxin derived from thermally altered human skin

Müller¹,

Allgöwer²,

Städtler³

et al. 1974

Res. Exp. Med.

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“…The toxin, however, differed from the native product in its larger molecular size and higher density; thus, it was shown that con trolled thermal energy causes a polymerization of the naturally occurring monomer into a polymer, i.e. the burn toxin [15], The biological and path ogenic properties of the isolated compound have now been investigated in vivo.…”

Section: Introductionmentioning

confidence: 99%

Formation of a Specific ‘Burn Toxin’ in Mouse Skin by Thermal Injuries

Städtler¹,

Allgöwer²,

Cueni³

et al. 1972

Eur Surg Res

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A new technique to apply standard high temperature burn injuries and scalds under controlled conditions in living mice has been established. The difference between scalds and ‘dry’ heat injuries in terms of the mortality rate and the histological changes within the skin has been demonstrated. The relationship between the surface area injured and the animal’s body weight and total surface as a critical parameter with respect to survival or mortality has been established. A mechanism for toxin formation in mammalian skin after high temperature energy input has been offered. The physical and chemical characteristics of a specific burn toxin have been described. The toxic effect of the isolated product and the lethal burn injury in vivo upon kidney tissue, serum creatinine and urea have been shown. A direct correlation between the toxic compound and the mortality has been shown by protecting the animals from the toxin (a) by delayed toxin absorption; (b) by active immunization of the animals against the toxin, and (c) by serum (IgG) therapy using heterologous and homologous anti-toxic serum.

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