Physical characteristics and NaCl content of urine white male rat (Rattus norvegicus L.) after orally intakes of cogon grass rhizome (Imperata cylindrica L.) extract

Abstract

The aims of this research were to find out the effects of orally intakes of cogon grass rhizome (Imperata cylindrica L.) extract on physical characteristics and NaCl content of urine, and the dosage of the extract that able to significantly change the physical characteristics and the content of NaCl on the urine white males rat (Rattus norvegicus L.). Complete Randomized Design with six groups and five replications to each group was used in this study. The treatment applied for those groups were: aquadest 2 ml/200 g BW, HCT 0,32 mg/200 g BW, CMC 2% 2 ml/200 g BW, the cogon grass rhizome extract 5 mg/200 g BW, the cogon grass rhizome extract 10 mg/200 g BW and the cogon grass rhizome extract 20 mg/200 g BW for groups I to VI respectively. The parameters used for the physical characteristics of the urine were volume, color, clearness, pH, density and urine sediments. Analysis of NaCl content was done by Fantus method. Data collected were then analyzed using ANOVA and continued with DMRT test at the level of 5% significance. The result showed that orally intakes of cogon grass rhizome extract at all level dosages (5 mg/200 g BW, 10 mg/200 g BW and 20 mg/200 g BW) were significantly increased the volume of urine and reduced of NaCl content, but no effect on pH and density of the urine. Orally intakes of cogon grass rhizome extract have also resulted in changing of color, clearness and amount of urine sediment components.

Isolation and identification of a flavonoid compound and <em>in&nbsp;vivo</em> lipid‑lowering properties of <em>Imperata&nbsp;cylindrica</em>

“…The present study utilized a randomized post-test only control group experimental design. Group 1 (K0): A negative control group that received a control diet (CD); group 2 (K1): A positive control group that received a high cholesterol diet (HD); group 3 (K2): A treatment group that was administered a HD and the ethanol extract of I. cylindrica at a dose of 15 mg/200 g body weight (BW); and group 4 (K3): A treatment group that was administered a HD and the ethyl acetate I. cylindrica fraction at a dose of 15 mg/200 g BW [based on Suratman et al (21) with modifications].…”

“…Ethanol was used due to its very low toxicity. The present study employed a single dose of 15 mg/200 g BW, according to the method described by Suratman et al (21) with slight modifications. Ethanol extracts were composed of various compounds, while the ethyl acetate fraction was a fraction comprised of semipolar compounds.…”