Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

Marcos, J.; Perrotti, Vittoria; Iaculli, Flavia; Aragones, Águedo; Benfatti, César Augusto Magalhães; Magrin, Gabriel Leonardo; Piattelli, Adriano; Bianchini, Marco Aurélio

doi:10.3390/app9204296

Cited by 7 publications

(3 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was expected since homogenous dispersion of C-nHA particles in PCL scaffolds plays a role as reinforcement, which improves the mechanical properties of PCL matrix [80]. Collagen immobilization improves the mechanical resistance of scaffold materials [81]. Our results agree with these findings, since we found that surface modification significantly improved compressive strength of 3D-printed PCL/C-nHA60% scaffolds.…”

Section: Discussionsupporting

confidence: 89%

Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Moghaddaszadeh¹,

Seddiqi²,

Najmoddin³

et al. 2021

Biomed. Mater.

View full text Add to dashboard Cite

A challenging approach of three-dimensional (3D)-biomimetic scaffold design for bone tissue engineering is to improve scaffold bioactivity and mechanical properties. We aimed to design and fabricate 3D-polycaprolactone (PCL)-based nanocomposite scaffold containing a high concentration homogeneously distributed carbonated-nanohydroxyapatite (C-nHA)-particles in combination with immobilized-collagen to mimic real bone properties. PCL-scaffolds without/with C-nHA at 30%, 45%, and 60% (wt/wt) were 3D-printed. PCL/C-nHA60%-scaffolds were surface-modified by NaOH-treatment and collagen-immobilization. Physicomechanical and biological properties were investigated experimentally and by finite-element (FE) modeling. Scaffold surface-roughness enhanced by increasing C-nHA (1.7 – 6.1-fold), but decreased by surface-modification (0.6-fold). The contact angle decreased by increasing C-nHA (0.9 – 0.7-fold), and by surface-modification (0.5-fold). The zeta potential decreased by increasing C-nHA (3.2-9.9-fold). Average elastic modulus, compressive strength, and reaction force enhanced by increasing C-nHA and by surface-modification. FE modeling revealed that von Mises stress distribution became less homogeneous by increasing C-nHA, and by surface-modification. Maximal von Mises stress for 2% compression strain in all scaffolds did not exceed yield stress for bulk-material. 3D-printed PCL/C-nHA60% with surface-modification enhanced pre-osteoblast spreading, proliferation, collagen deposition, alkaline phosphatase activity, and mineralization. In conclusion, a novel biomimetic 3D-printed PCL-scaffold containing a high concentration C-nHA with surface-modification was successfully fabricated. It exhibited superior physicomechanical and biological properties, making it a promising biomaterial for bone tissue engineering.

show abstract

Section: Discussionsupporting

confidence: 89%

Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Moghaddaszadeh¹,

Seddiqi²,

Najmoddin³

et al. 2021

Biomed. Mater.

View full text Add to dashboard Cite

show abstract

“…Researchers reported that lower porosity results in cell aggregation that develops osteogenesis; similarly, higher porosity develops bone ingrowth and diminished mechanical properties [53]. Researchers proved that more voids present in the composite structure develops non-uniform distribution of loads on the composite structure, which leads to reduced mechanical properties [54]. The porosity percentage of GF/SF/CTS composite scaffolds is shown in Table 5.…”

Section: Porosity Of the Composite Scaffoldsmentioning

confidence: 99%

Investigations on the Mechanical Properties of Glass Fiber/Sisal Fiber/Chitosan Reinforced Hybrid Polymer Sandwich Composite Scaffolds for Bone Fracture Fixation Applications

et al. 2020

View full text Add to dashboard Cite

This study aims to explore the mechanical properties of hybrid glass fiber (GF)/sisal fiber (SF)/chitosan (CTS) composite material for orthopedic long bone plate applications. The GF/SF/CTS hybrid composite possesses a unique sandwich structure and comprises GF/CTS/epoxy as the external layers and SF/CTS/epoxy as the inner layers. The composite plate resembles the human bone structure (spongy internal cancellous matrix and rigid external cortical). The mechanical properties of the prepared hybrid sandwich composites samples were evaluated using tensile, flexural, micro hardness, and compression tests. The scanning electron microscopic (SEM) images were studied to analyze the failure mechanism of these composite samples. Besides, contact angle (CA) and water absorption tests were conducted using the sessile drop method to examine the wettability properties of the SF/CTS/epoxy and GF/SF/CTS/epoxy composites. Additionally, the porosity of the GF/SF/CTS composite scaffold samples were determined by using the ethanol infiltration method. The mechanical test results show that the GF/SF/CTS hybrid composites exhibit the bending strength of 343 MPa, ultimate tensile strength of 146 MPa, and compressive strength of 380 MPa with higher Young’s modulus in the bending tests (21.56 GPa) compared to the tensile (6646 MPa) and compressive modulus (2046 MPa). Wettability study results reveal that the GF/SF/CTS composite scaffolds were hydrophobic (CA = 92.41° ± 1.71°) with less water absorption of 3.436% compared to the SF/CTS composites (6.953%). The SF/CTS composites show a hydrophilic character (CA = 54.28° ± 3.06°). The experimental tests prove that the GF/SF/CTS hybrid composite can be used for orthopedic bone fracture plate applications in future.

show abstract

“…The sterilization process is carried out before and after the PLA preparation. (Marcos et al, 2019) Add 100 μl of the fixing solution, to each well. This is carried out at room temperature for 30 min.…”

Section: Chemicalsmentioning

confidence: 99%

Biocompatibility of Polylactic Acid as a Bone Substitute: An In Vitro Study

Meenapriya¹

2020

Biosci. Biotech. Res. Comm

View full text Add to dashboard Cite

Biocompatibility is defined as the ability of the bone material to perform appropriate host response in specific applications. Different bone substitutes can be used. These bone substitutes are derived from biological products as well as the synthetic products. The biological products are derived from the demineralized bone matrix, hydroxyapatite, growth factors and the synthetic products are derived from tri-calcium phosphate ceramics, or polymer based substitutes. This study aims at the biocompatibility of poly lactic acid which can be used as a bone substitute. The L929 Fibroblast cell lines were used for assessing the biocompatibility. The cell lines are maintained in a humidified atmosphere at 37degree C. Cell viability is assessed using MTT assay and the cell growth is assessed using the BrDu cell proliferation assay. The readings are done in ELISA readers at 570nm and 450nm respectively. The results are statistically analyzed by ANOVA. Even in the minimum concentration, 89.4% of the cells are viable and in the maximum concentration 100.7% of the cells are viable. The cell growth increased as the concentrations of the PLA increased. The in vitro biocompatibility of PLA exhibited satisfying biological security with favorable cell adhesion, spreading and proliferation.

show abstract

Physical and Mechanical Properties of Composite Scaffolds with or without Collagen Impregnation

Cited by 7 publications

References 40 publications

Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Biomimetic 3D-printed PCL scaffold containing a high concentration carbonated-nanohydroxyapatite with immobilized-collagen for bone tissue engineering: enhanced bioactivity and physicomechanical characteristics

Investigations on the Mechanical Properties of Glass Fiber/Sisal Fiber/Chitosan Reinforced Hybrid Polymer Sandwich Composite Scaffolds for Bone Fracture Fixation Applications

Biocompatibility of Polylactic Acid as a Bone Substitute: An In Vitro Study

Contact Info

Product

Resources

About