Physical and chemical study of coated Candida cylindracea with sorbitan esters

Abstract

A new effective biocatalyst was prepared by coating lipase from Candida cylindracea with sorbitan esters such as span 60 and 65. This biocatalyst was characterized by employing the Scanning Electron Microscopy, X-Ray diffraction patterns and a lipase assay. The type of surfactant used during the lipase modification process has shown significant effect in the lipase activity and the protein content of the modified lipase complex. It was found that catalytic behavior of modified lipase was greatly affected by the source of the used span. Span 65-coated Candida cylindracea lipase showed the highest conversion of 50% in enzymatic esterification of glucose and lauric acid.